High-throughput Agrobacterium-mediated barley transformation

Abstract

Background: Plant transformation is an invaluable tool for basic plant research, as well as a useful technique for the direct improvement of commercial crops. Barley (Hordeum vulgare) is the fourth most abundant cereal crop in the world. It also provides a useful model for the study of wheat, which has a larger and more complex genome. Most existing barley transformation methodologies are either complex or have low (<10%) transformation efficiencies.

Direct links between the vernalization response and other key traits of cereal crops

“…Differential expression analysis was performed using DEseq 53 (version 1.12.1) and edgeR 54 Gene copy number was assayed using the same protocol as qRT-PCR, but with primers targeting the transgene (Supplementary Table 2). The CO2 gene, described previously as a single-copy reference gene 55 , was used for normalization (Supplementary Table 2). …”

Genetic Transformation of Triticeae Cereals for Molecular Farming

“…However, transformation efficiency remains still variable and rather genotype dependent. In barley, the most readily transformed cultivar is 'Golden Promise', which allows an average of >10 independent transformation events per immature embryo (Bartlett et al, 2008;Hensel et al, 2009;Murray et al, 2004); other cultivars, while being amenable to transformation, show a lower level of efficiency (Hensel et al, 2008;Murray et al, 2004). One suggested means of overcoming this genotype dependency was to replace immature embryos with isolated ovules as the recipient tissue.…”

In vitro regeneration, Agrobacterium-mediated transformation, and genetic assay of chalcone synthase in the medicinal plant Echinacea pallida

“…3, 4a). In addition, herb, floricultural plant, wheat, triticale, orchid and barley transgenic plants have been shown to carry incomplete T-DNAs after Agrobacterium-mediated transformation (Bartlett et al 2008;Hensel et al 2012;Tsai et al 2012;Wang et al 2012Wang et al , 2015.…”