High sucrose consumption potentiates the sub-acute cadmium effect on Na+/K+-ATPase but not on δ-aminolevulinate dehydratase in mice

Folmer, Vanderlei; Santos, Francielli Weber; Savegnago, Lucielli; Brito, Verônica Bidinotto; Nogueira, Cristina W.; Rocha, João Batista Teixeira da

doi:10.1016/j.toxlet.2004.06.002

Cited by 27 publications

(15 citation statements)

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“…The use of thiobarbituric acid reactive substances (TBARS) assay has been carried out in plasma, 30,46-51 serum, 52 different tissues, 28,47,48,53 and occasionally in urine. 54 Urinary MDA is excreted mainly in form of adducts with lysine and its N-acetylated derivative, indicating an association with proteins.…”

Section: Biological Samples the Choice Of The Sample For Mda Determinmentioning

confidence: 99%

“…48 In fact, the tissue auto-oxidation in vitro can be used to assess its susceptibility to oxidative stress. 53,55 The evaluation of the lipid peroxidation in tissues is a good marker to measure the extent of the oxidative stress, but it is important to consider that these biological samples can only be used in experimental studies. Therefore, plasma sample is still the most used.…”

Section: Biological Samples the Choice Of The Sample For Mda Determinmentioning

confidence: 99%

“…20,68,69 Thus, it could be supposed that the use of colorimetric or fluorimetric techniques may give mistaken results, particularly in situations such as initial stages of human diseases, where lipid peroxidation can be smaller when compared to the non-specific background reaction between TBA and products not derived from lipid peroxidation. 53 …”

Section: 64mentioning

confidence: 99%

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Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification

Grotto¹,

Maria²,

Valentini³

et al. 2009

Quím. Nova

343

251

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Recebido em 16/2/07; aceito em 7/3/08; publicado na web em 31/10/08Free radicals induce lipid peroxidation, playing an important role in patho logical processes. The injury mediated by free radicals can be measured by conjugated dienes, malon dial dehyde, 4-hydroxynonenal, and others. However, malondialdehyde has been pointed out as the main product to evaluate lipid peroxidation. Most assays determine malondialdehyde by its reaction with thiobarbituric acid, which can be mea sured by indirect (spectrometry) and direct methodologies (chromatography). Though there is some controversy among the methodologies, the selective HPLC-based assays provide a more reliable lipid peroxidation measure. This review describes significant aspects about MDA deter mination, its importance in pathologies and biological samples treatment.

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Section: Biological Samples the Choice Of The Sample For Mda Determinmentioning

confidence: 99%

Section: Biological Samples the Choice Of The Sample For Mda Determinmentioning

confidence: 99%

See 1 more Smart Citation

Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification

Grotto¹,

Maria²,

Valentini³

et al. 2009

Quím. Nova

343

251

View full text Add to dashboard Cite

show abstract

“…These results are in agreement with the data obtained on δ-ALA-D activity in vitro. δ-ALA-D, a sulfhydryl enzyme, is sensitive to oxidant agents and to pro-oxidant situations Folmer et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Vinylic telluride derivatives as promising pharmacological compounds with low toxicity

Borges

Savegnago

Pinton

et al. 2008

J of Applied Toxicology

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The aim of the present study was to evaluate pharmacological and toxicological properties of (Z)-2-(methylthio)-1-(butyltelluro)-1-phenylethene 1a, (Z)-1-(4-methylphenylsulfonyl)-2-(phenyltelluro)-2-phenylethene 1b, (Z)-2-(butyltelluro)-1-(benzylthio)-1-heptene 1c and (Z)-2-(phenylthio)-1-(butyltelluro)-1-phenylethene 1d. In vitro, vinylic telluride derivatives 1a, 1d and 1c were more effective in reducing lipid peroxidation than compound 1b. The maximal inhibitory effect of vinylic telluride derivatives on lipid peroxidation was in the following order: 1a = 1d > 1c > 1b. Compound 1b was more potent in inhibiting delta-ALA-D activity (delta-aminolevulinate dehydratase) than compounds 1c and 1d. Based on the in vitro properties presented by compounds 1a (an antioxidant) and 1b (a pro-oxidant), toxicological parameters were assessed in vivo and ex vivo in rats. Calculated LD50 of compounds 1a and 1b, administered by oral route, were 20.5 and 1.44 micromol kg(-1), respectively. Compound 1b induced behavioral alterations in the open field test. Renal and spleenic delta-ALA-D activities were inhibited in rats treated orally with compound 1a. Compound 1b stimulated delta-ALA-D activity in liver and spleen of rats. Rats treated with compound 1b had increased hepatic, renal and spleenic lipid peroxidation. Renal and hepatic markers were not altered when compounds 1a and 1b were administered to rats at doses of around LD50, while compound 1a at high doses changed aspartate aminotransferase activity and urea levels. Based on in vitro results, this study demonstrated that compounds 1a and 1d are promising antioxidant compounds. Ex vivo data reinforce compound 1a as a promising drug for more detailed pharmacological studies.

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“…In addition, Na + , K + -ATPase could also be sensitive to oxidizing agents (The´venod & Friedmann, 1999;Folmer et al 2004;Borges et al 2005). Na + , K + -ATPase is an enzyme embedded in the cell membrane and responsible for the active transport of sodium and potassium ions.…”

Section: Introductionmentioning

confidence: 99%

DMPS and N-acetylcysteine induced renal toxicity in mice exposed to mercury

et al. 2006

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Acute effects of mercuric chloride (HgCl2) were evaluated on mice. Mice received a single dose of HgCl2 (4.6 mg/kg, subcutaneously) for three consecutive days. Thirty minutes after the last injection with HgCl2, mice received one single injection of 2,3-dimercapto-1-propanesulfonic acid (DMPS) or N-acetylcysteine (NAC) or diphenyl diselenide (PhSe)2. DMPS, NAC and (PhSe)2 were utilized as therapy against mercury exposure. At 24 h after the last HgCl2 injection, blood, liver and kidney samples were collected. delta-Aminolevulinate dehydratase (delta-ALA-D) and Na+, K- (+) ATPase activities, thiobarbituric acid-reactive substances (TBARS), non-protein thiols (NPSH) and ascorbic acid concentrations were evaluated. Plasma aspartate (AST) and alanine (ALT) aminotransferase activities, as well as urea and creatinine levels were determined. The group of mice exposed to Hg + (PhSe)2 presented 100% of lethality. Exposure with HgCl2 caused a decrease on the body weight gain and treatments did not modify this parameter. delta-ALA-D, AST and ALT activities, TBARS, ascorbic acid levels and NPSH (hepatic and erythrocytic) levels were not changed after HgCl2 exposure. HgCl2 caused an increase in renal NPSH content and therapies did not modify these levels. Mice treated with (PhSe)2, Hg + NAC and Hg + DMPS presented a reduction in plasma NPSH levels. Creatinine and urea levels were increased in mice exposed to Hg + NAC, while Hg + DMPS group presented an increase only in urea level. Na+, K- (+) ATPase activity was inhibited in mice exposed to Hg + DMPS and Hg + NAC. In conclusion, therapies with (PhSe)2, DMPS and NAC following mercury exposure must be better studied because the formation of more toxic complexes with mercury, which can mainly damage renal tissue.

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High sucrose consumption potentiates the sub-acute cadmium effect on Na+/K+-ATPase but not on δ-aminolevulinate dehydratase in mice

Cited by 27 publications

References 58 publications

Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification

Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification

Vinylic telluride derivatives as promising pharmacological compounds with low toxicity

DMPS and N-acetylcysteine induced renal toxicity in mice exposed to mercury

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