High-speed AFM for scanning the architecture of living cells

Li, Jing; Deng, Zhifeng; Chen, Daixie; Ao, Zhuo; Sun, Qingqing; Feng, Jie; Yin, Bohua; Han, Li; Han, Dong

doi:10.1039/c3nr01464a

Cited by 7 publications

(3 citation statements)

References 25 publications

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“…In this study, it took 3 min to obtain one frame. With the high-speed AFM technology [59][60][61][62][63] , which is capable of imaging at 50-100 ms per frame under physiological conditions, more detailed dynamic observation could be possible.…”

Section: Resultsmentioning

confidence: 99%

Direct visualization of the extracellular binding structure of E-cadherins in liquid

Shibata-Seki

Nagaoka

Goto

et al. 2020

Sci Rep

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E-cadherin is a key Ca-dependent cell adhesion molecule, which is expressed on many cell surfaces and involved in cell morphogenesis, embryonic development, EMT, etc. The fusion protein E-cad-Fc consists of the extracellular domain of E-cadherin and the IgG Fc domain. On plates coated with this chimeric protein, ES/iPS cells are cultivated particularly well and induced to differentiate. The cells adhere to the plate via E-cad-Fc in the presence of Ca2+ and detach by a chelating agent. For the purpose of clarifying the structures of E-cad-Fc in the presence and absence of Ca2+, we analyzed the molecular structure of E-cad-Fc by AFM in liquid. Our AFM observations revealed a rod-like structure of the entire extracellular domain of E-cad-Fc in the presence of Ca2+ as well as trans-binding of E-cad-Fc with adjacent molecules, which may be the first, direct confirmation of trans-dimerization of E-cadherin. The observed structures were in good agreement with an X-ray crystallographic model. Furthermore, we succeeded in visualizing the changes in the rod-like structure of the EC domains with and without calcium. The biomatrix surface plays an important role in cell culture, so the analysis of its structure and function may help promote cell engineering based on cell recognition.

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Section: Resultsmentioning

confidence: 99%

Direct visualization of the extracellular binding structure of E-cadherins in liquid

Shibata-Seki

Nagaoka

Goto

et al. 2020

Sci Rep

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“…To enlarge the scan range, we built a fast AFM, where the X-Y motion is handled by a flexure stage that moves the sample while the Z motion is handled by a piezo stack moving the cantilever up and down. This homemade fast AFM has up to one hundred micrometers scan range and up to 50Hz line scan rate [6]. When in test processing, we found that with the most popular controller in AFM fast scanning, such as dynamic PI controller [7], model-based feedback controller [8] and inversion-based iterative controller [9], the Z scanner vibration can not be eliminated ideally.…”

Section: Fig1 Schematic Diagram Of Afm Operation[4]mentioning

confidence: 99%

Active Disturbance Rejection Control Design for Fast AFM

Chen¹,

Yin²,

Liu³

et al. 2015

KEM

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Active disturbance rejection control (ADRC) as an alternative to classical PID control to solving control problems, has gained significant traction these years. With its simple tuning method, robustness against process parameter variations and ability of disturbance rejection, we tried it in our homemade fast atomic force microscope (AFM). Experiments are carried out in contact mode on standard optical grating sample with 50Hz line rate. The results show that ADRC can reject external disturbances well, and can reduce system vibrations obviously.

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“…To maintain controlled scanner motion at high scan rates, the design and control of mechanical scanners has played a crucial role in the development of HS-AFM systems [26,27]. Small, stiff scanners based on piezo tubes [2,3] or flexures optimized using finite element methods [4,6,18,28,[29][30][31] allow control to be maintained at high frequencies, but often at the expense of maximum scan size [32][33][34]. Notch filtering and model based feedback have allowed scanners to be driven at higher frequencies while mitigating the effects of resonant behavior [26,27,35,38,39], and non-raster scanning techniques such as Lissajous [40] and spiral scanning [41][42][43] allow control at high scan rates as the scanner drive signals do not contain components at higher harmonics of the scan frequency.…”

Section: Introductionmentioning

confidence: 99%

High-speed large area atomic force microscopy using a quartz resonator

Wang

Mullin²,

Hobbs

2018

Nanotechnology

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A high-speed atomic force microscope for scanning large areas, utilizing a quartz bar driven close to resonance to provide the motion in the fast scan axis is presented. Images up to 170 × 170 μm have been obtained on a polydimethylsiloxane (PDMS) grating in 1 s. This is provided through an average tip-sample velocity of 28 cm s at a line rate of 830 Hz. Scan areas up to 80 × 80 μm have been obtained in 0.42 s with a line rate of 1410 Hz. To demonstrate the capability of the scanner the spherulitic crystallization of a semicrystalline polymer was imaged in situ at high speed.

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High-speed AFM for scanning the architecture of living cells

Cited by 7 publications

References 25 publications

Direct visualization of the extracellular binding structure of E-cadherins in liquid

Direct visualization of the extracellular binding structure of E-cadherins in liquid

Active Disturbance Rejection Control Design for Fast AFM

High-speed large area atomic force microscopy using a quartz resonator

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