Hanne Roberg-Larsen, Martin Frank Strand, Anders Grimsmo, Petter Angell Olsen, Jennifer L. Dembinski, Frode Rise, Elsa Lundanes, Tyge Greibrokk, Stefan Krauss, Steven Ray Wilson
Abstract:Oxysterols are important in numerous biological processes, including cell signaling. Here we present an automated filtration/filter backflush-solid phase extraction-liquid chromatography-tandem mass spectrometry (AFFL-SPE-LC-MS/MS) method for determining 24-hydroxysterol and the isomers 25-hydroxycholesterol and 22S-hydroxycholesterol that enables simplified sample preparation, high sensitivity (~25 pg/mL cell lysis sample) and low sample variability. Only one sample transfer step was required for the entire p…
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