High sensitive and broad-range detection of cortisol in human saliva using a trap lateral flow immunoassay (trapLFI) sensor

Oh, Hyun-Kyung; Kim, Ju‐Wan; Kim, Jae-Min; Kim, Min-Gon

doi:10.1039/c8an00719e

Cited by 34 publications

(27 citation statements)

References 17 publications

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“…As aforementioned, LFAs are used in a variety of applications with samples that present extremely different composition and characteristics (Table 1). 27 For example, clinical samples can be whole blood, 28,29 plasma, 30,31 serum, [32][33][34] sweat, 35,36 urine, 37,38 stool, 39,40 saliva, 41,42 cerebrospinal fluid 43,44 and nasal swabs, 45,46 while food matrices could be juices, 47,48 cereals, 49,50 meat, 51,52 vegetables, 53 and environmental (mostly water and soil) samples. [54][55][56][57] Although the sample pad provides a means to control the properties of the sample solution (see the following section), some complex matrixes may require pre-treatment before an aliquot can be added into the LFA strip.…”

Section: Types Of Samples and Target Analytesmentioning

confidence: 99%

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

et al. 2020

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Lateral flow assays (LFA) are quick, simple and cheap assays to analyse a variety of samples at the point of care or in the field, making them one of the most widespread biosensors currently available. They have been successfully employed for the detection of a myriad of different targets (ranging from atoms up to whole cells) in all type of samples (including water, blood, foodstuff and environmental samples). Their operation relies on the capillary flow of the sample within a series of sequential pads with different functionalities aiming to generate a signal indicating the absence/presence (and, in some cases, the concentration) of the analyte of interest. In order to have a user-friendly operation, their development requires the optimization of multiple, interconnected parameters that may overwhelm new developers. In this Tutorial we provide the readers with: 1) the basic knowledge to understand the principles governing an LFA and to take informed decisions during lateral flow strip design and fabrication, 2) a roadmap for optimal LFA development independent of the specific application, 3) a step by step example protocol for the assembly and operation of an LF strip for the detection of Human Immunoglobulin G and 4) an extensive troubleshooting section addressing the most frequent issues in designing, assembling and using LFAs.

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Section: Types Of Samples and Target Analytesmentioning

confidence: 99%

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

et al. 2020

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“…By tuning the concentration of cortisol-BSA conjugates in the deletion zone, and applying a ratiometric imaging method, the authors reported a 30-fold improvement over a conventional cortisol LFA. 75 Limitations. The reaction/transport category is home to efforts that use porous materials to automate new reaction schemes with unconventional transport dynamics.…”

Section: Reaction/transportmentioning

confidence: 99%

Sensitivity enhancement in lateral flow assays: a systems perspective

et al. 2019

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“…Several examples were reported based on the use of chromogenic substrates for enhancing colorimetric-based LFIAs [ 52 , 53 ]. Competitive LFIAs for multiplexed analysis of carbaryl and endosulfan using AuNPs and HRP as labels were developed by Zhang et al [ 54 ] to compare the performances obtained with the different systems.…”

Section: Enzyme-labeled Conjugates In Lfiamentioning

confidence: 99%

“…Using this platform, the HRP color signals increase in the detection zone and decrease in the deletion zone depending on the concentration of the target analyte. The ratio of signals from deletion and detection zones allowed to quantify cortisol in a wide concentration range (0.01–100 ng mL −1 ) with high detection sensitivity (9.9 pg mL −1 ) [ 52 ].…”

Section: Enzyme-labeled Conjugates In Lfiamentioning

confidence: 99%

Recent Advancements in Enzyme-Based Lateral Flow Immunoassays

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Zangheri

et al. 2021

Sensors

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Paper-based lateral-flow immunoassays (LFIAs) have achieved considerable commercial success and their impact in diagnostics is continuously growing. LFIA results are often obtained by visualizing by the naked eye color changes in given areas, providing a qualitative information about the presence/absence of the target analyte in the sample. However, this platform has the potential to provide ultrasensitive quantitative analysis for several applications. Indeed, LFIA is based on well-established immunological techniques, which have known in the last year great advances due to the combination of highly sensitive tracers, innovative signal amplification strategies and last-generation instrumental detectors. All these available progresses can be applied also to the LFIA platform by adapting them to a portable and miniaturized format. This possibility opens countless strategies for definitively turning the LFIA technique into an ultrasensitive quantitative method. Among the different proposals for achieving this goal, the use of enzyme-based immunoassay is very well known and widespread for routine analysis and it can represent a valid approach for improving LFIA performances. Several examples have been recently reported in literature exploiting enzymes properties and features for obtaining significative advances in this field. In this review, we aim to provide a critical overview of the recent progresses in highly sensitive LFIA detection technologies, involving the exploitation of enzyme-based amplification strategies. The features and applications of the technologies, along with future developments and challenges, are also discussed.

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High sensitive and broad-range detection of cortisol in human saliva using a trap lateral flow immunoassay (trapLFI) sensor

Abstract: Cortisol, a steroid hormone, is a main biomarker of psychological stress.

Cited by 34 publications

References 17 publications

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

Sensitivity enhancement in lateral flow assays: a systems perspective

Recent Advancements in Enzyme-Based Lateral Flow Immunoassays

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