High-resolution nuclear magnetic resonance spectroscopy studies of polysaccharides crosslinked by sodium trimetaphosphate: a proposal for the reaction mechanism

Lack, Stéphane; Dulong, Virginie; Picton, Luc; Cerf, Didier Le; Condamine, Eric

doi:10.1016/j.carres.2007.01.011

Cited by 96 publications

(74 citation statements)

References 18 publications

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“…This 3-D structure was prepared by cross-linking the biopolymers in the absence of organic solvents. A cross-linking process carried out in aqueous conditions provided a simple method of obtaining a polysaccharide-based scaffold [65]. Investigations combining cross-linking with a porogen agent and a salt-leaching technique allowed preparation of porous scaffolds of controlled porosity that could be colonized by various cell types, including MSCs [50,51].…”

Section: Discussionmentioning

confidence: 99%

Cell interactions between human progenitor-derived endothelial cells and human mesenchymal stem cells in a three-dimensional macroporous polysaccharide-based scaffold promote osteogenesis

Guerrero¹,

Catros²,

Derkaoui³

et al. 2013

Acta Biomaterialia

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Section: Discussionmentioning

confidence: 99%

Cell interactions between human progenitor-derived endothelial cells and human mesenchymal stem cells in a three-dimensional macroporous polysaccharide-based scaffold promote osteogenesis

Guerrero¹,

Catros²,

Derkaoui³

et al. 2013

Acta Biomaterialia

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“…[28][29] The new process by crosslinking with STMP and progressive evaporation, described here for the first time, constitutes an original procedure that endows the PVA with very particular features. The mechanisms of the STMP cross-linking reaction for hydroxylated polymers, such as polysaccharides, have been recently described by Lack et al [30] Phosphoesterification of hydroxyl residues is the main reaction, leading to the formation of phosphoester bridges and phosphate hanging groups, which are negatively charged, favoring a high hydration. PVA has a chemical structure close to that of polysaccharides.…”

Section: Discussionmentioning

confidence: 99%

A Novel Cross‐linked Poly(vinyl alcohol) (PVA) for Vascular Grafts

Chaouat

Visage

Baille

et al. 2008

Adv Funct Materials

171

159

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Poly(vinyl alcohol) (PVA) is a water‐soluble synthetic polymer with excellent film‐forming, emulsifying, and adhesive properties. The aim of this study is to design a simple process for PVA cross‐linking with sodium trimetaphosphate to form membrane devices suitable for biomedical applications. This procedure requires no organic solvent, nor melting process to obtain films with high mechanical strength. Fabrication of a small diameter tube from a PVA film is easy with a single wrapping step around a Teflon rod. Dynamic mechanical analysis demonstrated that, upon removal of the applied stress, the PVA film with a Young's modulus of 2 × 105 kPa returns to its original size and shape. The wall thickness of PVA tubes is 344 ± 13 µm (n = 12), which is close to the wall thickness of a human artery (350–710 µm). Suture retention of a PVA tube is excellent (140 ± 11 g), close to that of human vessels. The burst pressure of PVA tubes is found to be 507 ± 25 mm Hg, more than three times higher than the human healthy systolic arterial pressure. Under arterial pressure, there was no leakage even after needle puncture, contrary to clinical vascular expanded polytetrafluoroethylene prostheses. Finally, PVA tubes of 2 mm in diameter are used to replace a segment of an infrarenal aorta in rats. For at least one week, no mechanical nor thrombotic complications are noticed even in the absence of anticoagulant or antiplatelet treatment. Graft patency is also evidenced with non‐invasive imaging techniques. As a conclusion, this novel cross‐linking method confers to poly(vinyl alcohol) particular mechanical properties such as compliance, elasticity and resistance to mechanical stress, compatible with the circulatory blood flow.

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“…On the contrary, at higher concentrations of the polymer the binding between different chains is favoured with the release of pyrophosphate affording the formation of a 3D scaffold. [9] IR analysis of another at 990 cm À1 relative to the O-P-C stretching ( Figure 1). A similar spectrum with the appearance of the same three bands was recorded for CMCAP.…”

Section: Resultsmentioning

confidence: 99%

A new Phosphorylated Polysaccharide for Biomedical Applications: Generation of 3D Scaffolds with Osteogenic Activity and Coating of Titanium Oxide Surfaces

Pasqui

Danieli²,

Leone

et al. 2008

Macromolecular Symposia

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A new phosphorylated derivative of carboxymethylcellulose and amidic carboxymethylcellulose containing one phosphate group for each disaccharide unit was synthesized using sodium trimetaphospahte (STMP) as the phosphating agent. The new polysaccharide was characterized by infrared spectroscopy (FT-IR) and the amount of phosphate groups was determined by elemental analysis. These modified polysaccharides were used both to prepare 3D scaffolds and functionalize titanium oxide surfaces with the aim to improve the osseointegration with the host tissue. The presence of phosphate groups modify the physical-chemical properties of the hydrogels with respect to the native ones. The evaluation of the bioactivity of the phosphorylated carboxymethylcellulose hydrogels towards osteoblast-like cells showed a significant increase in the osteocalcin production. The modified surfaces were chemically characterized by means of X-ray photoelectron spectroscopy (XPS) and FT-IR, whereas the surface topography was analysed by Atomic Force Measurements (AFM) measurements before and after the polysaccharide coating. In vitro biological tests using osteoblast-like cells demonstrated that phosphorylated carboxymethylcellulose functionalized TiO2 surfaces promoted better cell adhesion and significantly enhanced their proliferation. These findings suggest that the phosphate polysaccharide both as a 3D scaffold and as a surface coating promotes osteoblast growth potentially improving the biomaterial osseointegration rate

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High-resolution nuclear magnetic resonance spectroscopy studies of polysaccharides crosslinked by sodium trimetaphosphate: a proposal for the reaction mechanism

Cited by 96 publications

References 18 publications

Cell interactions between human progenitor-derived endothelial cells and human mesenchymal stem cells in a three-dimensional macroporous polysaccharide-based scaffold promote osteogenesis

Cell interactions between human progenitor-derived endothelial cells and human mesenchymal stem cells in a three-dimensional macroporous polysaccharide-based scaffold promote osteogenesis

A Novel Cross‐linked Poly(vinyl alcohol) (PVA) for Vascular Grafts

A new Phosphorylated Polysaccharide for Biomedical Applications: Generation of 3D Scaffolds with Osteogenic Activity and Coating of Titanium Oxide Surfaces

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