“…This method is considered fast, sensitive, and low-cost in comparison to traditional assays such as single strand conformation polymorphism (SSCP), denaturing gradient gel electrophoresis (DGGE), and denaturing high-performance liquid chromatography (DHPLC) (Hofinger et al, 2009). Even though HRM analysis has been widely used in clinical applications, it was recently adapted for genotyping in cereal species, including barley (Lehmensiek et al, 2008;Hofinger et al, 2009), wheat (Botticella et al, 2011;Sestili et al, 2015), and rice (Li et al, 2011). Allelic variants of eukaryotic translation initiation 4E (HvelF4E) gene have been investigated in barley with HRM analysis for the potential application in targeting induced local lesions in genomes (TILLING) (McCallum et al, 2000;Hofinger et al, 2009).…”