2011
DOI: 10.1186/1471-2229-11-156
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Abstract: BackgroundManipulation of the amylose-amylopectin ratio in cereal starch has been identified as a major target for the production of starches with novel functional properties. In wheat, silencing of starch branching enzyme genes by a transgenic approach reportedly caused an increase of amylose content up to 70% of total starch, exhibiting novel and interesting nutritional characteristics.In this work, the functionality of starch branching enzyme IIa (SBEIIa) has been targeted in bread wheat by TILLING. An EMS-… Show more

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Cited by 115 publications
(102 citation statements)
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“…Moreover, the variability can be also reduced by using a nested (two-round) PCR for amplicon production. This is often an essential step in polyploid species as there may be insufficient polymorphism between homoeologues to generate specific primers (Botticella et al 2011). 2.…”
Section: Notesmentioning
confidence: 99%
See 1 more Smart Citation
“…Moreover, the variability can be also reduced by using a nested (two-round) PCR for amplicon production. This is often an essential step in polyploid species as there may be insufficient polymorphism between homoeologues to generate specific primers (Botticella et al 2011). 2.…”
Section: Notesmentioning
confidence: 99%
“…BRCA1 and BRCA2 genes, Hondow et al 2011). This method has also been used to detect mutations (single-nucleotide polymorphisms, SNPs and small insertions/deletions, InDels) in plant genetic studies, including TILLING in Lycopersicon esculentum (Gady et al 2009), Oryzias latipes (Ishikawa et al 2010), Triticum aestivum (Botticella et al 2011), Brassica rapa (Lochlainn et al 2011) and genetic mapping in Hordeum vulgare (Lehmensiek et al 2008), Zea mays (Li et al 2010) and Oryza sativa (Li et al 2011). HRM has also been used for the identification of transgenic plants (Milner et al 2014) and the detection of DNA methylation (Wojdacz and Dobrovic 2007).…”
Section: Introductionmentioning
confidence: 99%
“…This method is considered fast, sensitive, and low-cost in comparison to traditional assays such as single strand conformation polymorphism (SSCP), denaturing gradient gel electrophoresis (DGGE), and denaturing high-performance liquid chromatography (DHPLC) (Hofinger et al, 2009). Even though HRM analysis has been widely used in clinical applications, it was recently adapted for genotyping in cereal species, including barley (Lehmensiek et al, 2008;Hofinger et al, 2009), wheat (Botticella et al, 2011;Sestili et al, 2015), and rice (Li et al, 2011). Allelic variants of eukaryotic translation initiation 4E (HvelF4E) gene have been investigated in barley with HRM analysis for the potential application in targeting induced local lesions in genomes (TILLING) (McCallum et al, 2000;Hofinger et al, 2009).…”
Section: Introductionmentioning
confidence: 99%
“…This technique has been successfully applied to EMS-induced mutant screening by targeting induced local lesions in genomes in common wheat (Dong et al, 2009;Botticella et al, 2011). Therefore, we considered that HRM analysis might help efficiently detect SNPs and construct high-density maps of the wheat genome.…”
mentioning
confidence: 99%