High Primary Antibiotic Resistance of Helicobacter pylori Strains Isolated from Pediatric and Adult Patients in Poland during 2016–2018

Krzyżek, Paweł; Pawełka, Dorota; Iwańczak, Barbara; Kempiński, Radosław; K, Leśniakowski; Mégraud, Françis; Łaczmański, Łukasz; Biernat, Monika; Gościniak, Grażyna

doi:10.3390/antibiotics9050228

Cited by 11 publications

(13 citation statements)

References 34 publications

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“…The latest data on H. pylori antibiotic resistance in Europe showed that the level of primary resistance of this bacterium was 21.8% for clarithromycin (CLR), 15.8% for levofloxacin (LEV), and 38.9% for metronidazole (MTZ) [ 5 ]. The results of this European study are consistent with both world-level [ 6 ] and national-level data published by our team [ 7 ]. Therefore, attention should be paid to the maintenance of an appropriate degree of H. pylori eradication and development of alternative, effective methods for combating this pathogen [ 8 , 9 , 10 ].…”

Section: Introductionsupporting

confidence: 92%

“…Synergistic approaches aimed at treating infections caused by H. pylori are currently of high hopes (a literature review by Krzyżek et al (2020) [33]). Using a checkerboard assay, we determined the existence of interactions between extracts (A4/2 and A5/1) and synthetic substances with proven antibacterial activity against H. pylori, i.e., the routinely used antibiotic AMX, as well as sertraline (SER) and 3-bromopyruvate (3-BP), which we have recently studied for their anti-H. pylori properties [22,34,35].…”

Section: Synergistic Activity Of Extracts and Synthetic Drugsmentioning

confidence: 99%

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Antibiofilm and Antimicrobial-Enhancing Activity of Chelidonium majus and Corydalis cheilanthifolia Extracts against Multidrug-Resistant Helicobacter pylori

et al. 2021

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Helicobacter pylori is a Gram-negative bacterium that colonizes the stomach of about 60% of people worldwide. The search for new drugs with activity against H. pylori is now a hotspot in the effective and safe control of this bacterium. Therefore, the aim of this research was to determine the antibacterial activity of extracts from selected plants of the Papaveraceae family against planktonic and biofilm forms of the multidrug-resistant clinical strain of H. pylori using a broad spectrum of analytical in vitro methods. It was revealed that among the tested extracts, those obtained from Corydalis cheilanthifolia and Chelidonium majus were the most active, with minimal inhibitory concentrations (MICs) of 64 µg/mL and 128 µg/mL, respectively. High concentrations of both extracts showed cytotoxicity against cell lines of human hepatic origin. Therefore, we attempted to lower their MICs through the use of a synergistic combination with synthetic antimicrobials as well as by applying cellulose as a drug carrier. Using checkerboard assays, we determined that both extracts presented synergistic interactions with amoxicillin (AMX) and 3-bromopyruvate (3-BP) (FICI = 0.5) and additive relationships with sertraline (SER) (FICI = 0.75). The antibiofilm activity of extracts and their combinations with AMX, 3-BP, or SER, was analyzed by two methods, i.e., the microcapillary overgrowth under flow conditions (the Bioflux system) and assessment of the viability of lawn biofilms after exposure to drugs released from bacterial cellulose (BC) carriers. Using both methods, we observed a several-fold decrease in the level of H. pylori biofilm, indicating the ability of the tested compounds to eradicate the microbial biofilm. The obtained results indicate that application of plant-derived extracts from the Papaveraceae family combined with synthetic antimicrobials, absorbed into organic BC carrier, may be considered a promising way of fighting biofilm-forming H. pylori.

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Section: Introductionsupporting

confidence: 92%

Section: Synergistic Activity Of Extracts and Synthetic Drugsmentioning

confidence: 99%

Antibiofilm and Antimicrobial-Enhancing Activity of Chelidonium majus and Corydalis cheilanthifolia Extracts against Multidrug-Resistant Helicobacter pylori

et al. 2021

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“…Dual resistance to clarithromycin and metronidazole of H. pylori is considered to be the most important resistance profile determining therapeutic failures of this pathogen [ 33 , 34 ], therefore we extended our research with three clinical H. pylori strains with the above-mentioned resistance. Checkerboard assays determining the sub-MICs of MYR with clarithromycin or metronidazole confirmed our previously described observations, indicating synergism of 1/4× MIC of MYR with these antibiotics (FICI = 0.31–0.5) and suggesting that resistance to antibiotics of H. pylori is not adversely affecting the antibiotic-boosting effect of MYR ( Figure 6 ).…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, three clinical strains of H. pylori (M26, M91, and M145), having dual antibiotic resistance against clarithromycin and metronidazole, were also utilized during the checkerboard assays. These strains come from the collection of microorganisms of the Wrocław Medical University in Poland and were isolated during previous studies [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

Myricetin as an Antivirulence Compound Interfering with a Morphological Transformation into Coccoid Forms and Potentiating Activity of Antibiotics against Helicobacter pylori

Krzyżek

Migdał

Paluch

et al. 2021

IJMS

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Helicobacter pylori, a gastric pathogen associated with a broad range of stomach diseases, has a high tendency to become resistant to antibiotics. One of the most important factors related to therapeutic failures is its ability to change from a spiral to a coccoid form. Therefore, the main aim of our original article was to determine the influence of myricetin, a natural compound with an antivirulence action, on the morphological transformation of H. pylori and check the potential of myricetin to increase the activity of antibiotics against this pathogen. We observed that sub-minimal inhibitory concentrations (sub-MICs) of this compound have the ability to slow down the process of transformation into coccoid forms and reduce biofilm formation of this bacterium. Using checkerboard assays, we noticed that the exposure of H. pylori to sub-MICs of myricetin enabled a 4–16-fold reduction in MICs of all classically used antibiotics (amoxicillin, clarithromycin, tetracycline, metronidazole, and levofloxacin). Additionally, RT-qPCR studies of genes related to the H. pylori morphogenesis showed a decrease in their expression during exposure to myricetin. This inhibitory effect was more strongly seen for genes involved in the muropeptide monomers shortening (csd3, csd6, csd4, and amiA), suggesting their significant participation in the spiral-to-coccoid transition. To our knowledge, this is the first research showing the ability of any compound to synergistically interact with all five antibiotics against H. pylori and the first one showing the capacity of a natural substance to interfere with the morphological transition of H. pylori from spiral to coccoid forms.

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“…An emerging problem in all regions of the world is the increasing H. pylori antibiotic resistance, particularly to clarithromycin. This means that clarithromycin-based treatment cannot be considered without a further testing regime to see whether the organisms are sensitive [ 66 , 67 ]. Resistance of H. pylori strains to various antibiotics, mainly clarithromycin and levofloxacin, may be the one of the reasons for treatment failure.…”

Section: Role Of Bacteria In Gastrointestinal Lymphomamentioning

confidence: 99%

Bacterial Infection and Non-Hodgkin B-Cell Lymphoma: Interactions between Pathogen, Host and the Tumor Environment

Biernat

Wróbel

2021

IJMS

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Non-Hodgkin B-cell lymphomas (NHL) are a heterogeneous group of lymphoid neoplasms with complex etiopathology, rich symptomatology, and a variety of clinical courses, therefore requiring different therapeutic approaches. The hypothesis that an infectious agent may initiate chronic inflammation and facilitate B lymphocyte transformation and lymphogenesis has been raised in recent years. Viruses, like EBV, HTLV-1, HIV, HCV and parasites, like Plasmodium falciparum, have been linked to the development of lymphomas. The association of chronic Helicobacter pylori (H. pylori) infection with mucosa-associated lymphoid tissue (MALT) lymphoma, Borrelia burgdorferi with cutaneous MALT lymphoma and Chlamydophila psittaci with ocular adnexal MALT lymphoma is well documented. Recent studies have indicated that other infectious agents may also be relevant in B-cell lymphogenesis such as Coxiella burnettii, Campylobacter jejuni, Achromobacter xylosoxidans, and Escherichia coli. The aim of the present review is to provide a summary of the current literature on infectious bacterial agents associated with B-cell NHL and to discuss its role in lymphogenesis, taking into account the interaction between infectious agents, host factors, and the tumor environment.

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High Primary Antibiotic Resistance of Helicobacter pylori Strains Isolated from Pediatric and Adult Patients in Poland during 2016–2018

Cited by 11 publications

References 34 publications

Antibiofilm and Antimicrobial-Enhancing Activity of Chelidonium majus and Corydalis cheilanthifolia Extracts against Multidrug-Resistant Helicobacter pylori

Antibiofilm and Antimicrobial-Enhancing Activity of Chelidonium majus and Corydalis cheilanthifolia Extracts against Multidrug-Resistant Helicobacter pylori

Myricetin as an Antivirulence Compound Interfering with a Morphological Transformation into Coccoid Forms and Potentiating Activity of Antibiotics against Helicobacter pylori

Bacterial Infection and Non-Hodgkin B-Cell Lymphoma: Interactions between Pathogen, Host and the Tumor Environment

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