High prevalence of human papillomavirus type 58 in Chinese women with cervical cancer and precancerous lesions

Abstract

The prevalence of human papillomavirus (HPV) among 332 Hong Kong Chinese women with abnormal Papanicolaou smears were determined by polymerase chain reaction and restriction fragment length polymorphism analysis. The overall HPV positive rate was 44.3% with 18.6% (16/86) for normal/inflamed cervices, 36.4% (32/88) for condyloma, 64.7% (33/51) for cervical intraepithelial neoplasia grade 1 (CIN 1), 37.9% (11/29) for CIN 2, 68.3 (41/60) for CIN 3, and 77.8% (14/18) for carcinoma. Double HPV infection was detected in 17 of the 147 positive samples, with a significantly higher proportion in patients with normal or inflamed cervices than those with CIN or carcinoma (31.3% vs 10.5%, P =.029). The six most commonly identified genotypes were HPV 16 (33.3%), HPV 58 (23.8%), HPV 11, 18, 31 (8.8% each), and HPV 33 (6.8%). The worldwide uncommon genotype HPV 58 was found to be the second most common genotype detected in patients with cervical carcinoma (6 of 18 patients). HPV 58 infection showed a significant association with CIN/carcinoma (odds ratio [OR] = 3.98; 95% confidence interval [CI] = 1.22-14.35) and a significant trend of increase in prevalence with increasing severity of cervical lesion (chi(2) = 5.84; P =.016). Among Hong Kong Chinese women with abnormal cervical cytology, the detection of HPV 58 carried a positive predictive value of 68.6% for a cervical lesion of CIN 1 or higher severity. The high prevalence of HPV 58 among Chinese women, particularly in patients with carcinoma, has an implication on the design of HPV detection methods and the development of vaccines.

Type‐specific human papillomavirus oncogene messenger RNA levels correlate with the severity of cervical neoplasia

“…[8][9][10][11][12][13][14][15]36,37 The overall presence of HPV 16, 18, 52, 58, 31 and 33 E6 mRNA transcripts (68.7%) in the 6 types of HPV DNA-positive cases in CIN lesions is higher than that in a previous study (46%). 21 The presence of these 6 types mRNA transcripts with HPV DNA positive is 80% for CIN 3 but only 13% for normal cytology.…”

“…1 In addition, HPV 52 and 58 are more prevalent in patients with preinvasive and cervical cancer in Taiwan and other Asian countries. [8][9][10][11][12][13][14][15] On the other hand, the occurrence of infection with HPV types 52 or 58 is relatively uncommon in cervical cancer in the Americas, Europe, Africa and …”

Distribution of human papillomavirus types in cervical cancers in Hong Kong: Current situation and changes over the last decades

“…Although numerous test kits with high sensitivity and covering a broad spectrum of HPV types are now commercially available, the interpretation of these results is not straight forward. By using these methods, coinfections with multiple HPV types are not only common for normal and low-grade lesions, [21][22][23][24][25] but also found in a notable, although smaller proportion of invasive cancers. [26][27][28][29][30][31] The current understanding favours the notion that only one of the coinfecting types is responsible for cancer development.…”

Biases in human papillomavirus genotype prevalence assessment associatedwith commonly used consensus primers

“…7,8 HPV type was identified by restriction fragment length polymorphisms (RFLPs) using endonucleases RsaI and DdeI as previously described. 8 Ambiguous RFLPs were resolved by direct sequencing of PCR amplicons. The third method was based on the premarket version of the HPV Consensus PCR/Line Blot Genotyping Kit (Roche Molecular Systems, Alameda, CA).…”