High prevalence of blaOXA-23 in Acinetobacter spp. and detection of blaNDM-1 in A. soli in Cuba: report from National Surveillance Program (2010–2012)

Abstract

As a first national surveillance of Acinetobacter in Cuba, a total of 500 Acinetobacter spp. isolates recovered from 30 hospitals between 2010 and 2012 were studied. Acinetobacter baumannii–calcoaceticus complex accounted for 96.4% of all the Acinetobacter isolates, while other species were detected at low frequency (A. junii 1.6%, A. lwoffii 1%, A. haemolyticus 0.8%, A. soli 0.2%). Resistance rates of isolates were 34–61% to third-generation cephalosporins, 49–50% to β-lactams/inhibitor combinations, 42–47% to aminoglycosides, 42–44% to carbapenems and 55% to ciprofloxacin. However, resistance rates to colistin, doxycycline, tetracycline and rifampin were less than 5%. Among carbapenem-resistant isolates, 75% harboured different blaOXA genes (OXA-23, 73%; OXA-24, 18%; OXA-58, 3%). The blaNDM-1 gene was identified in an A. soli strain, of which the species was confirmed by sequence analysis of 16S rRNA gene, rpoB, rpoB–rpoC and rpoL–rpoB intergenic spacer regions and gyrB. The sequences of blaNDM-1 and its surrounding genes were identical to those reported for plasmids of A. baumannii and A. lwoffi strains. This is the first report of blaNDM-1 in A. soli, together with a high prevalence of OXA-23 carbapenemase for carbapenem resistance in Acinetobacter spp. in Cuba.

Isolation, Characterization of Acinetobacter Baumannii Resistant to Carbapenem and Their Effect on Mice Hepatorenal Functions.

“…We obtained 32 (72.72%) isolates carried bla OXA-23 and 13 (29.54%) isolates carried bla OXA-58. .These results were in agreement with Quiñones et al, (2015) (35) who found that, from 30 A.baumannii strains 76% were positive for bla OXA-23 gene and 3% were positive for bla OXA-58 by multiplex PCR, on the other hand, Shoja et al, (2013) (36) detected bla OXA-23 in 85% of isolates but the bla OXA-58 was not found, resistance to carbapenems in these isolates may be related to other mechanisms including decreased permeability, alteration of penicillin-binding proteins, AmpC stable derepression and overexpression of efflux pump (37) .…”

High Prevalence of CTX-M Type Extended-Spectrum Beta-Lactamase Genes and Detection of NDM-1 Carbapenemase Gene in Extraintestinal Pathogenic Escherichia coli in Cuba

“…In our present study, NDM-1 gene was identified in two ExPEC isolates of phylogenetic group B1-ST448 that were isolated from sporadic infections in 2016, representing NDM-detection rate of 0.7% (2/306). This is the first report of NDM-1 gene in E. coli in Cuba, although we identified bla NDM-1 in a rare Acinetobacter species, A. soli in 2011 [27]. It was also remarkable in the present study that bla NDM-1 was detected in a rare E. coli clone, ST448.…”

Genomic Epidemiology of NDM-1-Encoding Plasmids in Latin American Clinical Isolates Reveals Insights into the Evolution of Multidrug Resistance

“…Acinetobacter spp. harboring bla NDM-1 in pNDM-BJ01-like plasmids are frequently detected all over the world ( Feng et al 2015 ; Fu et al 2015 ); these (or NDM-positive isolates with bla NDM-1 genetic surroundings suggesting the presence of pNDM-BJ01-like plasmids) have even been found in Latin America ( Waterman et al 2013 ; Pasteran et al 2014 ; Brovedan et al 2015 ; Quinones et al 2015 ; Montana et al 2016 ; Rojas et al 2016 ). Here, we report an A. baumannii clone isolated in Colombia that also harbors bla NDM-1 in a pNDM-BJ01-like plasmid.…”