High-performance reverse phase chromatography with fluorescence detection assay for characterization and quantification of pneumococcal polysaccharides

Canaán-Haden, Leonardo; Cremata, José A.; Chang, Janoi; Valdés, Yolanda; Cardoso, Félix; Bencomo, Vicente Vérez

doi:10.1016/j.vaccine.2005.01.128

Cited by 8 publications

(1 citation statement)

References 2 publications

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“…This problem is exacerbated for multiresidue pneumococcal polysaccharides that contain both acid-resistant (e.g., uronic acids, amino sugars) and acid-labile keto and deoxy sugar monomers. Two approaches for the composition analysis of pneumococcal vaccines that use derivatization are: reversed phase HPLC of fluorophore-labeled sugars (Canaán-Haden et al 2006) and gas chromatography with mass spectrometric detection (GC-MS) performed after methanolysis, re-N-acetylation and trimethylsilylation . Liquid chromatography (LC) permits direct analysis of the analytes, whereas gas-liquid chromatography (GC) and some LC methods require prior derivatization.…”

Section: Polysaccharide Composition Analysismentioning

confidence: 99%

Vaccine Analysis: Strategies, Principles, and Control

Nunnally¹,

Turula²,

Sitrin³

2015

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Section: Polysaccharide Composition Analysismentioning

confidence: 99%

Vaccine Analysis: Strategies, Principles, and Control

Nunnally¹,

Turula²,

Sitrin³

2015

View full text Add to dashboard Cite

The Pre‐ and Post‐Column Derivatization on Monosaccharide Composition Analysis, a Review

Chen,

Wang,

et al. 2024

Chemistry & Biodiversity

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Polysaccharides, as common metabolic products in organisms, play a crucial role in the growth and development of living organisms. For humans, polysaccharides represent a class of compounds with diverse applications, particularly in the medical field. Therefore, the exploration of the monosaccharide composition and structural characteristics of polysaccharides holds significant importance in understanding their biological functions. This review provides a comprehensive overview of extraction methods and hydrolysis strategies for polysaccharides. It systematically analyzes strategies and technologies for determining polysaccharide composition and discusses common derivatization reagents employed in further polysaccharide studies. Derivatization is considered a fundamental strategy for determining monosaccharides, as it not only enhances the detectability of analytes but also increases detection sensitivity, especially in liquid chromatography (LC), capillary electrophoresis (CE), and gas chromatography (GC) techniques. The critical comparison of pre‐ and post‐column derivatization is elaborated in this paper, aiming to serve as a reference for selecting appropriate modes based on the structural characteristics, biological activities of polysaccharides, and reaction systems.

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