High-Performance Liquid Chromatography versus Other Modern Analytical Methods for Determination of Pesticides

Review of advances in the thin layer chromatography of pesticides: 2012–2014

“…[9] Modern TLC complements gas chromatography (GC) and high-performance liquid chromatography (HPLC) for pesticide separation, detection, identification, and quantification because of unique characteristics relative to column chromatography. A new book published in Chromatographic Science Series [10] described TLC as a pilot method for HPLC, TLC coupled with a diode array detector (DAD), TLC coupled with mass spectrometry (MS), TLC coupled with nuclear magnetic resonance (NMR) and Fourier transform infrared (FTIR) spectrometry, multiple development modes of separation, and multidimensional TLC in pesticide analysis. Advantages of TLC for pesticide analysis include the ability to analyze multiple samples on a single plate (high throughput); applications to pesticides of different chemical classes in a wide variety of sample matrices because of a great array of layer types, development techniques, detection methods based on physical, chemical, and biological methods, and instrumental techniques for qualitative and quantitative analysis; ease of use and low cost in its manual mode; requirement for only minimal sample cleanup because each plate is used only once; ability to repeat detection and quantification steps under different conditions because the complete chromatograms are stored on the plate; documentation of TLC plates by videoscans or photographs; quantitative analysis by image analysis and densitometry; and spectral analysis of separated zones by multiwavelength scanning of chromatograms.…”