High-performance liquid chromatography and diode-array detection for the identification of peptides containing aromatic amino acids in studies of endorphin-degrading activity in human cerebrospinal fluid

Elevated Cerebrospinal Fluid Substance P-like Immunoreactivity in Patients with Painful Osteoarthritis, but not in Patients with Rhizopatic Pain from a Herniated Lumbar Disc

“…Runs were carried out both on a column for gel filtration chromatography and on one for reversed-phase separation. In the gel filtration step the HPLC system (19) was equipped with a Pharmacia Superdex G-75 column (1, 5 cm x 30 cm) operating at a flow rate of 0.15 ml/min. The column was eluted with 20% acetonitrile containing trifluoroacetic acid (TFA).…”

Application of photodiode array detection and fast atom bombardment mass spectrometry for the identification of the arginine residue in neuropeptides

“…0.SmL fractions were collected at a flow rate of 0.5 mL/min and the samples were subsequently divided into aliquots evaporated in vacuo (Savant-Vac, Hicksville, NY, USA) and stored for further studies. Spectral analysis of the particular peptide derivatives was performed according to the previously described procedures (Nyberg et al, 1986; Silberring et a/. , 1989).…”

Application of high performance liquid chromatography combined with diode‐array detection for analysis of proteins and peptides in human cerebrospinal fluid

“…As suggested by Fell et al, (1983), even derivative spectra can be more easily interpreted as they are more related to zero-order spectra than the odd ones. This, however, may raise the technical problem with detector sensitivity since any derivative requires much higher absorbance signal and, thereby higher peptide concentration than routinely used for neuropeptide degradation studies (Nyberg et al, 1986). The careful testing of peak purity is valuable when radioimmunoassay or radioreceptor assay techniques follow reverse-phase separation.…”

“…The major advantages of the photodiode-array technique are: (I) detection flexibility; (11) post-run adjustment of optimal FSD range; (111) presentation of three-dimensional chromatograms. The usefulness of this method has been recently described for identification of neuropeptides (Nyberg et al, 1986) and for analysis of drugs of toxicological interest (Hill and Langner, 1987). Presently existing methods for CSF analysis utilize isoelectrofocusing (Gallo et aZ., 1985), electrophoresis (Karcher and Lowenthal, 1987) as well as reverse-phase HPLC followed by radioreceptor-assay (e.g., Desiderio et d., 1987;Liu and Desiderio, 1987).…”