High Performance Liquid Chromatographic Determination of Ciprofloxacin Hydrochloride and Ornidazole in Human Plasma

Rote, Ambadas R.

doi:10.4172/2471-2698.1000103

Cited by 7 publications

(4 citation statements)

References 8 publications

(8 reference statements)

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“…Ciprofloxacin is an antibiotic used to treat a number of bacterial infections. This includes bone and joint infections, intra abdominal infections, certain type of infectious diarrhea, respiratory tract infections, skin infections, typhoid fever and urinary tract infections, among others [8][9][10][11]. For some infections it is used in addition to other antibiotics.…”

Section: Fig 1 Chemical Structure Of Metronidazolementioning

confidence: 99%

A Novel Synchronic Estimstion of Metronidazole, Ciprofloxacin and Doxycycline by RP-HPLC in Bulk and Pharmaceutical Formulation

Nadendla

Morla

Patchala

et al. 2021

JPRI

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Aim: To design simple, rapid, new analytical method for estimation of Metronidazole, Ciprofloxacin Doxycycline by using RP-HPLC in bulk and pharmaceutical dosage form. Study Design: Estimation of Metronidazole, Ciprofloxacin Doxycycline by using RP-HPLC in bulk and pharmaceutical dosage form was planned and executed. Place and Duration of Study: Chalapathi Drug Testing Laboratory, Chalapathi Institute Of Pharmaceutical Sciences, Lam, Guntur-522034, Andhra Pradesh, India during the period of November 2019 to February 2020. Methodology: Metronidazole is an antibiotic and antiprotozoal medication. It is used either alone or with other antibiotics to treat pelvic inflammatory disease, endocarditis and bacterial vaginosis. Ciprofloxacin is an antibiotic used to treat a number of bacterial infections. Doxycycline is a tetracycline antibiotic that fights bacteria in the body. The study was carried out on SHIMADZU Prominance-i, LC-2030C system equipped with Shim-pack Gist (250 x 4.6 mm, 5μm) column and mobile phase was optimized by using mixture of methanol and 0.25mM potassium phosphate buffer in the ration of 60:40 v/v at a flow rate of 0.8 ml/min. The wavelength was set as 282nm at ambient temperature by injecting 20µl of solution and the run time was fixed for 10 min. Results: Linearity plot was constructed for concentration range of 5-15µg/ml for Metronidazole,5-15µg/ml for Ciprofloxacin and 1-8µg/ml for Doxycycline standard solutions. It shows best regression coefficient and y/s values. The accuracy of the proposed method was determined by performing recovery studies and was found between 98-102%. The repeatability testing for both sample and standard solutions the %RSD was found as <2.0% which is within the acceptable limits showing that the method is precise as well. The LOD and LOQ were found to be 0.33 and 0.99 µg/ml for Metronidazole, 0.33 and 0.99 µg/ml for Ciprofloxacin, 0.08 and 0.25 µg/ml for Doxycycline respectively. The proposed method was successfully applied for the pharmaceutical dosage form of Metronidazole, Ciprofloxacin Doxycycline and it was as economic, eco-friendly with less retention time around 10.0 min. Conclusion: The proposed method was validated in terms of linearity, range, Accuracy, precision, Specificity, Robustness. Method was successfully applied to the estimation of Metronidazole, Ciprofloxacin Doxycycline in combined marketed pharmaceutical dosage form.

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Section: Fig 1 Chemical Structure Of Metronidazolementioning

confidence: 99%

A Novel Synchronic Estimstion of Metronidazole, Ciprofloxacin and Doxycycline by RP-HPLC in Bulk and Pharmaceutical Formulation

Nadendla

Morla

Patchala

et al. 2021

JPRI

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“…This method can be used for clinical and pharmacokinetic studies. 68 HPLC method for determination of clobetasol in rat plasma and its application to skin penetration: A simple and sensitive HPLC method was developed for quantification of clobetasol (CLB) in rat plasma.…”

Section: Hplc Determination Of Ciprofloxacin Hydrochloride and Ornidazole In Human Plasmamentioning

confidence: 99%

A review of HPLC technique covering its pharmaceutical, environmental, forensic, clinical and other applications

Chawla¹,

Chaudhary²

2019

IJPCA

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HPLC is an analytical technique widely used for identification, separation, detection and quantification of various drugs and its related degradants. Appropriate mobile phase, stationary phase, column, column size, temperature, wavelength and gradient selection is important for the suitable compatibility and stability of drug as well as impurities and degradants. Most of the drugs as well as other compounds can be analyzed by HPLC method because of the several advantages like rapidity, specificity, accuracy, precision and ease of automation in this method. This article has been prepared with an aim to review different aspects of HPLC, such as principle, types, mode of separation, characteristics, instrumentation, important parameters and various applications of HPLC in different fields.

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“…5,6 Literature survey reveals several analytical methods have been developed and validated for estimation of Ciprofloxacin HCl and Ornidazole in bulk, pharmaceutical dosage forms and biological fluids. Ciprofloxacin HCl were analysed by few spectroflourimetric, 7 spectrophotometric 7-9 and HPLC [10][11][12][13][14][15][16][17][18][19][20][21] and HPTLC methods. 22 Ornidazole were estimated by few HPLC 13,14,16,23 and HPTLC 22 methods.…”

Section: Introductionmentioning

confidence: 99%

“…Ciprofloxacin HCl were analysed by few spectroflourimetric, 7 spectrophotometric 7-9 and HPLC [10][11][12][13][14][15][16][17][18][19][20][21] and HPTLC methods. 22 Ornidazole were estimated by few HPLC 13,14,16,23 and HPTLC 22 methods. But no Ultra-Fast Liquid Chromatographic (UFLC) method was reported for estimation of Ciprofloxacin HCl and Ornidazole in combined bulk dosage forms.…”

Section: Introductionmentioning

confidence: 99%

Method Development and Validation of Ciprofloxacin HCL and Ornidazole by UFLC in Combined Dosage Form

Shivabasappa¹,

Diksha²,

Chouhan³

et al. 2019

IJPER

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Background:The combination of Ciprofloxacin and Ornidazole is used for the effective treatment of intra-abdominal infections. Many branded and generic combined dosage formulations of Ciprofloxacin and Ornidazole are available in market. Objective: To develop and validate rapid, sensitive, simple and accurate reverse phase ultra-fast liquid chromatographic method for the determination of Ciprofloxacin and Ornidazole in combined dosage forms. Methods: The separation was carried out by using Phenomenex Luna C 18 (250×4.6 mm, 5 µm) column as stationary phase and acetonitrile: methanol: 0.1% formic acid as a mobile phase in ratio of 35:35:30 % v/v/v. The analysis was performed at flow rate of 0.8 ml/min using PDA detector at 300 nm. The column temperature was maintained at 20°C for better separation and resolution. Results: The retention time for Ciprofloxacin HCl and Ornidazole was found to be 2.1 min and 4.2 min respectively. The developed method was validated with respect to specificity, linearity and range, precision, robustness and accuracy according to ICH guidelines. The method was found to be linear between the concentration ranges of 2-10 µg/ml with correlation coefficient of 0.999. The % relative standard deviation of precision and robustness was found to be less than 2%. The percent accuracy was found to be 98-103% and 99-102% for Ciprofloxacin HCl and Ornidazole respectively. Conclusion: The method is applicable for the routine analysis and quality control of Ciprofloxacin HCl and Ornidazole in combination.

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High Performance Liquid Chromatographic Determination of Ciprofloxacin Hydrochloride and Ornidazole in Human Plasma

Cited by 7 publications

References 8 publications

A Novel Synchronic Estimstion of Metronidazole, Ciprofloxacin and Doxycycline by RP-HPLC in Bulk and Pharmaceutical Formulation

A Novel Synchronic Estimstion of Metronidazole, Ciprofloxacin and Doxycycline by RP-HPLC in Bulk and Pharmaceutical Formulation

A review of HPLC technique covering its pharmaceutical, environmental, forensic, clinical and other applications

Method Development and Validation of Ciprofloxacin HCL and Ornidazole by UFLC in Combined Dosage Form

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