High-performance liquid chromatographic determination of ciprofloxacin in plasma

Mehta, A. C.; Hart‐Davies, S.; Kay, E. A.

doi:10.1111/j.1365-2710.1992.tb01278.x

Cited by 15 publications

(4 citation statements)

References 8 publications

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“…Ciprofloxacin concentrations were measured by means of a high-performance liquid chromatographic assay previously described [14]. Immediately after collection, each blood sample was gently reversed a few times to assure complete mixing with the anticoagulant and was centrifuged for 10 min, at approximately 1,000´g at 3°C to separate the plasma.…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetics and absolute bioavailability of ciprofloxacin administered through a nasogastric tube with continuous enteral feeding to critically ill patients

Mimoz

Binter

Jacolot

et al. 1998

Intensive Care Medicine

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Section: Methodsmentioning

confidence: 99%

Pharmacokinetics and absolute bioavailability of ciprofloxacin administered through a nasogastric tube with continuous enteral feeding to critically ill patients

Mimoz

Binter

Jacolot

et al. 1998

Intensive Care Medicine

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“…It is also a common Tibetan medicine and has been used to treat stomach pain and blood disorders. In Mongolian medicine, the roots of S. lappa have been used to treat lung abscesses and phlegm [ 16 ]. Some eudesmane-, guaiane- and germacane-type sesquiterpenes have been isolated from S. lappa .…”

Section: Introductionmentioning

confidence: 99%

“…Some eudesmane-, guaiane- and germacane-type sesquiterpenes have been isolated from S. lappa . These compounds showed potential effects on chronic superficial gastritis, ulcer, cancer, bacterial, fungal and viral diseases [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Structure-Based Molecular Networking for the Discovery of Anti-HBV Compounds from Saussurea lappa (Decne.) C.B Clarke

Yan

Wang

et al. 2022

Molecules

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It is a crucial to find target compounds in natural product research. This study presents a concept of structure-guided isolation to find candidate active molecules from herbs. We establish a process of anti-viral sesquiterpene networking. An analysis of the networking suggested that new anti-HBV sesquiterpene may be attributable to eudesmane-, guaiane-, cadinane-, germacane- and bisabolane-type sesquiterpenes. In order to evaluate the efficiency of the structure-based molecular networking, ethanol extract of Saussurea lappa (Decne.) C.B Clarke was investigated, which led to the isolation of two guaiane-type (1 and 14), ten eudesmane-type (2–5 and 8–13), two chain (6 and 7) and one germacrane-type (15) sesquiterpenes, including seven new ones, lappaterpenes A–G (1–7), which are reported on herein. The absolute configurations of the new compounds were established by coupling constants, calculated ECD and ROESY correlations, as well as comparisons of optical rotation values with those of known compounds. The absolute configuration of compound 2 was further confirmed by X-ray diffraction. Compounds 1–15 were evaluated for their potency against hepatitis B virus. Compounds 4, 6, 7 and 9 showed effect on HBsAg with inhibition ratios of more than 40% at 30 μM concentrations. Compounds 14 and 15 inhibited HBsAg secretion with the values of IC50 0.73 ± 0.18 and 1.43 ± 0.54 μM, respectively. Structure-based molecular networking inspired the discovery of target compounds.

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“…This enzyme is responsible for the negative super coiling of the bacterial DNA and consequently for its topological configuration, governing functions such as RNA transcription, protein synthesis, DNA replication and repair functions [2]. The literature survey revealed that variety of analytical methods reported for estimation of ciprofloxacin in human plasma and other biological fluids [3][4][5][6][7][8], spectrophotometry [9,10], HPLC [11][12][13], HPTLC [14], and spectrophotometric methods [15][16][17] have been reported for estimation of ornidazole alone as well as in combinations. RP-HPLC method was reported for estimation of Ciprofloxacin Hydrochloride and Ornidazole in Combined Pharmaceutical Dosage Form [18].…”

Section: Introductionmentioning

confidence: 99%

High Performance Liquid Chromatographic Determination of Ciprofloxacin Hydrochloride and Ornidazole in Human Plasma

Rote¹

2015

Pharm Anal Chem

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A simple, rapid, sensitive selective chromatographic method has been developed for simultaneous determination of ciprofloxacin hydrochloride and ornidazole in human plasma by using internal standard. The method depends on reverse phase high performance liquid chromatography. The plasma sample was extracted using methanol: formic acid (5.5:0.5, v/v). A concentration range from 100-400 ng/ml for both drugs was used for calibration curve. The percent recoveries of ciprofloxacin and ornidazole were found to be 71.49-75.68 and 73.78-83.1 respectively. The mobile used consist of acetonitrile: methanol: water: triethylamine (40:20:40: 1% v/v/v) and flow rate 1 ml/min in isocratic mode. The separation was carried out by UV detector at wavelength 300 nm. The stability of ciprofloxacin hydrochloride and ornidazole in plasma were confirmed during three freeze-thaw cycles (-20°C), on bench during 12 h, and post preparative stability study. The proposed method was validated statistically and by performing recoveries study for determination of ciprofloxacin hydrochloride and ornidazole in human plasma.

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High-performance liquid chromatographic determination of ciprofloxacin in plasma

Cited by 15 publications

References 8 publications

Pharmacokinetics and absolute bioavailability of ciprofloxacin administered through a nasogastric tube with continuous enteral feeding to critically ill patients

Pharmacokinetics and absolute bioavailability of ciprofloxacin administered through a nasogastric tube with continuous enteral feeding to critically ill patients

Structure-Based Molecular Networking for the Discovery of Anti-HBV Compounds from Saussurea lappa (Decne.) C.B Clarke

High Performance Liquid Chromatographic Determination of Ciprofloxacin Hydrochloride and Ornidazole in Human Plasma

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