High-performance liquid chromatographic assay for the determination of Aloe Emodin in mouse plasma

Zaffaroni, Marco; Mucignat‐Caretta, Carla; Pecere, Teresa; Zagotto, Giuseppe; Frapolli, Roberta; D’Incalci, Maurizio; Zucchetti, Massimo

doi:10.1016/j.jchromb.2003.08.012

Cited by 25 publications

(16 citation statements)

References 4 publications

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“…These ranges were found to be adequate for the concentrations observed from the analysis of collected plasma samples. It showed considerable linearity and had similar precision and accuracy to those reported in a study by Zaffaroni et al (2003).…”

Section: Calibration Curvessupporting

confidence: 79%

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Simultaneous determination and pharmacokinetic studies of aloe emodin and chrysophanol in rats after oral administration of Da‐Cheng‐Qi decoction by high‐performance liquid chromatography

Tang

Wan

et al. 2007

Biomedical Chromatography

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A validated high-performance liquid chromatography (HPLC) method was developed for simultaneous determination and pharmacokinetic study of aloe emodin and chrysophanol in rats. It was performed on a reverse-phase C(18) column and a mobile phase made up of methanol and 0.2% acetic acid (83:17, v/v). The ultraviolet detection was 254 nm. 1,8-dihydroxyanthraquinone was used as the internal standard. The assay was linear over the range 28-2800 ng/mL (r(2) = 0.9993) for aloe emodin and 25.6-2560 ng/mL (r(2) = 0.9991) for chrysophanol. The average percentage recoveries of three spiked plasmas were 98.8-104.8% and 97.7-103.2% for aloe emodin and chrysophanol, respectively. Their RSD of intra-day and inter-day precision at concentrations of 56, 280 and 1400 ng/mL for aloe emodin and 51.6, 258 and 1290 ng/mL for chrysophanol were less than 3.5%. This method was applied for the first time to simultaneously determinate aloe emodin and chrysophanol in rats following oral administration of traditional Chinese medicine of Da-Cheng-Qi decoction. The pharmacokinetic parameters showed that chrysophanol was better absorbed with higher concentrations in plasma than aloe emodin did. They both eliminated slowly in male rats. The assay is suitable for identifying the plasma and tissue levels of aloe emodin and chrysophanol in preclinical investigations.

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Section: Calibration Curvessupporting

confidence: 79%

“…DCQD has many potential effective components, including aloe emodin and chrysophanol from Dahuang. The pharmacokinetics of aloe emodin after intraperitoneal administration has been reported (Zaffaroni et al, 2003). In humans, no aloe emodin was detected in the plasma after the administration of Agiolax and Sennatin (Krumbiegel and Schulz, 1993).…”

mentioning

confidence: 99%

Simultaneous determination and pharmacokinetic studies of aloe emodin and chrysophanol in rats after oral administration of Da‐Cheng‐Qi decoction by high‐performance liquid chromatography

Tang

Wan

et al. 2007

Biomedical Chromatography

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“…Figure 2 shows the mean concentrationtime profiles of anthraquinones in five rats after a single However, there were some limitations in those reported methods. Some methods were reported for the determination of rhein (Lee et al, 2003;Zhang et al, 2005), emodin and chrysophanol (Pang et al, 1993) and aloeemodin (Zaffaroni et al, 2003) in plasma, respectively. However, they are not suitable for pharmacokinetic study of multiple components from traditional Chinese decoction containing Rhei Rhizoma, since only one or two kinds of anthraquinones can be determined at the same time.…”

Section: Application To Pharmacokinetic Studymentioning

confidence: 99%

Simultaneous quantification of five anthraquinones in rat plasma by high‐performance liquid chromatography with fluorescence detection

Yan

2007

Biomedical Chromatography

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A sensitive high-performance liquid chromatographic method with fluorescence detection (excitation 435 and emission 515 nm) was established and validated for quantification of five anthraquinones (aloe-emodin, rhein, emodin, chrysophanol and physcion) in rat plasma. Following a single-step liquid-liquid extraction, the analytes and internal standard (1,8-dihydroxyanthraquinone) were separated on a reversed-phase C(18) column with water-phosphoric acid-methanol as mobile phase at a flow rate of 1 mL/min. The linear ranges of the calibration curves were 6.5-1300 ng/mL for aloe-emodin, 20-4000 ng/mL for rhein, 40-8000 ng/mL for emodin, 15-3000 ng/mL for chrysophanol and 13-2600 ng/mL for physcion. The lower limit of quantification was 6.5 ng/mL for aloe-emodin, 20 ng/mL for rhein, 40 ng/mL for emodin, 15 ng/mL for chrysophanol and 13 ng/mL for physcion. The mean accuracy was 94.3-105.1% for aloe-emodin, 90.3-108.8% for rhein, 92.6-106.7% for emodin, 95.8-103.8% for chrysophanol and 98.7-101.2% for physcion. The within-batch and between-batch precisions were < or = 5.5% and < or = 13.4%, respectively. This method is suitable for determining the five anthraquinones in plasma simultaneously and thus investigating the pharmacokinetics of anthraquinones from Xiexin decoction in rats.

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“…The limits of detection (LOD) was determined on the basis of the signal-to-noise ratio obtained by injecting methanol as a sample into the HPLC system [14,15]. The limits of quantification (LOQ) was defined as the lowest drug concentration that can be determined reproducibly (coefficient of variation <20%) and accurately (% error <20%) [16,17]. Within and between day precision as well as accuracy was evaluated by running five replicates of QCs at three different C1APT concentrations (i.e., 25, 250, and 1000 ng mL −1 ).…”

Section: Assay Validationmentioning

confidence: 99%

A simple HPLC method for determining 2-(3-chlorophenyloamino)-5-(2,4-dihydroxyphenyl)-1,3,4-thiadiazole in brain and plasma of animals: Application to a pharmacokinetic study

Raszewski

Juszczak²,

Lemieszek³

et al. 2014

Acta Chromatographica

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Summary. The development, optimization, and validation of a new high-performance liquid chromatography ultraviolet (HPLC-UV) method is presented for determining 2-(3-chlorophenyloamino)-5-(2,4-dihydroxy-phenyl)-1,3,4-thiadiazole (ClABT) in biological samples of rat plasma and brain tissue. ClABT was extracted directly from a plasma supernatant fraction following protein precipitation with acetonitrile and high speed centrifugation. Reverse phase HPLC separation was performed using an ODS-2 Hypersil column with the mobile phase consisting of 0.05 M triethylammonium phosphate buffer solution in acetonitrile and methanol (120:280:600, v/v/v), at room temperature, 1.2 mL min −1 flow rate, and UV-diode-array detection (DAD), at 335 nm. A linear response was obtained between 12.5 and 2000 ng mL −1 at analytically acceptable levels of precision (intra/inter-day) and accuracy. Mean recoveries ranged from 92.7% to 107.9%. It was concluded that the method was specific and precise and thus suitable for quantitative analysis in clinical pharmacokinetic studies of ClABT.

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High-performance liquid chromatographic assay for the determination of Aloe Emodin in mouse plasma

Cited by 25 publications

References 4 publications

Simultaneous determination and pharmacokinetic studies of aloe emodin and chrysophanol in rats after oral administration of Da‐Cheng‐Qi decoction by high‐performance liquid chromatography

Simultaneous determination and pharmacokinetic studies of aloe emodin and chrysophanol in rats after oral administration of Da‐Cheng‐Qi decoction by high‐performance liquid chromatography

Simultaneous quantification of five anthraquinones in rat plasma by high‐performance liquid chromatography with fluorescence detection

A simple HPLC method for determining 2-(3-chlorophenyloamino)-5-(2,4-dihydroxyphenyl)-1,3,4-thiadiazole in brain and plasma of animals: Application to a pharmacokinetic study

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