High Levels of Antibodies Against Merkel Cell Polyomavirus Identify a Subset of Patients With Merkel Cell Carcinoma With Better Clinical Outcome

Touzé, Antoine; Bidre, Emmanuelle Le; Laude, Hélène; Fleury, Maxime; Cazal, R; Arnold, Françoise; Carlotti, A.; Maubec, Ève; Aubin, F.; Avril, Marie-Françoise; Rozenberg, Flore; Tognon, Mauro; Maruani, Annabel; Guyétant, Serge; Lorette, G.; Coursaget, Pierre

doi:10.1200/jco.2010.31.1704

Cited by 148 publications

(133 citation statements)

References 37 publications

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“…The mean viral load has been reported to be 1-5 copies per cell, in agreement with integration of the viral genome into chromosomal DNA (1,90,93,94). Anti-VP1 antibodies have been detected in 88-100% of MCC cases (7,8,14,25). In addition, the capsid antibody titer in many of these MCC cases is much higher than observed in control populations.…”

Section: Mcpyv Viral Markers In MCC Patientssupporting

confidence: 48%

“…In addition, the capsid antibody titer in many of these MCC cases is much higher than observed in control populations. The anti-VP1 geometric mean titer in MCC patients is 14-59 times higher than in controls (7,8). One explanation is that loss of immune surveillance in some patients enhances active replication of the virus and, as a consequence, the recombination of virus genome fragments with the host genome.…”

Section: Mcpyv Viral Markers In MCC Patientsmentioning

confidence: 99%

“…MCC is a relatively rare but aggressive form of cutaneous cancer, and the incidence of this cancer increases with age, immunodeficiency and sun exposure (4)(5)(6). MCC tumor cells do not seem to produce viral particles (7,8) and the specific cells in which MCPyV virions are produced have not been identified to date. It has also been shown that UV irradiation induces an increase in MCPyV small T antigen (sTag) transcripts that may explain the association between sun exposure, MCPyV infection, and the development of MCC (9).…”

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confidence: 99%

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Human Merkel cell polyomavirus: virological background and clinical implications

Coursaget

Samimi

Nicol

et al. 2013

APMIS

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The Merkel cell polyomavirus (MCPyV), identified in humans in 2008, is associated with a relatively rare but aggressive neuroendocrine skin cancer, the Merkel cell carcinoma (MCC). MCC incidence is increasing due to the advancing age of the population, the increase in damaging sun exposure and in the number of immunocompromised individuals. MCPyV must be considered as the etiological agent of MCC and thus is the first example of a human oncogenic polyomavirus. MCPyV infection is common, and seroprevalence studies indicate that widespread exposure begins early in life. The majority of adults have anti-MCPyV antibodies and there is a growing body of evidence that healthy human skin harbors resident or transient MCPyV suggesting that MCPyV infection persists throughout life. However, the mode of transmission, the host cells, and the latency characteristics of this virus remain to be elucidated. In addition, it is still not clear whether MCPyV is associated with diseases or lesions other than Merkel cell carcinoma. The etiologic role of MCPyV in MCC opens up opportunities to improve the understanding of this cancer and to potentially improve its treatment.

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Section: Mcpyv Viral Markers In MCC Patientssupporting

confidence: 48%

Section: Mcpyv Viral Markers In MCC Patientsmentioning

confidence: 99%

mentioning

confidence: 99%

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Human Merkel cell polyomavirus: virological background and clinical implications

Coursaget

Samimi

Nicol

et al. 2013

APMIS

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“…Moreover, patients with MCC with high MCPyV antibody titers may present better progression-free survival (34).…”

Section: Discussionmentioning

confidence: 99%

Merkel Cell Carcinomas Arising in Autoimmune Disease Affected Patients Treated with Biologic Drugs, Including Anti-TNF

Rotondo

Bononi

Puozzo

et al. 2017

Clinical Cancer Research

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Purpose: The purpose of this investigation was to characterize Merkel cell carcinomas (MCC) arisen in patients affected by autoimmune diseases and treated with biologic drugs.Experimental Design: Serum samples from patients with MCC were analyzed for the presence and titer of antibodies against antigens of the oncogenic Merkel cell polyomavirus (MCPyV). IgG antibodies against the viral oncoproteins large T (LT) and small T (ST) antigens and the viral capsid protein-1 were analyzed by indirect ELISA. Viral antigens were recombinant LT/ST and viruslike particles (VLP), respectively. MCPyV DNA sequences were studied using PCR methods in MCC tissues and in peripheral blood mononuclear cells (PBMC). Immunohistochemical (IHC) analyses were carried out in MCC tissues to reveal MCPyV LT oncoprotein.Results: MCPyV DNA sequences identified in MCC tissues showed 100% homology with the European MKL-1 strain.PBMCs from patients tested MCPyV-negative. Viral DNA loads in the three MCC tissues were in the 0.1 to 30 copy/cell range. IgG antibodies against LT/ST were detected in patients 1 and 3, whereas patient 2 did not react to the MCPyV LT/ST antigen. Sera from the three patients with MCC contained IgG antibodies against MCPyV VP1. MCC tissues tested MCPyV LT-antigenpositive in IHC assays, with strong LT expression with diffuse nuclear localization. Normal tissues tested MCPyV LT-negative when employed as control.Conclusions: We investigated three new MCCs in patients affected by rheumatologic diseases treated with biologic drugs, including TNF. A possible cause-effect relationship between pharmacologic immunosuppressive treatment and MCC onset is suggested. Indeed, MCC is associated with MCPyV LT oncoprotein activity.

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“…Other variables, exemplified by the growing literature regarding associations between the Merkel cell polyoma virus and outcomes in MCC, should be included. 11 Whenever possible, tissue banking of fresh tumor specimens is to be strongly supported. With regard to treatment, we continue to advocate offering SLNB to all patients with clinically localized MCC, as the implications of nodal basin microstaging are as yet not completely understood in this nodotrophic tumor.…”

mentioning

confidence: 99%

Is a “Merkel” Just Like a Melanoma? The Pathologic Analysis of Merkel Cell Carcinoma Specimens

Fields

Coit

2012

Ann Surg Oncol

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High Levels of Antibodies Against Merkel Cell Polyomavirus Identify a Subset of Patients With Merkel Cell Carcinoma With Better Clinical Outcome

Cited by 148 publications

References 37 publications

Human Merkel cell polyomavirus: virological background and clinical implications

Human Merkel cell polyomavirus: virological background and clinical implications

Merkel Cell Carcinomas Arising in Autoimmune Disease Affected Patients Treated with Biologic Drugs, Including Anti-TNF

Is a “Merkel” Just Like a Melanoma? The Pathologic Analysis of Merkel Cell Carcinoma Specimens

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