High level of laccases production by Trametes trogii culture on olive mill wastewater‐based media, application in textile dye decolorization

Abstract

BACKGROUND: Laccases are receiving increasing attention as potential industrial enzymes in various applications. Therefore, it is important to find inexpensive and optimized media for large-scale commercial production. The present work aims to valorize olive mill wastewater (OMW) by its use as base media for laccases production by Trametes trogii and use of the laccases produced for decolorizing textile dyes.

Trametes trogii: A Biologic Powerful Tool for Dyes Decolorization and Detoxification

“…The ability of Trametes trogii to decolorize industrial dyes was confirmed by several studies [5,[9][10][11][12]. Thus, strain T. trogii proved to be an efficient tool for the dye degradations such as Remazol Brilliant Blue R (RBBR) [9], blue solophenyl, blue tubantin GLL 300, black tubantin VSF 600, yellow solophenyl and red BLL [11], Neolane blue, Neolane pink, Neolane yellow, Maxilon blue, Basacryl yellow and Bezaktiv S-BF turquoise [10].…”

“…Copper ion was necessary for laccase activity [17]. Previous results obtained, using copper T. trogii laccase inducer, have clearly demonstrated that laccase is seemingly the key enzyme responsible for the efficient decolorization [9][10][11]16]. …”

“…It has found that neither manganese peroxidase nor lignin peroxidase were detected during T. trogii growth. Laccase was the unique lignin modifying enzyme produced by this fungus, under specific conditions and in the presence of copper and ethanol as inducers [10,11]. Laccase belongs to the family of blue multi-copper oxidases which contain four copper atoms in the catalytic centre per molecule.…”

“…This strain was selected for this study due to its powerful potential to decolorize dyes [9][10][11] and effluents [16] from textile industries and to produce a high amount of laccase under optimized conditions [10]. The laccase production and characterization were carried out as previously described [10].…”

“…Thus, strain T. trogii proved to be an efficient tool for the dye degradations such as Remazol Brilliant Blue R (RBBR) [9], blue solophenyl, blue tubantin GLL 300, black tubantin VSF 600, yellow solophenyl and red BLL [11], Neolane blue, Neolane pink, Neolane yellow, Maxilon blue, Basacryl yellow and Bezaktiv S-BF turquoise [10]. Laccase from T. trogii was reported to have one of the highest redox potential among laccases (E°0.79 at pH 5.0) [13], which make this laccase particularly interesting since high redox potentials correlate with high laccase activity [13,14].…”