High hydrostatic pressure increases amino acid requirements in the piezo-hyperthermophilic archaeon Thermococcus barophilus

Cario, Anaïs; Lormières, Florence; Xiao, Xiang; Oger, Philippe

doi:10.1016/j.resmic.2015.07.004

Cited by 19 publications

(18 citation statements)

References 27 publications

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“…Only a handful of piezo-thermophiles have been studied, mainly belonging to the Archaea domain ( e.g. Thermococcus barophilus MP) 3 4 . The most HHP-impacted cell component is the cytoplasmic membrane, which tends to become rigid due to the compaction of its lipid constituents in piezosensitive Bacteria 1 .…”

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confidence: 99%

High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii

Michoud

Jebbar

2016

Sci Rep

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Pyrococcus yayanosii CH1, as the first and only obligate piezophilic hyperthermophilic microorganism discovered to date, extends the physical and chemical limits of life on Earth. It was isolated from the Ashadze hydrothermal vent at 4,100 m depth. Multi-omics analyses were performed to study the mechanisms used by the cell to cope with high hydrostatic pressure variations. In silico analyses showed that the P. yayanosii genome is highly adapted to its harsh environment, with a loss of aromatic amino acid biosynthesis pathways and the high constitutive expression of the energy metabolism compared with other non-obligate piezophilic Pyrococcus species. Differential proteomics and transcriptomics analyses identified key hydrostatic pressure-responsive genes involved in translation, chemotaxis, energy metabolism (hydrogenases and formate metabolism) and Clustered Regularly Interspaced Short Palindromic Repeats sequences associated with Cellular apoptosis susceptibility proteins.

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confidence: 99%

High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii

Michoud

Jebbar

2016

Sci Rep

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“…Metabolic adjustment at the global scale has been shown to be a response to pressure stress in T. barophilus (Vannier et al, 2015). Moreover, HHP increases amino acid requirements in T. barophilus MP (Cario et al, 2015). Several amino acid biosynthesis pathways are missing in the P. yayanosii genome (Michoud and Jebbar, 2016).…”

Section: Discussionmentioning

confidence: 99%

An Integrative Genomic Island Affects the Adaptations of the Piezophilic Hyperthermophilic Archaeon Pyrococcus yayanosii to High Temperature and High Hydrostatic Pressure

Xiao

et al. 2016

Front. Microbiol.

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Deep-sea hydrothermal vent environments are characterized by high hydrostatic pressure and sharp temperature and chemical gradients. Horizontal gene transfer is thought to play an important role in the microbial adaptation to such an extreme environment. In this study, a 21.4-kb DNA fragment was identified as a genomic island, designated PYG1, in the genomic sequence of the piezophilic hyperthermophile Pyrococcus yayanosii. According to the sequence alignment and functional annotation, the genes in PYG1 could tentatively be divided into five modules, with functions related to mobility, DNA repair, metabolic processes and the toxin-antitoxin system. Integrase can mediate the site-specific integration and excision of PYG1 in the chromosome of P. yayanosii A1. Gene replacement of PYG1 with a SimR cassette was successful. The growth of the mutant strain ΔPYG1 was compared with its parent strain P. yayanosii A2 under various stress conditions, including different pH, salinity, temperature, and hydrostatic pressure. The ΔPYG1 mutant strain showed reduced growth when grown at 100°C, while the biomass of ΔPYG1 increased significantly when cultured at 80 MPa. Differential expression of the genes in module III of PYG1 was observed under different temperature and pressure conditions. This study demonstrates the first example of an archaeal integrative genomic island that could affect the adaptation of the hyperthermophilic piezophile P. yayanosii to high temperature and high hydrostatic pressure.

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“…This study reported an unexpected taxonomic-based dichotomic response to pressure with piezosensitve ascomycetes and piezotolerant basidiomycetes, and distinct morphological switches triggered by pressure for certain strains [5]. Cario et al, explored the amino acid requirements of a hyperthermophilic piezophilic archaeon Thermococcus barophilus strain MP and they showed a major impact of HHP on amino acid metabolism, with increases from three amino acids required at atmospheric pressure to seventen at 40 MPa [6]. Only alanine, glutamine, and proline, were not essential for growth at 40 MPa, in contrast, glutamate, cysteine and tyrosine, were essential for growth at atmospheric pressure [6].…”

Section: Accepted Manuscriptmentioning

confidence: 97%

“…Cario et al, explored the amino acid requirements of a hyperthermophilic piezophilic archaeon Thermococcus barophilus strain MP and they showed a major impact of HHP on amino acid metabolism, with increases from three amino acids required at atmospheric pressure to seventen at 40 MPa [6]. Only alanine, glutamine, and proline, were not essential for growth at 40 MPa, in contrast, glutamate, cysteine and tyrosine, were essential for growth at atmospheric pressure [6]. Vannier et al, showed that the HHP response in the T. barophilus is characterized by a global change in the expression of genes involved M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT in some metabolic pathways, rather than expression of a stress response [7].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Deep sea, the last great unexplored earth frontier harboring the largest unknown and untapped remote microbial diversity on our planet

Jebbar

2015

Research in Microbiology

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High hydrostatic pressure increases amino acid requirements in the piezo-hyperthermophilic archaeon Thermococcus barophilus

Cited by 19 publications

References 27 publications

High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii

High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii

An Integrative Genomic Island Affects the Adaptations of the Piezophilic Hyperthermophilic Archaeon Pyrococcus yayanosii to High Temperature and High Hydrostatic Pressure

Deep sea, the last great unexplored earth frontier harboring the largest unknown and untapped remote microbial diversity on our planet

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