High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii

Abstract

Pyrococcus yayanosii CH1, as the first and only obligate piezophilic hyperthermophilic microorganism discovered to date, extends the physical and chemical limits of life on Earth. It was isolated from the Ashadze hydrothermal vent at 4,100 m depth. Multi-omics analyses were performed to study the mechanisms used by the cell to cope with high hydrostatic pressure variations. In silico analyses showed that the P. yayanosii genome is highly adapted to its harsh environment, with a loss of aromatic amino acid biosynthesis pathways and the high constitutive expression of the energy metabolism compared with other non-obligate piezophilic Pyrococcus species. Differential proteomics and transcriptomics analyses identified key hydrostatic pressure-responsive genes involved in translation, chemotaxis, energy metabolism (hydrogenases and formate metabolism) and Clustered Regularly Interspaced Short Palindromic Repeats sequences associated with Cellular apoptosis susceptibility proteins.

An Integrative Genomic Island Affects the Adaptations of the Piezophilic Hyperthermophilic Archaeon Pyrococcus yayanosii to High Temperature and High Hydrostatic Pressure

“…The transcription levels of the genes in module III (PYCH_15220 to PYCH_15270) were up-regulated under low pressure (0.1 MPa, 95°C) and high pressure (70 MPa, 95°C) conditions compared with the optimal conditions (52 MPa, 95°C). These results were consistent with the transcriptomic study of P. yayanosii , in which it was shown that the transcription levels of PYCH_15210, PYCH_15270, and PYCH_15290 in PYG1 under 20 and 80 MPa conditions were higher than those observed under 52 MPa conditions (Michoud and Jebbar, 2016). Under high temperature stress, the expression of PYCH_15260 and PYCH_15270 showed obvious up-regulation at 100°C.…”

“…Moreover, HHP increases amino acid requirements in T. barophilus MP (Cario et al, 2015). Several amino acid biosynthesis pathways are missing in the P. yayanosii genome (Michoud and Jebbar, 2016). PYCH_15260 is annotated as a class II glutamine amidotransferase.…”

The effect of O2 and pressure on thiosulfate oxidation by Thiomicrospira thermophila

“…Moreover, the effect of high hydrostatic pressure on growth and metabolism is less frequently investigated due to the difficulties involved in the experimental design (Takai et al, 2008). For example, previous studies of piezophilic micro-organisms have suggested that energy production and central metabolism-related genes appear to be overexpressed under high hydrostatic pressures (Amrani et al, 2014;Cario, Jebbar, Thiel, Kervarec, & Oger, 2016;Michoud & Jebbar, 2016;Pradel et al, 2013;Vannier, Michoud, Oger, Marteinsson, & Jebbar, 2015). A recent study involving high-pressure culturing of a piezophilic and chemolithoautotrophic epsilonproteobacterium (Nautilia sp.…”

Thermostable iron oxide nanoparticle synthesis within recombinant ferritins from the hyperthermophile Pyrococcus yayanosii CH1

“…In this study, we report a novel ferritin (PcFn) from the hyperthermophile Pyrococcus yayanosii CH1, which was isolated from a vent eld at a depth of 4100 m and characterized as the rst obligate piezophilic hyperthermophilic microorganism (optima of 52 MPa and 98 C). [33][34][35] According to these extremely harsh living conditions, we hypothesized that the ferritin and magnetoferritin from P. yayanosii CH1 might be resistant to high temperatures. To test this hypothesis, we cloned and overexpressed the PcFn gene from hyperthermophile P. yayanosii CH1 in Escherichia coli (E. coli), and successfully used the puried PcFn protein for biomimetic synthesis of magnetoferritin (M-PcFn 5000 ), with a loading factor of 5000 Fe/cage.…”