High frequency transformation and regeneration of transgenic plants in the model legume<i>Lotus japonicus</i>

Stiller, Jiri; Martirani, Luca; Tuppale, Sunil; Chian, Ru‐Ju; Chiurazzi, Maurizio; Gresshoff, Peter M.

doi:10.1093/jxb/48.7.1357

Cited by 150 publications

(109 citation statements)

References 40 publications

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“…LjMYB14 was then transferred to a plant expression vector, pUB-GW-Hyg (Maekawa et al, 2008), via pDONR207 (Invitrogen) using the primers attBMyb14fwd (59-GGGACAAGTTTGTA-CAAAAAAGCAGGCTTAATGGTAAGAGCTCCTTGT-39) and attBMyb14rev (59-GGGGACCACTTTGTACAAGAAAGCTGGGTATCAGAACTCGGGCAGTTC-39) to generate the plant expression vector pUBI-MYB14. pUBI-MYB14 was transformed into Agrobacterium tumefaciens GV3101 for transformation of Lotus (Stiller et al, 1997), ecotype MG20.…”

Section: Cloning Of Ljmyb14mentioning

confidence: 99%

Transcription Factors of Lotus: Regulation of Isoflavonoid Biosynthesis Requires Coordinated Changes in Transcription Factor Activity

et al. 2012

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Isoflavonoids are a class of phenylpropanoids made by legumes, and consumption of dietary isoflavonoids confers benefits to human health. Our aim is to understand the regulation of isoflavonoid biosynthesis. Many studies have shown the importance of transcription factors in regulating the transcription of one or more genes encoding enzymes in phenylpropanoid metabolism. In this study, we coupled bioinformatics and coexpression analysis to identify candidate genes encoding transcription factors involved in regulating isoflavonoid biosynthesis in Lotus (Lotus japonicus). Genes encoding proteins belonging to 39 of the main transcription factor families were examined by microarray analysis of RNA from leaf tissue that had been elicited with glutathione. Phylogenetic analyses of each transcription factor family were used to identify subgroups of proteins that were specific to L. japonicus or closely related to known regulators of the phenylpropanoid pathway in other species. R2R3MYB subgroup 2 genes showed increased expression after treatment with glutathione. One member of this subgroup, LjMYB14, was constitutively overexpressed in L. japonicus and induced the expression of at least 12 genes that encoded enzymes in the general phenylpropanoid and isoflavonoid pathways. A distinct set of six R2R3MYB subgroup 2-like genes was identified. We suggest that these subgroup 2 sister group proteins and those belonging to the main subgroup 2 have roles in inducing isoflavonoid biosynthesis. The induction of isoflavonoid production in L. japonicus also involves the coordinated down-regulation of competing biosynthetic pathways by changing the expression of other transcription factors.

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Section: Cloning Of Ljmyb14mentioning

confidence: 99%

Transcription Factors of Lotus: Regulation of Isoflavonoid Biosynthesis Requires Coordinated Changes in Transcription Factor Activity

et al. 2012

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“…Hypocotyl explants of L. japonicus were transformed with A. tumefaciens strain AGL1 bearing pPZP112nopL or pPZP112 and were regenerated as described (Handberg and Stougaard, 1992;Stiller et al, 1997). Plantlets regenerated from independent transformation events were propagated under greenhouse conditions and were allowed to self-fertilize, yielding seeds of lines carrying pPZP112nopL and pPZP112, respectively.…”

Section: Transformation Of Plantsmentioning

confidence: 99%

NopL, an Effector Protein ofRhizobiumsp. NGR234, Thwarts Activation of Plant Defense Reactions

et al. 2004

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Bacterial effector proteins delivered into eukaryotic cells via bacterial type III secretion systems are important virulence factors in plant-pathogen interactions. Type III secretion systems have been found in Rhizobium species that form symbiotic, nitrogen-fixing associations with legumes. One such bacterium, Rhizobium sp. NGR234, secretes a number of type III effectors, including nodulation outer protein L (NopL, formerly y4xL). Here, we show that expression of nopL in tobacco (Nicotiana tabacum) prevents full induction of pathogenesis-related (PR) defense proteins. Transgenic tobacco plants that express nopL and were infected with potato virus Y (necrotic strain 605) exhibited only very low levels of chitinase (class I) and ␤-1,3-glucanase (classes I and III) proteins. Northern-blot analysis indicated that expression of nopL in plant cells suppresses transcription of PR genes. Treatment with ethylene counteracted the effect of NopL on chitinase (class I). Transgenic Lotus japonicus plants that expressed nopL exhibited delayed development and low chitinase levels. In vitro experiments showed that NopL is a substrate for plant protein kinases. Together, these data suggest that NopL, when delivered into the plant cell, modulates the activity of signal transduction pathways that culminate in activation of PR proteins.Plants have evolved many defenses against invading pathogens. Among them are preformed antimicrobial compounds, as well as inducible defense proteins, the so-called pathogenesis-related (PR) proteins (van Loon, 1997). It has been shown, for example, that pathogen-induced chitinases (EC 3.2.1.14) and ␤-1,3-glucanases (EC 3.2.1.39) synergistically lyse fungal cell walls (Mauch et al., 1988). Specific recognition of pathogens often induces a hypersensitive response, which is characterized by an oxidative burst and localized death of the host cells. In most cases, the induction of a hypersensitive response arrests invasion by the pathogen (Dangl and Jones, 2001).Virulence in gram-negative bacteria often depends on proteins injected into eukaryotic cells. Some bacteria elaborate a specialized protein secretion apparatus, the type III secretion system (TTSS). The TTSS exports a set of proteins from the bacteria, some of which are delivered directly into the eukaryotic cells-a process called translocation (Cornelis and van Gijsegem, 2000;Plano et al., 2001). Most translocated type III effectors act on the cytoskeleton or interfere with intracellular signaling cascades of the host cell. Yersinia pestis, for example, injects at least six type III effectors into host cells, where they thwart the signaling machinery of the immune system (Cornelis, 2002). Plant pathogens such as Pseudomonas syringae and Xanthomonas campestris also use TTSSs (Kjemtrup et al., 2000; Lahaye and Bonas, 2001). Mounting evidence suggests that type III effectors translocated into plant cells (Casper-Lindley et al., 2002;Szurek et al., 2002) act as virulence factors in susceptible hosts. One likely function of type III effectors is ...

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“…Therefore, A. rhizogenes can be used in genetic manipulation of higher plants for improving plant resistance, quality, and possible utilization. Many reports about hairy root transformation of forage legume have been published, including alfalfa [4], common Sainfoin ( Onobrychis viciaefolia) [5] and model legume Lotus japonicus [6]. However, A. rhizogenes mediated transformation of Alhagi pseudoalhagi has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Regeneration of plants from callus cultures of roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi

Wang

Luo

et al. 2001

Cell Res

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The legume forage Alhagi pseudoalhagi was transformed by the Agrobacterium rhizogenes strain A4 using cotyledon and hypocotyl segments as infection materials. Plant regeneration was achieved from sterile calluses derived from hairy roots, which occurred at or near the infection sites. The hairy root regenerants were characterized by normal leaf morphology and stem growth but a shallow and more extensive root system than normal plants. Opine synthesis, PCR and Southern blot confirmed that T-DNA had been intergrated into the A. pseudoalhagi genome. Acetosyringone (AS) was found to be vital for successful transformation of A. pseudoalhagi.

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High frequency transformation and regeneration of transgenic plants in the model legumeLotus japonicus

Cited by 150 publications

References 40 publications

Transcription Factors of Lotus: Regulation of Isoflavonoid Biosynthesis Requires Coordinated Changes in Transcription Factor Activity

Transcription Factors of Lotus: Regulation of Isoflavonoid Biosynthesis Requires Coordinated Changes in Transcription Factor Activity

NopL, an Effector Protein ofRhizobiumsp. NGR234, Thwarts Activation of Plant Defense Reactions

Regeneration of plants from callus cultures of roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi

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