High frequency electroporation efficiency is under control of membrane capacitive charging and voltage potential relaxation

Novickij, Vitalij; Ruzgys, Paulius; Grainys, Audrius; Šatkauskas, Saulius

doi:10.1016/j.bioelechem.2017.09.006

Cited by 47 publications

(45 citation statements)

References 51 publications

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“…Usually, a trade-off between the PEF amplitude and duration is made under a certain pulse repetition number, but the proper PEF application protocol is influenced by the cell type in general [21,22]. For instance, for mammalian cells, a well-established protocol is to apply a burst of microsecond pulses (e.g.…”

Section: Of 10mentioning

confidence: 99%

“…For instance, for mammalian cells, a well-established protocol is to apply a burst of microsecond pulses (e.g. 100 µs × 8) in the kV/cm PEF strength range [22,23]. However, in recent years, numerous researchers have started to focus on shorter (sub-microsecond) but higher intensity (range of kV) PEF pulses [24][25][26].…”

Section: Of 10mentioning

confidence: 99%

“…Additional interest into the use of high-voltage sub-microsecond PEFs lies in the possibility of inducing cell apoptosis [30,31]. However, one of the most particular focuses is in biomedical applications (e.g., improvement of electrochemotherapy and tissue ablation protocols) [22].…”

Section: Of 10mentioning

confidence: 99%

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Validation of a SPICE Model for High Frequency Electroporation Systems

2019

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In this paper, we present an analysis and a validation of a simulation program with integrated circuit emphasis (SPICE) model for a pulse forming circuit of a high frequency electroporation system, which can deliver square-wave sub-microsecond (100–900 ns) electric field pulses. The developed SPICE model is suggested for use in evaluation of transient processes that occur due to high frequency operations in prototype systems. A controlled crowbar circuit was implemented to support a variety of biological loads and to ensure a constant electric pulse rise and fall time during electroporation to be independent of the applied buffer bioimpedance. The SPICE model was validated via a comparison of the simulation and experimental results obtained from the already existing prototype system. The SPICE model results were in good agreement with the experimental results, and the model complexity was found to be sufficient for analysis of transient processes. As result, the proposed SPICE model can be useful for evaluation and compensation of transient processes in sub-microsecond pulsed power set-ups during the development of new prototypes.

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Section: Of 10mentioning

confidence: 99%

Section: Of 10mentioning

confidence: 99%

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Validation of a SPICE Model for High Frequency Electroporation Systems

2019

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“…However, most of the existing numerical and analytical studies have tackled the modelling of this phenomenon based on various assumptions and constraints to predict and evaluate cell and tissue EP. [1][2][3][4][5][6][7][8][9][10][11][12][13][14] Typically, EP takes place when the transmembrane potential (TMP) exceeds a threshold V ep above which electrically conductive pores start forming in the membrane. [1][2][3] Experimental estimates for V ep fall in the range of 0.5-1.2 V but theoretical estimates point to V ep ¼ 0.258 V. 15 Most of the existing studies have so far assumed that cell membranes are rigid.…”

mentioning

confidence: 99%

“…4 We also note that other authors have proposed interesting 3D models of simple or realistically shaped cells in densely packed tissues. [11][12][13][14]19,20 We use a model characterized by a principle of minimality in many respects. As in Refs.…”

mentioning

confidence: 99%

Assessing the electro-deformation and electro-poration of biological cells using a three-dimensional finite element model

Shamoon

Dermol–Černe

Rems

et al. 2019

Applied Physics Letters

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In this Letter, we explore how cell electro-deformation and electro-poration are connected. We build a time-domain model of layered concentric shells (a model of biological cells) including their dielectric and elastic properties. We simulate delivery of one trapezoidal voltage pulse to either a single spherical cell or an assembly of three neighboring cells in a specific configuration and calculate cell deformation and pore formation. We describe the qualitative features of the electric field, surface charge density, transmembrane voltage, cell elongation, and pore density distribution at specific times i.e., before, during and after the application of the electric pulse and explore the correlations between them. Our results show that (1) the polarization charge redistribution plays a significant role in the spatial distribution of electrical stresses at μs time scales and (2) the cell deformation and pore density can be correlated with regions of high surface charge density. In future work, our model could be used for understanding basic mechanisms of electro-deformation and electro-poration with high-frequency short bipolar pulses of biological cells in suspension or tissues.

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A generalized closed‐form analytic design technique for solid‐state Marx generators

Hassan,

Safwat,

Abounemra

et al. 2024

Circuit Theory & Apps

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SummarySolid‐state Marx generators (SSMGs) are capable of generating high‐voltage pulses, with certain repetition frequency, and have a multitude of applications. Published research work targets more elaborate SSMG circuits without the existence of a specific design procedure. In this paper, basic circuit parameters (stage capacitance, duty cycle and voltage droop) of generic resistive‐loaded SSMG—producing a single/burst of pulses—are precisely modeled. Accordingly, a generalized, closed‐form analytic design algorithm suitable for any resistive‐loaded SSMG is presented; the proposed algorithm guides designers throughout the SSMG design phase and furthermore, produces parametric design curves that provide designers with an in‐depth vision of the individual effect of each design parameter on the circuit performance. To quantify the robustness of the proposed algorithm, it is used to design a simple conventional, load‐dependent SSMG architecture fulfilling specific application requirements (frequency of 10 KHz, peak output voltage of 1.2 KV, and 510‐W average power per burst). Based on the obtained design and using the derived equations, the average power per burst is calculated for different number of pulses in the range of 1–10 pulses for the simulation and the implementation. The resulting relative error between the simulation and the measurement results is found to be in the range of 0.14%–0.37%. Afterward, the proposed algorithm is used to validate the published results of a complex SSMG. The relative error between the published results and those obtained using the proposed algorithm does not exceed 2.5%; this validates the modeling behind the proposed design technique and renders it as a solution approach for any resistive‐loaded SSMG topology irrespective of its complexity.

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High frequency electroporation efficiency is under control of membrane capacitive charging and voltage potential relaxation

Cited by 47 publications

References 51 publications

Validation of a SPICE Model for High Frequency Electroporation Systems

Validation of a SPICE Model for High Frequency Electroporation Systems

Assessing the electro-deformation and electro-poration of biological cells using a three-dimensional finite element model

A generalized closed‐form analytic design technique for solid‐state Marx generators

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