High fat high fructose diet induces mild oxidative stress and reorganizes intermediary metabolism in male mouse liver: Alpha-ketoglutarate effects

Bayliak, Maria M.; Vatashchuk, Myroslava; Gospodaryov, Dmytro V.; Hurza, Viktoria; Demianchuk, Oleh I.; IVANOCHKO, M.V.; Burdyliuk, Nadia; Storey, Kenneth B.; Lushchak, Oleh; Lushchak, Volodymyr I.

doi:10.1016/j.bbagen.2022.130226

Cited by 6 publications

(5 citation statements)

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“…In this context, the excess of energy substrates from different types of diet is associated with oxidative damage to macromolecules such as lipids (lipid peroxidation or lipid peroxidation) and proteins (carbonylation and/or nitration) because they increase the production of ROS, which alter the chemical structure and/or biological function, inducing cell death and consequent deleterious effects (Fernández-Sánchez et al, 2011). Bayliak et al (2022) have observed that male mice fed high-fat high-fructose diet (45% kcal fat, 15% kcal fructose) presented mild oxidative stress with higher activities of primary antioxidant enzymes namely CAT, glutathione peroxidase (GPx), and glutathione-S-transferase. In disagreement with the literature, our findings showed no significant differences between the experimental groups for the levels of MDA and carbonylated proteins in serum and adipose tissue, as well as, the activities of antioxidant enzymes (SOD and CAT) did not present alterations, demonstrating that dietary interventions did not lead to oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…Bayliak et al ( 2022 ) have observed that male mice fed high‐fat high‐fructose diet (45% kcal fat, 15% kcal fructose) presented mild oxidative stress with higher activities of primary antioxidant enzymes namely CAT, glutathione peroxidase (GPx), and glutathione‐ S ‐transferase. In disagreement with the literature, our findings showed no significant differences between the experimental groups for the levels of MDA and carbonylated proteins in serum and adipose tissue, as well as, the activities of antioxidant enzymes (SOD and CAT) did not present alterations, demonstrating that dietary interventions did not lead to oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

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High‐fat, high‐sucrose, and combined high‐fat/high‐sucrose diets effects in oxidative stress and inflammation in male rats under presence or absence of obesity

Kobi

Matias

Gasparini

et al. 2023

Physiological Reports

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

High‐fat, high‐sucrose, and combined high‐fat/high‐sucrose diets effects in oxidative stress and inflammation in male rats under presence or absence of obesity

Kobi

Matias

Gasparini

et al. 2023

Physiological Reports

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show abstract

“…HF diet induces metabolic syndrome and simple steatosis, usually not spontaneously progressing toward NASH unless an additional stimulus is provided [ 42 ]. The HFF diet induces liver pathology prior to, or even in the absence of, obesity or insulin resistance in association with skeletal muscle extracellular lipid accumulation, inflammation, and autophagy [ 43 ] and mild oxidative stress [ 44 ]. The CC diet was developed to mimic the cholesterol-mediated insulin resistance, cardiovascular risk increase, and NASH; the CC diet produces steatosis, inflammation, HSC activation, and fibrosis; however, animals fed with this diet are not insulin-resistant; and tend to lose weight and have lower triglyceride levels, with respect to standard chow-fed mice [ 2 ].…”

Section: Discussionmentioning

confidence: 99%

MCD Diet Modulates HuR and Oxidative Stress-Related HuR Targets in Rats

Ferrigno

Campagnoli

Barbieri

et al. 2023

IJMS

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The endogenous antioxidant defense plays a big part in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), a common metabolic disorder that can lead to serious complications such as cirrhosis and cancer. HuR, an RNA-binding protein of the ELAV family, controls, among others, the stability of MnSOD and HO-1 mRNA. These two enzymes protect the liver cells from oxidative damage caused by excessive fat accumulation. Our aim was to investigate the expression of HuR and its targets in a methionine-choline deficient (MCD) model of NAFLD. To this aim, we fed male Wistar rats with an MCD diet for 3 and 6 weeks to induce NAFLD; then, we evaluated the expression of HuR, MnSOD, and HO-1. The MCD diet induced fat accumulation, hepatic injury, oxidative stress, and mitochondrial dysfunction. A HuR downregulation was also observed in association with a reduced expression of MnSOD and HO-1. Moreover, the changes in the expression of HuR and its targets were significantly correlated with oxidative stress and mitochondrial injury. Since HuR plays a protective role against oxidative stress, targeting this protein could be a therapeutic strategy to both prevent and counteract NAFLD.

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“…Activities of hexokinase, phosphofructokinase, pyruvate kinase, lactate dehydrogenase, and glutamate dehydrogenase (GDH) were determined by monitoring NADP + reduction or NADH oxidation at 340 nm as described previously (Bayliak et al, 2022; Gospodaryov et al, 2020; Lylyk et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Alpha‐ketoglutarate supplementation in long‐lived Drosophila melanogaster: Impact on lifespan and metabolic responses

Demianchuk,

Lylyk,

Balatskiy

et al. 2024

Arch Insect Biochem Physiol

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Studies on antiaging remedies in insect models sometimes show discrepancies in results. These discrepancies could be explained by different responses of short‐ and long‐lived strains on the antiaging remedies. The purpose of the study was to test whether life‐prolonging effects of alpha‐ketoglutarate (AKG), observed in nematodes and fruit flies, would be reproduced in long‐lived Drosophila melanogaster flies. Lifespan was assayed in flies kept in demographic cages. Fecundity, proportion of flies capable of negative geotaxis, starvation resistance, time of heat coma onset, levels of triacyglycerols, body glucose, glycogen, activities of glutamate dehydrogenase, catalase, glutathione‐S‐transferase, hexokinase, phosphofructokinase, pyruvate kinase, lactate, and glutamate dehydrogenases were assessed. Dietary AKG did not affect fly lifespan on the diet with 5% yeast and 5% sucrose (5Y:5S) and on the diet with 9% yeast and 1% sucrose (9Y:1S), but increased lifespan on the low‐protein diet (1Y:9S). Twenty‐five‐day‐old female flies fed a 5Y:5S diet with 10 mM AKG for 3 weeks, did not differ from the control group (without AKG) in climbing activity, resistance to heat stress, and starvation. The levels of glucose and glycogen were unaffected but the levels of triacylglycerols were lower in AKG‐fed female flies. No differences in activities of glycolytic enzymes, NADPH‐producing enzymes, glutamate dehydrogenase, oxygen consumption, and levels of oxidative stress markers were observed between the control and AKG‐fed flies. However, AKG‐fed flies had lower activities of catalase and glutathione‐S‐transferase. These results suggest that potential antiaging remedies, such as AKG, may not extend lifespan in long‐living organisms despite influencing several metabolic parameters.

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High fat high fructose diet induces mild oxidative stress and reorganizes intermediary metabolism in male mouse liver: Alpha-ketoglutarate effects

Cited by 6 publications

References 58 publications

High‐fat, high‐sucrose, and combined high‐fat/high‐sucrose diets effects in oxidative stress and inflammation in male rats under presence or absence of obesity

High‐fat, high‐sucrose, and combined high‐fat/high‐sucrose diets effects in oxidative stress and inflammation in male rats under presence or absence of obesity

MCD Diet Modulates HuR and Oxidative Stress-Related HuR Targets in Rats

Alpha‐ketoglutarate supplementation in long‐lived Drosophila melanogaster: Impact on lifespan and metabolic responses

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