High efficiency transformation protocol for three Indian cotton varieties via Agrobacterium tumefaciens

Transgenic Indian Cotton (Gossypium hirsutum) Harboring Rice Chitinase Gene (Chi II) Confers Resistance to Two Fungal Pathogens

“…These results represent a convincing confirmation of Agrobacterium-mediated transformation of cotton and showed the potential reproducible protocol for genetic engineering of Indian cotton. The frequencies of transformation obtained in this study seem to be equal or slightly higher than other systems of transformation, although genotypic differences were observed [18] .…”

“…These observations indicated that the Chi II gene had been integrated into the genome of the transformed shoots of T 0 progeny thereby confirming transformation. Some reports proved that the shoot tip/meristem transformation protocols, albeit genotype-independent, are extremely laborious and generate a high frequency of chimeras [18,32] . Our experiments on PCR analysis proved that chimera formation significantly reduced and the randomly selected 6 plantlets showed PCR positive results.…”

“…Compared with somatic embryogenesis, the shoot tip mediated regeneration techniques are easy and less time-consuming process [12] . In recent years, there has been increasing focus on the use of meristems and shoot axes as the source of tissue explants for transgenic cotton production [13][14][15][16][17][18] . Hence, in the present investigation, we targeted to produce the cotton plantlets with chitinase gene through Agrobacteriummediated transformation protocol by using shoot tip culture technique.…”

Agrobacterium-mediated transformation of tomato (Lycopersicon esculentumL. cv. Hezuo 908) with improved efficiency

“…Kanamycin, widely used in plant transformation, is phytotoxic and inhibits the regeneration of untransformed plantlets. [21] After assessing the sensitivity of the explants, 50 mg/L kanamycin concentration was screened and selected for both explants (data not shown).…”

Plant Genetic Engineering: Problems and Applications

“…10.1 ). Advances have been made by using different transformation technologies like Agrobacterium-mediated transformation (Umbeck et al 1987 ;Firoozabady 1989 ;Cousins et al 1991 ;Gould and Magallanes 1998 ;Satyavathi et al 2002 ;Shuangxia et al 2005 ;Franklin et al 2008 ) , Gene Gun or combining both the methods together have also been reported (Firoozabady et al 1987 ;Christou et al 1989 ;Perlak et al 1990 ;McCabe and Martinell 1993 ;Rajasekaran et al 1996Rajasekaran et al , 2000Zapata et al 1999 ; . Sonication assisted transformation Finer 1997, 1998 ;da-Silva and Fukai 2002 ;Solís et al 2003 ;Jiang et al 2004 ;Rashid et al 2008 ) and Direct DNA uptake into protoplast (Khan and Maliga 1999 ;Daniell and Dhingra 2002 ) have also been used to which the researchers are required to get the reported literature/information.…”