High-density speckle contrast optical tomography (SCOT) for three dimensional tomographic imaging of the small animal brain

Abstract

High-density speckle contrast optical tomography (SCOT) utilizing tens of thousands of source-detector pairs, was developed for in vivo imaging of blood flow in small animals. The reduction in cerebral blood flow (CBF) due to local ischemic stroke in a mouse brain was transcanially imaged and reconstructed in three dimensions. The reconstructed volume was then compared with corresponding magnetic resonance images demonstrating that the volume of reduced CBF agrees with the infarct zone at twenty-four hours.

High-density speckle contrast optical tomography of cerebral blood flow response to functional stimuli in the rodent brain

“…27,28 Our recent developments in mice revealed comparable results to magnetic resonance imaging in following the infarct induced by CBF reduction. 27 A similar development 29 has been successfully used in the intraoperative room, where the three-dimensional (3-D) distribution of blood flow from a mastectomy skin flap was resolved. These applications highlight the potential of tomographic speckle contrast imaging in the clinic and in biomedical research.…”

“…25 SCOT can achieve scalability using the speckle contrast with an appropriate correlation diffusion model to relax the requirement, which the data-type used by DCT has, i.e., relatively fast (tens of nanoseconds) output from the detectors, thus allowing for readily available detector arrays to be utilized in the measurements. [25][26][27][28] Here we highlight speckle contrast optical tomography (SCOT) which, by taking advantage of the scalability of SCOS detection, achieves tomographic measurements using scanning sources and array detectors. 27,28 Our recent developments in mice revealed comparable results to magnetic resonance imaging in following the infarct induced by CBF reduction.…”

“…[25][26][27][28] Here we highlight speckle contrast optical tomography (SCOT) which, by taking advantage of the scalability of SCOS detection, achieves tomographic measurements using scanning sources and array detectors. 27,28 Our recent developments in mice revealed comparable results to magnetic resonance imaging in following the infarct induced by CBF reduction. 27 A similar development 29 has been successfully used in the intraoperative room, where the three-dimensional (3-D) distribution of blood flow from a mastectomy skin flap was resolved.…”

Diffuse correlation tomography in the transport regime: A theoretical study of the sensitivity to Brownian motion

“…Moreover, it is necessary to stress the importance of writing the sensitivity through kernels since the local dependence on position inside the medium is maintained (equivalently to DCT Jacobians while, the sensitivity as defined in this paper depends on the source and detector positions only). In addition, (17) takes into account the variation with respect to a static medium, i.e., satisfying the RTE. Thus, the sensitivity can be calculated by summing only onto the dynamic portion of the medium (i.e., the perturbation) and no modification of (11) is necessary in order to correct for scattering from the static part (as proposed in Ref.…”

“…It is based on the use of massively parallel detectors such as CMOS cameras. In some implementations, SCOT can utilize the degrees of freedom in the data characteristic of the transport regime in addition to those typically used in the diffuse regime [17].…”