“…In contrast to the his operons of S. typhimurium and E. coli, the his region of Klebsiella pneumoniae has not been extensively characterized, although it is likely that it will contain a his operon structurally and functionally similar to the analogous S. typhimurium and E. coli operons. The K. pneumoniae hisD and hisG genes, coding for enzymes involved in the last and first steps of the histidine biosynthetic pathway (Hartman et al 1971), as well as the his operon regulatory region, are located immediately adjacent to the nif (nitrogen fixation) gene cluster (Streicher et al, 1972;Dixon and Postgate, 1972;see Figure 1). K. pneumoniae DNA fragments carrying the hisD and hisG structural genes, the hisO(P) 'Present address: Department of Molecular Biology, Massachusetts General Hospital, Jackson Research Building, Boston, MA 02114.…”