2015
DOI: 10.1074/jbc.m115.650028
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Abstract: Background: Autophagy is required in hematopoiesis and protects against leukemogenesis. Results: When ATG7-dependent canonical autophagy is impaired, ATG7-independent alternative autophagy engages in myeloid cells but not in hematopoietic stem cells. Conclusion:The integrity of hematopoietic stem cells is jeopardized by a lack of alternative autophagy. Significance: Learning autophagy organization in hematopoietic system is crucial for understanding hematopoietic stem cell transformation.

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Cited by 33 publications
(36 citation statements)
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References 42 publications
(50 reference statements)
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“…These results are in contrast to the results of a recent study that reported that deletion of beclin 1 using TALEN disrupted autophagic flux by disrupting the formation of autophagosomes but not by disrupting LC3b lipidation in human Hela cells33. However, the present data appear to be consistent with our recent finding that somatic cells, but not stem cells, can acquire an alternative form of autophagy when their canonical autophagy pathway is disrupted3435.…”
Section: Resultssupporting
confidence: 60%
See 1 more Smart Citation
“…These results are in contrast to the results of a recent study that reported that deletion of beclin 1 using TALEN disrupted autophagic flux by disrupting the formation of autophagosomes but not by disrupting LC3b lipidation in human Hela cells33. However, the present data appear to be consistent with our recent finding that somatic cells, but not stem cells, can acquire an alternative form of autophagy when their canonical autophagy pathway is disrupted3435.…”
Section: Resultssupporting
confidence: 60%
“…S8C). These suggest that an alternative autophagy may be activated when beclin 1 is deleted because ULK1 was upregulated and recruited to autophagosome assembly site during activation of an alternative autophagy3435.…”
Section: Resultsmentioning
confidence: 96%
“…The intrinsic DNA cytidine deaminase activity of A3Fc-CD2 and its variants was measured by expressing these proteins in ung-deficient E. coli BW310 and by quantifying the frequency of Rif R -conferring rpoB mutations, [37,54] as described in the previous report. [39,42] Lots of single-base mutations in rpoB lead to activesite amino acid replacements which confer Rif R . In each case, five single colonies were grown at 37 o C in LB medium containing 100 μg /mL ampicillin, and induced overnight by 1 mM IPTG at 18 o C. Then appropriate volumes of cells were spread on plates containing 100 μg/mL rifampicin to select for Rif R mutants and to plates containing 100 μg/mL amplicillin to determine the number of viable cells.…”
Section: E Coli Based Deaminase Activity Assaymentioning
confidence: 99%
“…We therefore investigated whether there might be a TFEB‐independent/ alternative autophagy present in these cells. One of these pathways is thought to originate at the Golgi and is disrupted by Brefeldin A (BFA), a golgi inhibitor . We therefore examined whether BFA‐sensitive autophagy might contribute to the autophagy present during differentiation.…”
Section: Resultsmentioning
confidence: 99%
“…One of these pathways is thought to originate at the Golgi and is disrupted by Brefeldin A (BFA), a golgi inhibitor. 42 We therefore examined whether BFAsensitive autophagy might contribute to the autophagy present during differentiation. 3 and 7) or a combination of both (lanes 4 and 8).…”
Section: Evidence For Alternative Autophagymentioning
confidence: 99%