Hexokinase of Rabbit Lenses

A study of three enzymes acting on glucose in the lens of different species

“…of calf lens was found to be much higher (about threefold) than the hexokinase activity of cow lens. This result is in agreement with the findings of Green & Solomon (1959) that the hexokinase activity of young rabbit lens/g. of lens was about four times that of old rabbit lens.…”

“…1), but activity was not much lower even at pH 6 5 and at pH 8-0 (65% and 70% of maximum activity respectively). Green & Solomon (1959) reported that hexokinase in homogenates of lenses from young and old rabbits had maximum activity at pH 6-5-7-7 and 7-3-8 0 respectively. 100 -a .P4 6 7 8 9 lo pH Fig.…”

“…Three enzymes which act on glucose have been reported to be present in eye lens: hexokinase (Green & Solomon, 1959;Nordmann & Mandel, 1955;van Heyningen & Pirie, 1957); aldose reductase (van Heyningen, 1959a;Hayman & Kinoshita, 1965); glucose dehydrogenase ). The reactions catalysed by these enzymes are shown below:…”

“…2). Green & Solomon (1959) reported Km (glucose) values for hexokinase in extracts of young and old rabbit lenses of 039mM and O029 mm respectively. Hexokinases from animal tissues have been found to have Km (glucose) values in the range 0 1-0 001 mm, e.g.…”

A study of three enzymes acting on glucose in the lens of different species

“…of calf lens was found to be much higher (about threefold) than the hexokinase activity of cow lens. This result is in agreement with the findings of Green & Solomon (1959) that the hexokinase activity of young rabbit lens/g. of lens was about four times that of old rabbit lens.…”

“…1), but activity was not much lower even at pH 6 5 and at pH 8-0 (65% and 70% of maximum activity respectively). Green & Solomon (1959) reported that hexokinase in homogenates of lenses from young and old rabbits had maximum activity at pH 6-5-7-7 and 7-3-8 0 respectively. 100 -a .P4 6 7 8 9 lo pH Fig.…”

“…Three enzymes which act on glucose have been reported to be present in eye lens: hexokinase (Green & Solomon, 1959;Nordmann & Mandel, 1955;van Heyningen & Pirie, 1957); aldose reductase (van Heyningen, 1959a;Hayman & Kinoshita, 1965); glucose dehydrogenase ). The reactions catalysed by these enzymes are shown below:…”

“…2). Green & Solomon (1959) reported Km (glucose) values for hexokinase in extracts of young and old rabbit lenses of 039mM and O029 mm respectively. Hexokinases from animal tissues have been found to have Km (glucose) values in the range 0 1-0 001 mm, e.g.…”

A study of three enzymes acting on glucose in the lens of different species

“…of calf lens was found to be much higher (about threefold) than the hexokinase activity of cow lens. This result is in agreement with the findings of Green & Solomon (1959) that the hexokinase activity of young rabbit lens/g. of lens was about four times that of old rabbit lens.…”

“…1), but activity was not much lower even at pH 6 5 and at pH 8-0 (65% and 70% of maximum activity respectively). Green & Solomon (1959) reported that hexokinase in homogenates of lenses from young and old rabbits had maximum activity at pH 6-5-7-7 and 7-3-8 0 respectively. 100 -a .P4 6 7 8 9 lo pH Fig.…”

“…Three enzymes which act on glucose have been reported to be present in eye lens: hexokinase (Green & Solomon, 1959;Nordmann & Mandel, 1955;van Heyningen & Pirie, 1957); aldose reductase (van Heyningen, 1959a;Hayman & Kinoshita, 1965); glucose dehydrogenase ). The reactions catalysed by these enzymes are shown below:…”

“…2). Green & Solomon (1959) reported Km (glucose) values for hexokinase in extracts of young and old rabbit lenses of 039mM and O029 mm respectively. Hexokinases from animal tissues have been found to have Km (glucose) values in the range 0 1-0 001 mm, e.g.…”

A study of three enzymes acting on glucose in the lens of different species

“…of calf lens was found to be much higher (about threefold) than the hexokinase activity of cow lens. This result is in agreement with the findings of Green & Solomon (1959) that the hexokinase activity of young rabbit lens/g. of lens was about four times that of old rabbit lens.…”

“…1), but activity was not much lower even at pH 6 5 and at pH 8-0 (65% and 70% of maximum activity respectively). Green & Solomon (1959) reported that hexokinase in homogenates of lenses from young and old rabbits had maximum activity at pH 6-5-7-7 and 7-3-8 0 respectively. 100 -a .P4 6 7 8 9 lo pH Fig.…”

“…Three enzymes which act on glucose have been reported to be present in eye lens: hexokinase (Green & Solomon, 1959;Nordmann & Mandel, 1955;van Heyningen & Pirie, 1957); aldose reductase (van Heyningen, 1959a;Hayman & Kinoshita, 1965); glucose dehydrogenase ). The reactions catalysed by these enzymes are shown below:…”

“…2). Green & Solomon (1959) reported Km (glucose) values for hexokinase in extracts of young and old rabbit lenses of 039mM and O029 mm respectively. Hexokinases from animal tissues have been found to have Km (glucose) values in the range 0 1-0 001 mm, e.g.…”