Heterotrophic growth and oil production from Micractinium sp. ME05 using molasses

Engin, Iskin Kose; Çekmecelioğlu, Deniz; Yücel, Ayşe Meral; Öktem, Hüseyin Avni

doi:10.1007/s10811-018-1486-2

Cited by 7 publications

(3 citation statements)

References 58 publications

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“…Environmental elements, including factors like photoperiod and light intensity, play a crucial role in the growth of algae. However, their influence varies among species and is contingent upon the specific product under investigation [67,68]. Additionally, light significantly impacts the growth and morphogenesis of callus by influencing the rate of cell division in various plants, directly regulating plant growth and development [69].…”

Section: Effect Of Photoperiod and Temperaturementioning

confidence: 99%

Establishment of Effective Callus Induction in the Economically Important Brown Seaweed Ecklonia cava

Lee,

Bashir,

Tirtawijaya

et al. 2024

Applied Sciences

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The edible brown seaweed, Ecklonia cava, is highly valued for its bioactive compounds, and is widely used in food supplements and functional foods. The increasing demand for this seaweed in the food industry emphasizes the necessity for sustainable cultivation practices. This study focused on inducing callus in the meristem and stipe of E. cava using different culture media: Provasoli’s enriched seawater medium (PESI), enriched artificial seawater medium (ESAW), artificial enriched seawater medium (ASP2), or Von Stosch’s enriched seawater medium (VS). Various abiotic stress factors (photoperiod, agar concentration, and temperature), growth regulators, carbon sources, polyamines, and plasma treatments were explored for their impact on callus induction. Both stipe and meristem explants developed callus within three to six weeks across all media except ASP2. Callus development was favored at temperatures between 8 to 13 °C and in the absence of light. Stipe explants showed a higher callus induction rate (up to 65.59 ± 6.24%) compared to meristem (up to 57.53 ± 8.32%). Meristem explants showed optimal callus induction in PESI medium with a low concentration of indole-3-acetic acid (IAA; 40.93 ± 8.65%). However, higher concentrations of IAA and 1-naphthaleneacetic acid (NAA) reduced meristem callus induction. Stipe showed high induced-callus (up to 50.37 ± 5.17%) in PESI medium with low concentrations of IAA, NAA, and 6-benzylaminopurine (BAP). Both stipe and meristem explants induced largest callus at 2% sucrose, but higher carbon source concentrations reduced callus induction. Spermine (Spm) at 1 µM resulted in high induced calluses; however, increasing Spm concentrations decreased callus induction. This tissue culture technique not only supports mass cultivation of E. cava, but also holds potential for extending to other seaweed species, contributing to the sustainability of seaweed stocks for the food industry.

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Section: Effect Of Photoperiod and Temperaturementioning

confidence: 99%

Establishment of Effective Callus Induction in the Economically Important Brown Seaweed Ecklonia cava

Lee,

Bashir,

Tirtawijaya

et al. 2024

Applied Sciences

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“…into 1 L Bold's Basal Medium (BBM) supplemented with 19 g of molasses hydrolysate in 2-L Erlenmeyer asks. The composition of BBM and molasses, preparation of molasses hydrolysate and the optimum molasses hydrolysate amount for the highest biomass concentration were previously described by Engin et al (2018a). Micractinium sp.…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

“…METUNERGY05 (ME05), used in this study, was previously isolated in our laboratory from hot springs of Haymana, Turkey (Onay et al 2014). It is a thermotolerant strain which is suitable for biodiesel production and can be cultivated both mixotrophically and heterotrophically using by-products of a sugar factory as sole carbon source, which reduces the operational costs (Engin et al 2018a;Engin et al 2018b).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity of Micractinium sp. (Chlorophyta) extracts against H2O2 induced oxidative stress in human breast adenocarcinoma cells

Bulut,

Engin,

Sonmez

et al. 2024

Preprint

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In response to the growing demand for high-value bioactive compounds, microalgae cultivation has gained a significant acceleration in recent years. Among these compounds, antioxidants have emerged as essential constituents in the food, pharmaceutical, and cosmetics industries. This study focuses on Micractinium sp. ME05, a green microalgal strain previously isolated from hot springs flora in our laboratory. Micractinium sp. cells were extracted using six different solvents, and their antioxidant capacity, as well as total phenolic, flavonoid, and carotenoid contents, were evaluated. The methanolic extracts demonstrated the highest antioxidant capacity, measuring 7.72 and 93.80 µmol trolox equivalents.g-1 dry weight (DW) according to the DPPH and FRAP assays, respectively. To further characterize the biochemical profile, reverse phase high-performance chromatography (RP-HPLC) was employed to quantify twelve different phenolics, including rutin, gallic acid, benzoic acid, cinnamic acid, and β-carotene, in the microalgal extracts. Notably, the acetone extracts of Micractinium sp. grown mixotrophically contained a high amount of gallic acid (469.21 ± 159.74 µg.g-1 DW), while 4-hydroxy benzoic acid (403.93 ± 20.98 µg.g-1 DW) was the main phenolic compound in the methanolic extracts under heterotrophic cultivation. Moreover, extracts from Micractinium sp. exhibited remarkable cytoprotective activity by effectively inhibiting hydrogen peroxide-induced oxidative stress and cell death in human breast adenocarcinoma (MCF-7) cells. In conclusion, with its diverse biochemical composition and adaptability to different growth regimens, Micractinium sp. emerges as a robust candidate for mass cultivation in nutraceutical and food applications.

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Effect of organic carbon sources and environmental factors on cell growth and lipid content of Pavlova lutheri

et al. 2019

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Heterotrophic growth and oil production from Micractinium sp. ME05 using molasses

Cited by 7 publications

References 58 publications

Establishment of Effective Callus Induction in the Economically Important Brown Seaweed Ecklonia cava

Establishment of Effective Callus Induction in the Economically Important Brown Seaweed Ecklonia cava

Antioxidant activity of Micractinium sp. (Chlorophyta) extracts against H2O2 induced oxidative stress in human breast adenocarcinoma cells

Effect of organic carbon sources and environmental factors on cell growth and lipid content of Pavlova lutheri

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