Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants

Jiang, Yuanzhong; Ye, Sheng‐Long; Wang, Lijun; Duan, Yumin; Lu, Wei; Liu, Hong; Fan, Di; Zhang, Faqi; Luo, Keming

doi:10.1007/s11240-013-0393-0

Cited by 29 publications

(31 citation statements)

References 49 publications

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“…Due to the low stable transformation efficiency of most Malus species, the application of the VIGS system offers an attractive alternative for characterization of gene function without stable transformation (Grønlund et al 2008;Holzberg et al 2002;Liu et al 2002;Ratcliff et al 2001). The TRV vector is the most widely used VIGS vector and is effective for the transformation of many species, including some woody plants (Jiang et al 2014b;Ye et al 2009), as the virus has a broad host range (Bachan and Dinesh-Kumar 2012).…”

Section: Discussionmentioning

confidence: 99%

“…In tomato, recombinant TRV can efficiently spread and replicate in tomato fruit after either injection or vacuum infiltration (Fu et al 2005). However, since vacuum infiltration has been reported to result in more effective gene silencing in Gerbera hybrid (Deng et al 2012), opium poppy (Papaver somniferum; Hileman et al 2005), Thalictrum (Di Stilio et al 2010) and strawberry (Tian et al 2014), we reasoned that it might also be suitable for VIGS in younger seedlings and woody plants (Jiang et al 2014b;Yan et al 2012). Temperature influences several aspects of VIGS efficiency, and TRV mediated VIGS is sensitive to high temperatures.…”

Section: Discussionmentioning

confidence: 99%

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An optimized TRV-based virus-induced gene silencing protocol for Malus crabapple

Zhang

Tian

Tai

et al. 2016

Plant Cell Tiss Organ Cult

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Virus-induced gene silencing (VIGS) is a valuable reverse genetics tool to study gene function in a broad range of plants. As the efficiency of apple stable transformation is very low and it is still not easy to produce a large number of apple transgenic plants for most laboratories. Here, we developed an easy and effective transient transformation method of agro-inoculation for virus-induced gene silencing based on vacuum infiltration in genus of apples, the crabapple. The entire young tissue cultured crabapple plantlets were used as the plant material for infiltration, and a -90 kPa vacuum pressure and 19°C day/ 18°C night cultivation temperature were shown to result in highest VIGS efficiency. Infiltration with tobacco rattle virus containing a fragment of the transcription factor gene McMYB10, which regulates the synthesis of anthocyanin pigments, resulted in low levels of McMYB10 transcripts and characteristic faded red leaves. In addition, the expression of several anthocyanin biosynthetic genes was down-regulated in the transformed leaves. The results of this study indicate that the MYB10 gene represents a useful reporter gene for VIGS in crabapple and that the VIGS technique can be successfully applied to crabapple, thereby providing a platform for functional genetic studies in this and likely other Malus species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

An optimized TRV-based virus-induced gene silencing protocol for Malus crabapple

Zhang

Tian

Tai

et al. 2016

Plant Cell Tiss Organ Cult

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“…Virus-induced gene silencing (VIGS) is a powerful technology that uses engineered viruses to specifically silence host gene expression through post-transcriptional gene silencing (Becker & Lange 2010;Krishnan et al 2015;Purkayastha & Dasgupta 2009). VIGS is an effective method for the large-scale analysis of genes and their functions, and it has been successfully performed in many plants, including tobacco, Arabidopsis, tomato, cotton, wheat, and many woody plants (Burch-Smith et al 2006;Jiang et al 2014;Kumagai et al 1995;Orzaez et al 2009;Scofield et al 2005). VIGS works via a mechanism that is similar to that of RNA interference (Baulcombe 1999;Waterhouse et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…VIGS works via a mechanism that is similar to that of RNA interference (Baulcombe 1999;Waterhouse et al 2001). Double-stranded (ds) RNA is the key to the VIGS process; the dsRNA is cleaved into short interfering (si) RNAs of 21 to 25 nucleotides (Jiang et al 2014). Two strands can be obtained from the siRNAs, the guide and passenger strands.…”

Section: Introductionmentioning

confidence: 99%

“…RNA 1 encodes genes with viral replication and movement functions, while RNA 2 encodes the coat protein and some nonessential structural proteins that can be replaced by foreign sequences (Hayward et al 2011). The TRV vector has been used in Gossypium spp., Arabidopsis and Vernicia fordii to silence the cloroplastos alterados 1 (CLA1) gene, which is involved in chloroplast development (Jiang et al 2014;Manhães et al 2015;Mustafa et al 2016). Silencing CLA1 is a useful marker for determining the silencing efficiency because of the bleached phenotype (Mustafa et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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Efficient virus-induced gene silencing in Hibiscus hamabo Sieb. et Zucc. using Tobacco rattle virus

Wang

et al. 2019

Preprint

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Background. Hibiscus hamabo Sieb. et Zucc. is a semi-mangrove plant used for the ecological restoration of saline-alkali land, coastal afforestation and urban landscaping. Because the genetic transformation of H. hamabo is difficult, molecular breeding and gene functional studies have been severely restricted. In plants, virus-induced gene silencing provides a pathway to rapidly and effectively create targeted gene knockouts for gene functional studies. Methods. In this study, we tested the efficiency of a Tobacco rattle virus vector in silencing the cloroplastos alterados 1 (CLA1) gene through agroinfiltration. Results. The leaves of H. hamabo showed white streaks typical of CLA1 gene silencing three weeks after agroinfiltration. In agroinfiltrated H. hamabo plants, the CLA1 expression levels in leaves with white streaks were all significantly lower than in those of mock-infected and control plants. Conclusions. Thus, this virus-induced gene silencing system was efficient in H. hamabo and may be a powerful tool for large-scale reverse-genetic analyses of gene functions in H. hamabo.

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Virus-Induced Gene Silencing in Olive Tree (Oleaceae)

Κουδούνας

Thomopoulou

Angeli

et al. 2020

Methods in Molecular Biology

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Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants

Cited by 29 publications

References 49 publications

An optimized TRV-based virus-induced gene silencing protocol for Malus crabapple

An optimized TRV-based virus-induced gene silencing protocol for Malus crabapple

Efficient virus-induced gene silencing in Hibiscus hamabo Sieb. et Zucc. using Tobacco rattle virus

Virus-Induced Gene Silencing in Olive Tree (Oleaceae)

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