Heterologous fibrin biopolymer associated to a single suture stitch enables the return of neuromuscular junction to its mature pattern after peripheral nerve injury

Pinto, Carina Guidi; Leite, Ana Paula; Sartori, Arthur Alves; Tibúrcio, Felipe Cantore; Barraviera, Benedito; Ferreira, Rui Seabra; Filadelpho, André Luís; Carvalho, Samara Camaçarí de; Matheus, Selma Maria Michelin

doi:10.1016/j.injury.2020.10.070

Cited by 9 publications

(19 citation statements)

References 54 publications

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“…Such findings are in line with studies that used FBP for the same purpose, obtaining favorable results in tissue regeneration [ 12 , 46 ]. The proportion of its components and the sequence of manipulation is adjusted according to the purpose of its use, for example, in the repair of nerve injuries [ 47 ], bone [ 14 , 48 , 49 ], venous ulcers [ 50 , 51 ], and stem cells [ 46 , 52 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

Photobiomodulation Therapy on the Guided Bone Regeneration Process in Defects Filled by Biphasic Calcium Phosphate Associated with Fibrin Biopolymer

et al. 2021

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The aim is to evaluate the effects of photobiomodulation therapy (PBMT) on the guided bone regeneration process (GBR) in defects in the calvaria of rats filled with biphasic calcium phosphate associated with fibrin biopolymer. Thirty male Wistar rats were randomly separated: BMG (n = 10), defects filled with biomaterial and covered by membrane; BFMG (n = 10), biomaterial and fibrin biopolymer covered by membrane; and BFMLG (n = 10), biomaterial and fibrin biopolymer covered by membrane and biostimulated with PBMT. The animals were euthanized at 14 and 42 days postoperatively. Microtomographically, in 42 days, there was more evident bone growth in the BFMLG, limited to the margins of the defect with permanence of the particles. Histomorphologically, an inflammatory infiltrate was observed, which regressed with the formation of mineralized bone tissue. In the quantification of bone tissue, all groups had a progressive increase in new bone tissue with a significant difference in which the BFMLG showed greater bone formation in both periods (10.12 ± 0.67 and 13.85 ± 0.54), followed by BFMG (7.35 ± 0.66 and 9.41 ± 0.84) and BMG (4.51 ± 0.44 and 7.11 ± 0.44). Picrosirius-red staining showed greater birefringence of collagen fibers in yellow-green color in the BFMLG, showing more advanced bone maturation. PBMT showed positive effects capable of improving and accelerating the guided bone regeneration process when associated with biphasic calcium phosphate and fibrin biopolymer.

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Section: Discussionmentioning

confidence: 99%

Photobiomodulation Therapy on the Guided Bone Regeneration Process in Defects Filled by Biphasic Calcium Phosphate Associated with Fibrin Biopolymer

et al. 2021

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“…The heterologous fibrin sealant, also known as fibrin biopolymer, is a genuinely Brazilian product derived from animals, biocompatible, and of low production cost in addition to adhesive, sealant, hemostatic, scaffold, and drug delivery properties [27][28][29][30][31][32][33]. This new fibrin sealant was evaluated in phase I/II clinical trial and have showed be safe, non-immunogenic in addition to good preliminary efficacy [34,35].…”

Section: Introductionmentioning

confidence: 99%

A biocomplex to repair experimental critical size defects associated with photobiomodulation therapy

Buchaim

Andreo

Pomini

et al. 2022

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: The association of scaffolds to repair extensive bone defects can contribute to their evolution and morphophysiological recomposition. The incorporation of particulate biomaterials into three-dimensional fibrin bioproducts together with photobiomodulation therapy (PBM) has potential and can improve regenerative medicine procedures. The objective of this experiment was to evaluate the effects of PBM therapy on critical size defects filled with xenogenic bone substitute associated with fibrin biopolymer. Methods: A critical defect of 8 mm was performed in 36 Wistar male adult rats that were divided into four groups. Groups BC and BC-PBM were defined as controls with defects filled by a clot (without or with PBM, respectively) and groups XS and XS-PBM that comprised those filled with biocomplex Bio-Oss TM in association with fibrin biopolymer. PBM was applied immediately after the surgery and three times a week every other day, with the parameters: wavelength of 830 nm, energy density 6.2 J/cm 2 , output power 30 mW, beam area of 0.116 cm 2 , irradiance 0.258,62 W/cm 2 , energy/point 0.72 J, total energy 2.88 J. Fourteen and 42 days after the surgery, animals were euthanatized and subjected to microtomography, qualitative and quantitative histological analysis.

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“…16 New approaches have been used in addition to suturing to improve nerve regeneration, including adjuvants such as heterologous fibrin biopolymer (HFB), formerly called fibrin glue or heterologous fibrin sealant. 8,[17][18][19] It is a natural, biodegradable, bioabsorbable, and nontoxic biopharmaceutical with excellent adhesive capacity. 17,[19][20][21] HFB consists of a thrombin-like enzyme extracted from Crotalus durissus terrificus venom and a cryoprecipitate rich in fibrinogen from the blood of buffaloes (Bubalus bubalis).…”

Section: Introductionmentioning

confidence: 99%

“…17,20,22 It is a unique heterologous fibrin worldwide, as it does not use human blood, does not transmit infectious diseases, and can be used in several surgical procedures, especially peripheral nerve repair. 1,8,9,17,20,[23][24][25] Several biopolymers induce the activation of M2 macrophages, meaning that they can intensify the anti-inflammatory response and facilitate tissue remodeling. 2,[26][27][28] Thus, modulation of the immune response may be an efficient approach for improving neuroregeneration.…”

Section: Introductionmentioning

confidence: 99%

Neuroregeneration and immune response after neurorrhaphy are improved with the use of heterologous fibrin biopolymer in addition to suture repair alone

et al. 2023

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Introduction/Aims: Peripheral nerve injuries result in impaired neuromuscular interactions, leading to morphological and functional alterations. Adjuvant suture repair methods have been used to improve nerve regeneration and modulate the immune response. Heterologous fibrin biopolymer (HFB), a scaffold with adhesive properties, plays a critical role in tissue repair. The aim of this study is to evaluate neuroregeneration and immune response focusing on neuromuscular recovery, using sutureassociated HFB for sciatic nerve repair.Methods: Forty adult male Wistar rats were distributed into four groups (n = 10): C (control), only sciatic nerve location; D (denervated), neurotmesis and 6-mm gap removal and fixation stumps in subcutaneous tissue; S (suture), neurotmesis followed by suture; and SB (suture + HFB), neurotmesis followed by suture and HFB. Analysis of M2 macrophages (CD206 + ), as well as the morphology and morphometry of nerves, soleus muscle, and neuromuscular junctions (NMJs), were performed at 7 and 30 days after surgery. Results:The SB group had the highest M2 macrophage area in both periods. After 7 days, SB was the only group similar to the C group regarding the number of axons; furthermore, after 30 days, the SB group was closer to the C group concerning blood vessel and central myonuclear numbers, NMJ angle, and connective tissue volume.After 7 days, increases in nerve area, as well as the number and area of blood vessels, were also observed in SB.Discussion: HFB potentiates the immune response, increases axonal regeneration, induces angiogenesis, prevents severe muscle degeneration, and assists in NMJ recovery. In conclusion, suture-associated HFB has major implications for improved peripheral nerve repair.

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Heterologous fibrin biopolymer associated to a single suture stitch enables the return of neuromuscular junction to its mature pattern after peripheral nerve injury

Cited by 9 publications

References 54 publications

Photobiomodulation Therapy on the Guided Bone Regeneration Process in Defects Filled by Biphasic Calcium Phosphate Associated with Fibrin Biopolymer

Photobiomodulation Therapy on the Guided Bone Regeneration Process in Defects Filled by Biphasic Calcium Phosphate Associated with Fibrin Biopolymer

A biocomplex to repair experimental critical size defects associated with photobiomodulation therapy

Neuroregeneration and immune response after neurorrhaphy are improved with the use of heterologous fibrin biopolymer in addition to suture repair alone

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