2003
DOI: 10.1590/s0036-46652003000500005
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: SUMMARYStrongyloides ratti larval extract was used for the standardization of ELISA to detect genus-specific IgE in human strongyloidiasis. Forty serum samples from monoinfected patients shedding S. stercoralis larvae (Group I), 40 from patients with other intestinal parasites (Group II), and 40 from copronegative healthy subjects (Group III) were analyzed. Genus-specific IgE levels (ELISA Index: EI) were significantly higher in the group I (EI = 1.43) than groups II (EI = 0.70) and III (EI = 0.71), showing po… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
9
0
11

Year Published

2004
2004
2021
2021

Publication Types

Select...
7
2
1

Relationship

1
9

Authors

Journals

citations
Cited by 20 publications
(20 citation statements)
references
References 26 publications
0
9
0
11
Order By: Relevance
“…ELISA-IgE -Strongyloides-specific IgE antibodies were measured in serum samples of groups I, II, and III by indirect ELISA using heterologous antigen as described by Costa-Cruz et al (2003). Preliminary experiments were carried out in order to determine the optimal conditions for ELISA-IgE, through block titration of the reagents (antigen, sera, and conjugate).…”
Section: Methodsmentioning
confidence: 99%
“…ELISA-IgE -Strongyloides-specific IgE antibodies were measured in serum samples of groups I, II, and III by indirect ELISA using heterologous antigen as described by Costa-Cruz et al (2003). Preliminary experiments were carried out in order to determine the optimal conditions for ELISA-IgE, through block titration of the reagents (antigen, sera, and conjugate).…”
Section: Methodsmentioning
confidence: 99%
“…To overcome stool method limitations, serological diagnosis is often used. Nevertheless, false‐positive reactions caused by past infections or cross‐reactivity with other intestinal helminths overestimate the true prevalence of S. stercoralis infection, representing a great limitation for serologic assays . Specific IgG and IgE responses during human strongyloidiasis have been used for diagnostics, primarily for epidemiological studies and to support parasitological diagnosis.…”
Section: Introductionmentioning
confidence: 99%
“…Uma alternativa é a utilização de antígenos heterólogos, originados de larvas de outras espécies de Strongyloides, principalmente S. ratti e S. venezuelensis, devido às suas similaridades antigênicas com o Strongyloides stercoralis e à maior facilidade de obtenção das larvas em infecção experimental (COSTA-CRUZ et al, 2003;SILVA et al, 2003;MACHADO et al, 2003;SUDRÉ, 2006). Entretanto, Gam, Neva e Krotoski (1987) e Lindo e colaboradores (1994) relataram que a utilização de antígenos de S. stercoralis apresenta uma melhor sensibilidade quando comparada à dos antígenos de S. ratti ou S. venezuelensis no diagnóstico da infecção através do ELISA.…”
Section: Discussionunclassified