Heterogeneity of Lactobacillus plantarum isolates from Feta cheese throughout ripening

Xanthopoulos, V.; Hatzikamari, Magdalini; Adamidis, Trifon; Tsakalidou, Effie; Tzanetakis, N.; Litopoulou-Tzanetaki, E.

doi:10.1046/j.1365-2672.2000.01056.x

Cited by 53 publications

(35 citation statements)

References 18 publications

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“…In contrast, the acidifying rate of isolates after 24 h of incubation was lower than those reported in the former studies. The possibility to find groups of strains characterized by different acidifying ability is frequent in L. plantarum species (49). …”

Section: Resultsmentioning

confidence: 99%

Characterization of Lactobacillus from Algerian goat's milk based on phenotypic, 16S rDNA sequencing and their technological properties

Marroki¹,

Zúñiga²,

Kihal³

et al. 2011

Braz. J. Microbiol.

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Nineteen strains of Lactobacillus isolated from goat’s milk from farms in north-west of Algeria were characterized. Isolates were identified by phenotypic, physiological and genotypic methods and some of their important technological properties were studied. Phenotypic characterization was carried out by studying physiological, morphological characteristics and carbohydrate fermentation patterns using API 50 CHL system. Isolates were also characterized by partial 16S rDNA sequencing. Results obtained with phenotypic methods were correlated with the genotypic characterization and 13 isolates were identified as L. plantarum, two isolates as L. rhamnosus and one isolate as L. fermentum. Three isolates identified as L. plantarum by phenotypic characterization were found to be L. pentosus by the genotypic method. A large diversity in technological properties (acid production in skim milk, exopolysaccharide production, aminopeptidase activity, antibacterial activity and antibiotic susceptibility) was observed. Based on these results, two strains of L. plantarum (LbMS16 and LbMS21) and one strain of L. rhamnosus (LbMF25) have been tentatively selected for use as starter cultures in the manufacture of artisanal fermented dairy products in Algeria.

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Section: Resultsmentioning

confidence: 99%

Characterization of Lactobacillus from Algerian goat's milk based on phenotypic, 16S rDNA sequencing and their technological properties

Marroki¹,

Zúñiga²,

Kihal³

et al. 2011

Braz. J. Microbiol.

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“…The Lactobacillus paracasei DC205 and DC412 12 and Saccharomyces boulardii KK1 13 strains were obtained from the collection of the Laboratory of Food Microbiology and Hygiene of Aristotle University of Thessaloniki-Greece. All lactobacilli were stored at À801C in MRS broth with 25% glycerol, and KK1 cultures were maintained at 41C in a YEGP agar slope.…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Gluten induces coeliac-like disease in sensitised mice involving IgA, CD71 and transglutaminase 2 interactions that are prevented by probiotics

Papista

Gerakopoulos

Kourelis

et al. 2012

Laboratory Investigation

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Coeliac disease (CD) is a malabsorptive enteropathy resulting from intolerance to gluten. Environmental factors and the microbiota are suggested to have critical roles in the onset of CD. The CD71 IgA receptor on epithelial cells is responsible for abnormal retrotranscytosis of IgA-gluten peptide complexes from the intestinal lumen into the lamina propria, inducing intestinal inflammation. However, understanding the role of gluten in the CD physiopathology has been hindered by the absence of relevant animal models. Here, we generated a mouse model for CD to study the factors controlling its pathogenesis as well as to investigate the influence of oral delivery of probiotics on disease development. Gluten sensitivity was established by feeding three generations of BALB/c mice a gluten-free diet (GÀ) followed by gluten challenge (G þ ) for 30 days. The G þ mice developed villous atrophy, crypt hyperplasia and infiltration of T cells and macrophages in the small intestine. Inflammation was associated with an overexpression of CD71 on the apical side of enterocytes and an increase of plasma cells producing IgA, which colocalised with the CD71. Moreover, IgA colocalised with the transglutaminase 2 (TG2), the production of which was increased in the lamina propria of G þ mice. These mice displayed increased production of cyclooxygenase-2 (COX-2), pro-inflammatory cytokines and IL-15, as well as anti-gliadin and anti-TG2 autoantibodies. The commensal flora-isolated presumptive probiotic Saccharomyces boulardii KK1 strain hydrolysed the 28-kDa a-gliadin fraction, and its oral delivery in G þ mice improved enteropathy development in association with decrease of epithelial cell CD71 expression and local cytokine production. In conclusion, the G þ BALB/c mouse represents a new mouse model for human CD based on histopathological features and expression of common biomarkers. The selected probiotic treatment reversing disease development will allow the study of the role of probiotics as a new therapeutic approach of CD.

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“…Our results were in accordance with those of Ruiz-Barba et al (1991) who showed that 35 L. plantarum strains, isolated from different green olive fermentors, contained a large number of plasmids in the CCC form (from 5 to 16) ranging from 2.0 to 68 kb in size. Xanthopoulos et al (2000) also have found different plasmids in several L. plantarum strains isolated from Feta cheese. Delgado and Mayo (2003) have reported that L. plantarum LL441 strain harbours seven plasmids and produces a bacteriocin.…”

Section: Resultsmentioning

confidence: 81%

Plasmid DNA studies in <i>Lactobacillus plantarum</i> strains isolated from olive fermentations: production of and immunity to plantaricin OL15 is associated to a 9.6 Kb plasmid (pOL15)

Mourad¹

2007

Grasas y Aceites

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RESUMENEstudios de ADN plásmido en cepas de Lactobacillus plantarum aisladas de aceitunas fermentadas: La producción de y la inmunidad a plantaricina OL15 está asociada a un plásmido de 9.6 Kb (pOL15).En un estudio previo, se aislaron 12 cepas de Lactobacillus plantarum a partir de aceitunas fermentadas. Entre ellas, solo L. plantarum OL15 produjo bacteriocinas (plantaricin OL15). En este estudio, se examinó el contenido de AND plásmido en las 12 cepas citadas. Entre ellas, 9 cepas han mostrado de una a tres bandas de plásmido con tamaños en el rango de 5.4 a 12.2 kb. L. plantarum OL15 exhibió un plásmido (9.6 kb) que se denominó pOL15. Después del curado con novobiocina y bromuro de etidio, la pérdida del plásmido pOL15 asociada a la pérdida de su facultad para producir plantaricin OL15, sugiere que la producción de plantaricina OL15 está ligada al plásmido. La plantaricin OL15 no se inactivó por amilasa ni por lipasa sugiriendo que su actividad no es dependiente de la presencia de carbohidratos o lípidos. La plantaricina OL15 mostró actividad frente a bacterias lácticas de diferentes especies y también frente a residuos de aceitunas y bacterias fitopatogénicas, incluyendo Pseudomonas y Erwinia PALABRAS-CLAVE: Aceitunas -Agentes de curadoBacteriocinas -Lactobacillus plantarum -Plásmido. SUMMARYPlasmid DNA studies in Lactobacillus plantarum strains isolated from olive fermentations: production of and immunity to plantaricin OL15 is associated to a 9.6 Kb plasmid (pOL15).Previously 12 Lactobacillus plantarum strains were isolated from fermented olives. Among these, only L. plantarum OL15 produced bacteriocin (plantaricin OL15). In this study, the 12 strains were examined for plasmid DNA content. Of these, 9 strains have shown one to three plasmid bands ranging in size from 5.4 to 12.2 kb. L. plantarum OL15 exhibited one plasmid (9.6 kb) which was named pOL15. After curing with novobiocin and ethidium bromide, the plasmid profile analysis of non producing derivatives, showed that the 9.6 kb plasmid pOL15 harbored by the parental strain had been lost in all cases and none of them regained the ability to produce plantaricin OL15 suggesting that the production of plantaricin OL15 is plasmid linked. Plantaricin OL15 was not inactived by amylase and lipase suggesting that plantaricin OL15 activity was not dependent on the presence of either a carbohydrate or lipid moiety. Plantaricin OL15 showed activity against lactic acid bacteria Plasmid DNA studies in Lactobacillus plantarum strains isolated from olive fermentations: production of and immunity to plantaricin OL15 is associated to a 9.6 Kb plasmid (pOL15)

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Heterogeneity of Lactobacillus plantarum isolates from Feta cheese throughout ripening

Cited by 53 publications

References 18 publications

Characterization of Lactobacillus from Algerian goat's milk based on phenotypic, 16S rDNA sequencing and their technological properties

Characterization of Lactobacillus from Algerian goat's milk based on phenotypic, 16S rDNA sequencing and their technological properties

Gluten induces coeliac-like disease in sensitised mice involving IgA, CD71 and transglutaminase 2 interactions that are prevented by probiotics

Plasmid DNA studies in <i>Lactobacillus plantarum</i> strains isolated from olive fermentations: production of and immunity to plantaricin OL15 is associated to a 9.6 Kb plasmid (pOL15)

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