Heterogeneity in the presence of CD4-like molecules on human spermatozoa

Dimitrova-Dikanarova, Dimitrina; Marinova, T.-S. T.-S.; Fichorova, Raina N.

doi:10.1111/j.1439-0272.1998.tb01391.x

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…CD4 (cluster of differentiation 4) is a transmembrane glycoprotein found in several mammal cells including spermatozoa (Gobert et al ., 1990; Dimitrova-Dikanarova et al ., 1998). Lavitrano et al .…”

Section: Introductionmentioning

confidence: 99%

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Flow cytometric sex sorting affects CD4 membrane distribution and binding of exogenous DNA on bovine sperm cells

Domingues

Silveira

Komninou

et al. 2017

Zygote

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Bovine sex-sorted sperm have been commercialized and successfully used for the production of transgenic embryos of the desired sex through the sperm-mediated gene transfer (SMGT) technique. However, sex-sorted sperm show a reduced ability to internalize exogenous DNA. The interaction between sperm cells and the exogenous DNA has been reported in other species to be a CD4-like molecule-dependent process. The flow cytometry-based sex-sorting process subjects the spermatozoa to different stresses causing changes in the cell membrane. The aim of this study was to elucidate the relationship between the redistribution of CD4-like molecules and binding of exogenous DNA to sex-sorted bovine sperm. In the first set of experiments, the membrane phospholipid disorder and the redistribution of the CD4 were evaluated. The second set of experiments was conducted to investigate the effect of CD4 redistribution on the mechanism of binding of exogenous DNA to sperm cells and the efficiency of lipofection in sex-sorted bovine sperm. Sex-sorting procedure increased the membrane phospholipid disorder and induced the redistribution of CD4-like molecules. Both X-sorted and Y-sorted sperm had decreased DNA bound to membrane in comparison with the unsorted sperm; however, the binding of the exogenous DNA was significantly increased with the addition of liposomes. Moreover, we demonstrated that the number of sperm-bound exogenous DNA was decreased when these cells were preincubated with anti-bovine CD4 monoclonal antibody, supporting our hypothesis that CD4-like molecules indeed play a crucial role in the process of exogenous DNA/bovine sperm cells interaction.

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Section: Introductionmentioning

confidence: 99%

“…CD4 (cluster of differentiation 4) is a transmembrane glycoprotein found in several mammal cells including spermatozoa (Gobert et al, 1990;Dimitrova-Dikanarova et al, 1998). Lavitrano et al (1997) demonstrated that membrane CD4-like molecules are responsible for the interaction and binding of exogenous DNA in mice sperm.…”

Section: Introductionmentioning

confidence: 99%

Flow cytometric sex sorting affects CD4 membrane distribution and binding of exogenous DNA on bovine sperm cells

Domingues

Silveira

Komninou

et al. 2017

Zygote

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“…Several macromolecules on the sperm surface have been implicated as HIV‐1 binding ligands. These include proteins such as the mannose receptor and CD4 and CD4 like proteins . However, several lines of evidence argue against the presence of CD4 on the sperm surface .…”

Section: Introductionmentioning

confidence: 99%

Sperm can act as vectors for HIV‐1 transmission into vaginal and cervical epithelial cells

Young

Tatieng

Kongmanas

et al. 2019

American J Rep Immunol

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Problem Sperm are the major cells in semen. Human sperm possess a number of HIV‐1 gp120 binding ligands including sulfogalactosylglycerolipid (SGG). However, the mechanisms of how sperm capture HIV‐1 onto their surface are unclear. Furthermore, the ability of sperm to deliver HIV‐1 to vaginal/cervical epithelial cells lining the lower female reproductive tract, as a first step in HIV‐1 transmission, needs to be determined. Method of study Sperm from healthy donors were incubated with dual‐tropic HIV‐1CS204 (clinical isolate), and virus capture was determined by p24 antigen ELISA. The involvement of SGG in HIV‐1 capture was assessed by determining Kd values of HIV‐1 gp120‐SGG binding as well as computational docking of SGG to the gp120 V3 loop. The ability of sperm‐associated HIV‐1 to infect peripheral blood mononuclear cells (PBMCs) and TZM‐bl indicator cells was determined. Lastly, infection of vaginal (Vk2/E6E7), ectocervical (Ect1/E6E7), and endocervical (End1/E6E7) epithelial cells mediated by HIV‐1–associated sperm was evaluated. Results Sperm were able to capture HIV‐1 in a dose‐dependent manner, and the capture reached a maximum within 5 minutes. Captured HIV‐1, however, could be removed from sperm by Percoll‐gradient centrifugation. Affinity of gp120 for SGG was substantial, implicating sperm SGG in HIV‐1 capture. Sperm‐associated HIV‐1 could productively infect PBMCs and TZM‐bl cells, and was capable of being transmitted into vaginal/cervical epithelial cells. Conclusion Sperm are able to capture HIV‐1, which remains infectious and is able to be transmitted into vaginal/cervical epithelial cells, a result indicating the importance of sperm in HIV transmission.

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Viruses in the reproductive tract: On their way to the germ line?

Kuyl

Berkhout

2020

Virus Research

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Heterogeneity in the presence of CD4-like molecules on human spermatozoa

Cited by 5 publications

References 18 publications

Flow cytometric sex sorting affects CD4 membrane distribution and binding of exogenous DNA on bovine sperm cells

Flow cytometric sex sorting affects CD4 membrane distribution and binding of exogenous DNA on bovine sperm cells

Sperm can act as vectors for HIV‐1 transmission into vaginal and cervical epithelial cells

Viruses in the reproductive tract: On their way to the germ line?

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