Heterochromatin Heterogeneity Revealed by Restriction Endonuclease Digestion and Subsequent C-banding on Bovine Metaphase Chromosomes

Hidas, A.

doi:10.1111/j.1601-5223.1995.00285.x

Cited by 3 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same author tested two different REs (Hinf I and Taq I) and sequentially C-banding (with Giemsa staining) in cattle chromosomes, and reported that some CH blocks have been removed, as a consequence of the prior RE digestion; moreover, some CH blocks revealed minor subsets, and so forth the CH was heterogeneous. Hidas (1992) also concluded that this type of experimental approach is a useful tool for the study of CH heterogeneity and composition. Later in 1998, Fernandez-Garcıa et al, applied three REs (Alu I, Taq I, and Hae III) in fixed sheep chromosomes for the CH analysis (with Giemsa and fluorochromes); however, they did not use sequential C-banding.…”

Section: Discussionmentioning

confidence: 97%

“…The study of CH heterogeneity with in situ RE digestion and subsequent C-banding in Bovidae chromosomes, as far as we know, is only described in bovine chromosomes (Hidas, 1992). The same author tested two different REs (Hinf I and Taq I) and sequentially C-banding (with Giemsa staining) in cattle chromosomes, and reported that some CH blocks have been removed, as a consequence of the prior RE digestion; moreover, some CH blocks revealed minor subsets, and so forth the CH was heterogeneous.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Analysis (Hippotragini versus Caprini, Bovidae) of X-Chromosome's Constitutive Heterochromatin by in situ Restriction Endonuclease Digestion: X-Chromosome Constitutive Heterochromatin Evolution

2004

View full text Add to dashboard Cite

The Bovidae X-chromosome shows a considerable variation, in contrast to the preservative autosomal conservatism. The X-chromosome variation is mostly a consequence of the constitutive heterochromatin (CH) variation; in what respect to its amount and position. This is especially common among the non-Bovinae subfamilies and tribes. In order to characterize the X-chromosome CH in non-Bovinae species -Hippotragini and Caprini tribes -we have used restriction endonuclease digestion on fixed chromosomes and sequential C-banding. With these techniques we were able to distinguish between the two X-chromosome types (Hippotragini and Caprini) CH, in what respect to its position and molecular nature. Moreover, we define at least, six subclasses of CH in both X-chromosome types analyzed. Evolutionary considerations were draw based on the results obtained. The technology used here for the analysis of the Bovidae X-chromosome CH showed to be more evolutionary informative than the classical approaches.Abbreviations: BAC -bacterial artificial chromosomes; CBP-banding -C-bands by barium hydroxide using propidium iodide; CH -constitutive heterochromatin; Chr -chromosome; DAPI -4¢-6-diamidino-2-phenylindole; DNA -deoxyribonucleic acid; FISH -fluorescent in situ hybridization; GTD-banding -G-bands by trypsin with DAPI; MYBP -million years ago before present; NAA -number of autosomal arms; PBS -phosphate-buffered saline; RE-banding -restriction endonuclease banding; RE-bands -restriction endonuclease bands; RE -restriction endonuclease; REs -restriction endonucleases; SSC -saline sodium citrate.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Comparative Analysis (Hippotragini versus Caprini, Bovidae) of X-Chromosome's Constitutive Heterochromatin by in situ Restriction Endonuclease Digestion: X-Chromosome Constitutive Heterochromatin Evolution

2004

View full text Add to dashboard Cite

show abstract

“…Chromosome digestion with restriction endonucleases (RE) has been used to induce banding in many different animal species. In cattle, restriction enzymes were used mainly to characterise the structure and variability of centromeric heterochromatin (H idas 1995; C haves et al 2000). Here, we report the results of the digestion of cattle chromosomes with two endonucleases: Msp I and Hae III.…”

mentioning

confidence: 99%

Standardization of MspI and HaeIII restriction karyotypes in cattle

Cymbron¹,

Chaves

Adega

et al. 2004

Hereditas

View full text Add to dashboard Cite

show abstract

Untitled

Chaves

Heslop-Harrison

Guedes-Pinto

2000

Chromosome Research

View full text Add to dashboard Cite

The centromeric regions and alpha-satellite I sequence were studied on chromosomes 1, 29 and the rob(1;29) translocation in a Portuguese breed of cattle, Barrosa, carrying the translocation. Rob(1;29) centromeric regions showed heterochromatic bands with propidium iodide but, unlike the acrocentric autosomes, no strong centromeric bands were revealed with chromomycin A3. An alpha-satellite I sequence was not found at the centromeres of the X, Y and rob(1;29) chromosomes in the breed, although it was present at the centromeres of all acrocentric chromosomes including 1 and 29. Restriction enzyme banding with MspI revealed polymorphisms between different rob(1;29) chromosomes in both centromeric and intercalary regions. The data show that the centromeric region of the rob(1;29) chromosome has lost the alpha-satellite I sequences, while retaining other heterochromatin, and suggest that this important and widespread translocation has occurred multiple times.

show abstract

Heterochromatin Heterogeneity Revealed by Restriction Endonuclease Digestion and Subsequent C-banding on Bovine Metaphase Chromosomes

Cited by 3 publications

References 7 publications

Comparative Analysis (Hippotragini versus Caprini, Bovidae) of X-Chromosome's Constitutive Heterochromatin by in situ Restriction Endonuclease Digestion: X-Chromosome Constitutive Heterochromatin Evolution

Comparative Analysis (Hippotragini versus Caprini, Bovidae) of X-Chromosome's Constitutive Heterochromatin by in situ Restriction Endonuclease Digestion: X-Chromosome Constitutive Heterochromatin Evolution

Standardization of MspI and HaeIII restriction karyotypes in cattle

Untitled

Contact Info

Product

Resources

About