2013
DOI: 10.18632/aging.100559 View full text |Buy / Rent full text
|
|

Abstract: This work builds upon our findings that proteins secreted by hESCs exhibit pro-regenerative activity, and determines that hESC-conditioned medium robustly enhances the proliferation of both muscle and neural progenitor cells. Importantly, this work establishes that it is the proteins that bind heparin which are responsible for the pro-myogenic effects of hESC-conditioned medium, and indicates that this strategy is suitable for enriching the potentially therapeutic factors. Additionally, this work shows that hE… Show more

Help me understand this report

Search citation statements

Order By: Relevance
Select...
3
2
4
26
0

Year Published

2014
2014
2014
2014

Publication Types

Select...
1

Relationship

1
0

Authors

Journals

4
26
0
Order By: Relevance
“…Proliferation was significantly enhanced and differentiation inhibited in both human and mouse muscle progenitor cells by the hESC-conditioned medium (Figure 1A, B). Analogously, hESC growth medium (mTeSR1) conditioned by hESC culture enhanced myoblast proliferation more effectively than mTeSR1 alone, though mouse myoblasts exhibited significantly enhanced proliferation in both media (Supplementary Figure 1A, B and [17]).…”
Section: Resultsmentioning
“…We recently published that in the mouse system, the pro-myogenic activity of hESC-conditioned Opti-MEM is contained in proteins with heparin-binding activity [17]. To explore the evolutionary conservation of this phenomenon in a human system, we cultured primary human muscle progenitor cells in a 50/50 mixture of the low-mitogen differentiation medium plus hESC-conditioned Opti-MEM that was either complete or depleted of heparin-binding proteins.…”
Section: Resultsmentioning
See 3 more Smart Citations