Herpes gestationis. New immunologic findings

Bushkell, Lawrence L.

doi:10.1001/archderm.110.1.65

Cited by 27 publications

(18 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A total of nine skin biopsies was studied from our seven patients. In only one of these skin lesions (the second skin biopsy from case 1) was BMZ deposition of IgG noted, findings reported previously (2). No immunoglobulins (IgG, IgM, IgA or IgE) were noted to be bound to the BMZ in any of the other skin lesions tested.…”

Section: Methodssupporting

confidence: 66%

“…Cord serum samples from two of our patients were also tested by the various indirect IF and This case was reported previously (2). § Not tested.…”

Section: Methodsmentioning

confidence: 85%

“…Other herpes gestationis patients who demonstrate IgG deposition in skin lesions but who lack circulating BMZ antibodies, like some bullous pemphigoid patients, have also recently been described (3,4). Using various IF techniques, we wish to report our observations in six cases of herpes gestationis in addition to our previously reported case (2). IF studies of cord serum samples from two of our patients and our attempts to further characterize the so-called HG factor as IgG, are also presented.…”

Section: Introductionmentioning

confidence: 98%

“…Using immunofluorescence (IF)1 techniques, Provost (2). Other herpes gestationis patients who demonstrate IgG deposition in skin lesions but who lack circulating BMZ antibodies, like some bullous pemphigoid patients, have also recently been described (3,4).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The immunopathology of herpes gestationis. Immunofluorescence studies and characterization of "HG factor".

Jordon¹,

Heine²,

Tappeiner³

et al. 1976

J. Clin. Invest.

139

View full text Add to dashboard Cite

A B S T R A C T Nine skin biopsies from seven herpes gestationis patients were studied by immunofluorescence (IF) techniques. Basement membrane zone (BMZ) deposition of C3 and properdin was present in all nine skin specimens, while IgG deposition was apparent in only one. With in vitro C3 IF staining, positive BMZ staining (HG factor activity) was noted with all seven of our patients' serum samples tested. By standard indirect IF staining, however, only one of these serum samples contained BMZ antibodies of the IgG type. Two cord serum samples, tested by these same methods, yielded positive in vitro C3 staining (HG factor activity) but negative indirect IF staining (IgG). HG factor activity was found to be stable at 56°C for 30 min and in two of three specimens at 56°C for 1 h. Treatment of the complement source (normal human serum) used in the in vitro C3 staining assay with Mg2-EGTA or use of C2-deficient serum as the complement source inhibited HG factor activity. HG factor blocked the specific staining of the BMZ of normal human skin by labeled bullous pemphigoid antibodies. By sucrose density gradient ultracentrifugation and gel chromatography (Sephadex G-200), HG factor activity eluted with IgGcontaining fractions. The highly purified IgG fraction of two herpes gestationis sera was also positive for HG factor activity. Our studies suggest that HG factor is an IgG antibody that may not be demonstrable by conventional IF methods, but which activates the classical complement pathway.

show abstract

Section: Methodssupporting

confidence: 66%

“…Cord serum samples from two of our patients were also tested by the various indirect IF and This case was reported previously (2). § Not tested.…”

Section: Methodsmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The immunopathology of herpes gestationis. Immunofluorescence studies and characterization of "HG factor".

Jordon¹,

Heine²,

Tappeiner³

et al. 1976

J. Clin. Invest.

139

View full text Add to dashboard Cite

show abstract

“…It had been considered a variant of erythema multiforme or dermatitis herpetiformis until Provost and Tomasi demonstrated HG to be a distinct disease (1). They studied two patients with HG and found, on direct immunofluorescence Receized for puiblication 5 November 1975 and in revised form 3 February 1976. 1 Abbreviations used in this paper: BM, basement membrane; BP, bullous pemphigoid; EGTA, ethyleneglycoltetracetic acid; factor B, properdin factor B; FITC, fluorescein isothiocyanate; HG, herpes gestationis; IF, immunofluorescence; NFS, normal fresh serum; P, properdin; PBS, phosphate-buffered saline.…”

Section: Introductionmentioning

confidence: 99%

Herpes gestationis. Immunopathology and characterization of the HG factor.

Katz¹,

Hertz²,

Yaoita³

1976

J. Clin. Invest.

174

View full text Add to dashboard Cite

A B S T R A C T Five patients with herpes gestationis, a blistering disease of pregnancy, were studied immunologically. All had in vivo deposition of C3 in a linear band along the basement membrane zone of lesional and normnal-appearing skin, the location of early blister formation. Immunoglobulin deposition was more variable, though four patients had evidence of in vivo bound IgG at the same site. A circulating, complement binding herpes gestationis factor was demonstrated in the sera of four of the patients, its concentration unrelated to the activity of clinical disease. Characterization of this factor by sucrose gradient ultracentrifugation, specific absorption studies, and papain digestion indicates that it is an IgG. Evidence exists for involvement of both the classical and alternate complement pathways in vivo, though in vitro studies implicate the classical pathway as the primary route of complement activation. Three offspring were studied, none with clinical involvement; one slhowed in vivo deposition of C3 at the basement membrane zone of normal skin and a second showed the herpes gestationis factor in cord blood.

show abstract