Hepatoprotective Potential of Pomegranate in Curbing the Incidence of Acute Liver Injury by Alleviating Oxidative Stress and Inflammatory Response

Ali, Hamid; Jahan, Azra; Samrana, Samrana; Ali, Abid; Ali, Safdar; Kabir, Nurul; Ali, Amjad; Ullah, Riaz; Mothana, Ramzi A.; Murtaza, Bibi Nazia; Kalim, Muhammad

doi:10.3389/fphar.2021.694607

Cited by 17 publications

(6 citation statements)

References 45 publications

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“…However, no significant increase was recorded when PPE was administered to the CCl 4 -treated group. This result was in disagreement with the study of Ghamry [30] and Ali et al [31] where PPE significantly elevated the GSH level of the CCl 4 -treated group. The non-significant increase recorded in our study might likely be due to the short treatment duration.…”

Section: Impact Of CCL 4 Administration On Oval Cell Regenerationcontrasting

confidence: 99%

The Role of Pomegranate Peel Extract in Improving Hepatotoxicity, and hMSH2 Expression in CCI 4 -Treated Rats

Domiaty¹

2022

Int J Pharm Res Allied Sci

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Recently, pomegranate fruit and its leaves have found wide usage as a natural treatment for several ailments. In this study, we investigated the restorative potentials of pomegranate (Punica granatum) peel extract against hepatic damage brought on by calcium tetrachloride (CCl 4 ) in rats and its effects on the human mutS homolog 2 (hMSH2) gene expression. Four groups of twenty male Wistar rats (n =5) were created at random. Group I was the untreated control group. Group II was given a dose of 0.4 ml CCl 4 intraperitoneally (IP) for two consecutive days/week for 3 weeks. Group III was given a daily dose (500 mg/ kg b.w) of pomegranate peel extract (PPE) for 3 weeks. Group IV received an IP of CCl 4 for two consecutive days/week for 3weeks followed by a daily oral dose of (500 mg/ kg b.w) of PPE for 3 weeks. Bodyweight, relative liver weight, serum AST, ALT, bilirubin, tissue GSH, and MDA, the expression of the hMSH2 gene, liver histology, and immunohistochemistry were assessed. Our results showed that the administration of CCl 4 to rats did not affect their body weight and relative liver weight. However, CCl 4 administration resulted in a decrease in tissue GSH, an increase in serum AST, ALT, bilirubin, lipid peroxidation, modification of liver histology, and immunohistochemistry, and the downregulation of the expression of the hMSH2 gene. Intriguingly, PPE treatment following CCl 4 administration to rats attenuated these changes. Taken together, our study reveals the potential of PPE for its use in the treatment of liver damage.

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Section: Impact Of CCL 4 Administration On Oval Cell Regenerationcontrasting

confidence: 99%

The Role of Pomegranate Peel Extract in Improving Hepatotoxicity, and hMSH2 Expression in CCI 4 -Treated Rats

Domiaty¹

2022

Int J Pharm Res Allied Sci

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“…ALT, AST, and ALP are valuable biochemical indicators for examining early hepatotoxicity (Ali et al, 2021). As seen in Figure 3, relative to the control group, in the model group, the ALT, AST, and ALP activities were dramatically increased (p < 0.001, p < 0.01).…”

Section: Effects Of Hydroxysafflor Yellow a On Biochemical Indicatorsmentioning

confidence: 97%

Mechanism of hydroxysafflor yellow A on acute liver injury based on transcriptomics

Hou

Zhang

et al. 2022

Front. Pharmacol.

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Objective: To investigate how Hydroxysafflor yellow A (HSYA) effects acute liver injury (ALI) and what transcriptional regulatory mechanisms it may employ.Methods: Rats were randomly divided into five groups (n = 10): Control, Model, HSYA-L, HSYA-M, and HSYA-H. In the control and model groups, rats were intraperitoneally injected with equivalent normal saline, while in the HSYA groups, they were also injected with different amounts of HSYA (10, 20, and 40 mg/kg/day) once daily for eight consecutive days. One hour following the last injection, the control group was injected into the abdominal cavity with 0.1 ml/100 g of peanut oil, and the other four groups got the same amount of a peanut oil solution containing 50% CCl4. Liver indexes were detected in rats after dissection, and hematoxylin and eosin (HE) dyeing was utilized to determine HSYA’s impact on the liver of model rats. In addition, with RNA-Sequencing (RNA-Seq) technology and quantitative real-time PCR (qRT-PCR), differentially expressed genes (DEGs) were discovered and validated. Furthermore, we detected the contents of anti-superoxide anion (anti-O2−) and hydrogen peroxide (H2O2), and verified three inflammatory genes (Icam1, Bcl2a1, and Ptgs2) in the NF-kB pathway by qRT-PCR.Results: Relative to the control and HSYA groups, in the model group, we found 1111 DEGs that were up-/down-regulated, six of these genes were verified by qRT-PCR, including Tymp, Fabp7, Serpina3c, Gpnmb, Il1r1, and Creld2, indicated that these genes were obviously involved in the regulation of HSYA in ALI model. Membrane rafts, membrane microdomains, inflammatory response, regulation of cytokine production, monooxygenase activity, and iron ion binding were significantly enriched in GO analysis. KEGG analysis revealed that DEGs were primarily enriched for PPAR, retinol metabolism, NF-kB signaling pathways, etc. Last but not least, compared with the control group, the anti-O2− content was substantially decreased, the H2O2 content and inflammatory genes (Icam1, Bcl2a1, and Ptgs2) levels were considerably elevated in the model group. Compared with the model group, the anti-O2− content was substantially increased, the H2O2 content and inflammatory genes (Icam1, Bcl2a1, and Ptgs2) levels were substantially decreased in the HSYA group (p < 0.05).Conclusion: HSYA could improve liver function, inhibit oxidative stress and inflammation, and improve the degree of liver tissue damage. The RNA-Seq results further verified that HSYA has the typical characteristics of numerous targets and multiple pathway. Protecting the liver from damage by regulating the expression of Tymp, Fabp7, Serpina3c, Gpnmb, Il1r1, Creld2, and the PPAR, retinol metabolism, NF-kappa B signaling pathways.

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“…Used in DM and cardiovascular disease. [ 188 ] Rhinacanth-us nasuta (Acanthace-ae) Methanolic extract of Leaves Male Wistar rats Streptozoto-cin (STZ) 200 mg/kg/day p.o. ALT↓,AST↓ Used in eczema, herpes, pulmonary tuberculosis, hepatitis, diabetes, hypertension and skin Diseases.…”

Section: Hepatoprotective Potentials Of Medicinal Plants and Their Bi...mentioning

confidence: 99%

Medicinal herbs and their metabolites with biological potential to protect and combat liver toxicity and its disorders: A review

Islam Shawon,

Nargis Reyda,

Qais

2024

Heliyon

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Hepatoprotective Potential of Pomegranate in Curbing the Incidence of Acute Liver Injury by Alleviating Oxidative Stress and Inflammatory Response

Cited by 17 publications

References 45 publications

The Role of Pomegranate Peel Extract in Improving Hepatotoxicity, and hMSH2 Expression in CCI 4 -Treated Rats

The Role of Pomegranate Peel Extract in Improving Hepatotoxicity, and hMSH2 Expression in CCI 4 -Treated Rats

Mechanism of hydroxysafflor yellow A on acute liver injury based on transcriptomics

Medicinal herbs and their metabolites with biological potential to protect and combat liver toxicity and its disorders: A review

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