Hepatoprotective Effect of Prolactin and Cysteine Contained in Perfusion and Preservation Solutions on Porcine Liver Stored in Simple Hypothermia

Pomeranian Journal of Life Sciences

Ryszka

2020

Introduction: Biolasol is a solution developed in Poland for flushing the kidneys, liver, heart and pancreas by simple hypothermia method prior to transplantation. The solution supports the cellular integrity of grafts during ischemia-reperfusion injury (IRI).The aim of the study was to evaluate the impact of the concentration of prolactin added to Biolasol on selected biochemical parameters of kidney injury.Materials and methods: Biolasol was modified by the addition of prolactin at 1 μg/L, 10 μg/L and 100 μg/L and by ascorbicacid at 0.5 mmol/L. After 2 h and 48 h of storage, the levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase enzymes, sodium and potassium concentrations, pH and osmolarity parameters were assessed in the perfusates.Results: The addition of prolactin to Biolasol significantly improves the biochemical parameters of grafts in the models of rinsing, perfusion and reperfusion of isolated porcine kidneys.Conclusions: The study indicates the nephroprotective role of Biolasol with the addition of vitamin C and prolactin at a 100 μg/L.

Section: Discussionmentioning

confidence: 99%

The effect of prolactin concentration on the efficacy of storing isolated porcine kidneys in a modified Biolasol solution

Pomeranian Journal of Life Sciences

Ryszka

2020

“…A number of our studies indicate the hepatoprotective and nephroprotective effects of prolactin [ 8 , 20 , 21 ]. PRL protects the structure and function of cells against the negative effects of ischemia and hypoxia [ 20 , 37 ]. The addition of this hormone to preservation solutions affects the regeneration of cells after hepatectomy and nephrectomy.…”

Section: Discussionmentioning

confidence: 99%

“…The addition of this hormone to preservation solutions affects the regeneration of cells after hepatectomy and nephrectomy. It also ensures the integrity of the cell membrane and contributes to the maintenance of normal balance of ions and normal morphological parameters of the liver and kidneys [ 20 , 37 ]. The addition of 1 μ g/l rh-PRL to Biolasol solution reduces ALT and AST activity during reperfusion [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Modified Biolasol Solution on the Efficacy of Storing Isolated Porcine Kidneys

BioMed Research International

Ryszka

2018

Biolasol is a newly developed solution for storing the liver, pancreas, kidneys, and heart by simple hypothermia. It exhibits high efficacy in maintaining structural and functional integrity of the graft prior to its transplantation. The solution was modified by the addition of ascorbic acid (0.088g/l) and ascorbic acid with prolactin (1 μg/l PRL + 0.088g/l vitamin C). The effectiveness of the obtained solutions in the protection of nephrons of isolated porcine kidneys was assessed based on the analysis of the activity of ALT (alanine aminotransferase), AST (aspartate aminotransferase), and LDH (lactate dehydrogenase) as well as lactate concentration determined in perfundates collected after 2 h (0′ and 30′ preservation) and 48 h (0′ and 30′ preservation) of graft storage. It has been found that the synergistic action of Biolasol components determines the integrity and stability of cell membranes, which in turn affects the proper functioning of the organ after transplantation. The addition of ascorbic acid and prolactin to Biolasol affects the maintenance of the normal cytoskeleton of the stored graft.

“…The combination of prolactin with cysteine (an antioxidant) in HTK reduced the amount of released transaminases (AST and ALT), lactate dehydrogenase (LDH), and lactic acid. Modification of the solution also resulted in the smaller release of K + and Mg 2+ ions and larger release of Na + and Ca 2+ ions [6]. Szulc-Musioł et al analysed the pharmacological effectiveness of prolactin in the model of rabbit liver ischemia based on Pringle's manoeuvre.…”

Section: Hormonal Additivesmentioning

confidence: 99%

Pharmacological Benefits and Risk of Using Hormones in Organ Perfusion and Preservation Solutions in the Aspect of Minimizing Hepatic Ischemia-Reperfusion Injury during Storage

BioMed Research International

2019

For several years, research has been carried out on the effectiveness of solutions for perfusion and preservation of organs, including the liver. There is a search for an optimal pharmacological composition of these solutions, allowing to preserve or improve vital functions of the organ for as long as possible until it is transplanted into a recipient. Hormones due to their properties, often resulting from their pleiotropic effects, may be a valuable component for optimizing the composition of liver perfusion and preservation solutions. The paper presents the current state of knowledge on liver perfusion and preservation solutions modified with hormones. It also shows the characteristics of the hormones evaluated, taking into account their physiological functions in the body.