Hepatocyte growth factor (HGF) signals through SHP2 to regulate primary mouse myoblast proliferation

Li, J; Reed, Sarah; Johnson, Sally

doi:10.1016/j.yexcr.2009.04.011

Cited by 35 publications

(28 citation statements)

References 50 publications

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“…R1b and/or R2b) to elicit its response (ED 50 Z0.1-1 ng/ml; Ornitz 2000, Powers et al 2000). Similar biphasic dose responses to FGF supplementation, and other paracrineacting factors, for that matter, are evident in various cell types (Butterwith et al 1993, Murono et al 1993, Halevy et al 1994, Garcia-Maya et al 2006, Li et al 2009a). This phenomenon could have been caused by receptor downregulation events associated with ligand overloading (Murono et al 1993).…”

Section: Discussionmentioning

confidence: 90%

Fibroblast growth factor 10 enhances bovine oocyte maturation and developmental competence in vitro

Zhang¹,

Hansen²,

Ealy³

2010

Reproduction

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The ability of oocytes to resume meiosis, become fertilized, and generate viable pregnancies is controlled during folliculogenesis by several endocrine and paracrine factors. The aim of this work is to determine whether fibroblast growth factor 10 (FGF10) is an oocyte competent factor. Transcripts for each of the four FGF receptor types (FGFR) were present in cumulus and oocytes after their extraction from the follicles. FGFR1 transcripts predominated in cumulus cells whereas FGFR2 was most abundant in oocytes. Exposing the cumulus-oocyte complexes to FGF10 during in vitro maturation did not affect cleavage rates, but increases (P!0.05) in the percentage of embryos at the 8-16-cell stage on day 3 and at the blastocyst stage on day 7, which were evident in FGF10-supplemented oocytes. The progression of oocytes through meiosis and cumulus expansion was increased (P!0.05) by FGF10. The importance of the endogenous sources of FGFs was examined by adding anti-FGF10 IgG during oocyte maturation. Blocking endogenous FGF10 activity decreased (P!0.05) the percentage of oocytes developing into blastocysts and limited (P!0.05) cumulus expansion. Expression profiles of putative cumulus and oocyte competency markers were examined for their involvement in FGF10-mediated responses. FGF10 influenced the expression of CTSB and SPRY2 in cumulus cells and BMP15 in oocytes. In summary, this work provides new insight into the importance of FGFRs and locally derived FGF10 during oocyte maturation in cattle. Its subsequent impact on in vitro embryo development implicates it as a noteworthy oocyte competent factor.

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Section: Discussionmentioning

confidence: 90%

Fibroblast growth factor 10 enhances bovine oocyte maturation and developmental competence in vitro

Zhang¹,

Hansen²,

Ealy³

2010

Reproduction

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“…At necropsy, muscle samples were collected from the left semitendinosus muscle (STN) from a subset of animals for satellite cell isolation (CON: birth, n = 2; 3 months, n = 3; RES: birth, n = 3; 3 months, n = 2). Satellite cells were isolated using a standard enzymatic digestion and differential centrifugation protocol and frozen until further use (Li et al, 2009). In our hands, >95% of satellite cells isolated from sheep by this method are desmin positive, demonstrating purity of the population.…”

Section: Methodsmentioning

confidence: 99%

Restricted maternal nutrition alters myogenic regulatory factor expression in satellite cells of ovine offspring

Raja

Hoffman

Govoni

et al. 2016

Animal

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Poor maternal nutrition inhibits muscle development and postnatal muscle growth. Satellite cells are myogenic precursor cells that contribute to postnatal muscle growth, and their activity can be evaluated by the expression of several transcription factors. Pairedbox (Pax)7 is expressed in quiescent and active satellite cells. MyoD is expressed in activated and proliferating satellite cells and myogenin is expressed in terminally differentiating cells. Disruption in the expression pattern or timing of expression of myogenic regulatory factors negatively affects muscle development and growth. We hypothesized that poor maternal nutrition during gestation would alter the in vitro temporal expression of MyoD and myogenin in satellite cells from offspring at birth and 3 months of age. Ewes were fed 100% or 60% of NRC requirements from day 31 ± 1.3 of gestation. Lambs from control-fed (CON) or restricted-fed (RES) ewes were euthanized within 24 h of birth (birth; n = 5) or were fed a control diet until 3 months of age ( n = 5). Satellite cells isolated from the semitendinosus muscle were used for gene expression analysis or cultured for 24, 48 or 72 h and immunostained for Pax7, MyoD or myogenin. Fusion index was calculated from a subset of cells allowed to differentiate. Compared with CON, temporal expression of MyoD and myogenin was altered in cultured satellite cells isolated from RES lambs at birth. The percent of cells expressing MyoD was greater in RES than CON ( P = 0.03) after 24 h in culture. After 48 h of culture, there was a greater percent of cells expressing myogenin in RES compared with CON ( P < 0.001). After 72 h of culture the percent of satellite cells expressing myogenin in RES was less than CON ( P < 0.01). There were no differences in the gene expression of Pax7, Myf5 or MyoD in isolated satellite cells at birth ( P > 0.05). In satellite cells from RES lambs at 3 months of age, the percent of cells expressing MyoD and myogenin were greater than CON after 72 h in culture ( P < 0.05). Fusion index was reduced in RES lambs at 3 months of age compared with CON ( P < 0.001). Restricted nutrition during gestation alters the temporal expression of myogenic regulatory factors in satellite cells of the offspring, which may reduce the pool of myoblasts, decrease myoblast fusion and contribute to the poor postnatal muscle growth previously observed in these animals.

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“…It also stimulates the proliferation and chondrogenic [11], adipogenic and myogenic differentiation of mesenchymal stem cells, also promotes neuronal differentiation [12] and induces a chemotactic effect on vascular endothelial cells. In addition, it has also been demonstrated that hepatocyte growth factor (HGF), mainly present in the plasma, stimulates cell proliferation [13] and migration while inhibits NF-B, a key nuclear factor implicated in inflammatory responses [14]. It is also remarkable the influence of HGF as an antifibrotic mediator [15,16].…”

Section: Letter To the Editormentioning

confidence: 99%

The P makes the difference in plasma rich in growth factors (PRGF) technology

Anitua¹,

Sánchez²,

Prado³

et al. 2011

Platelets

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Hepatocyte growth factor (HGF) signals through SHP2 to regulate primary mouse myoblast proliferation

Cited by 35 publications

References 50 publications

Fibroblast growth factor 10 enhances bovine oocyte maturation and developmental competence in vitro

Fibroblast growth factor 10 enhances bovine oocyte maturation and developmental competence in vitro

Restricted maternal nutrition alters myogenic regulatory factor expression in satellite cells of ovine offspring

The P makes the difference in plasma rich in growth factors (PRGF) technology

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