Hepatitis C virus-specific cellular and humoral immune responses following immunization with a multi-epitope fusion protein

Qiu, Feng; Bi, Shengli; Wang, Yue; Guo, Min-Zhuo; Yao, Yi; Chen, Si-yong; Guo, Yu; Shen, Liping; Jia, Zhiyuan

doi:10.3892/ijmm.2011.801

Cited by 4 publications

(3 citation statements)

References 20 publications

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“…Alternatively, differences in virus-host cell interactions produced by the E1B55K deletion might have stimulated Ad- and HIV-specific innate and adaptive immune responses that compensated for the increased levels of immunogen promoted by the wild-type virus. Indeed, differences in peptide and protein immunogen concentrations as little as 3- to 5-fold have led to detectable changes in immune responses [ 26 , 27 ]. For that reason, we expected the E1B55K wild-type vector to produce measurably higher immune responses in the immunized mice.…”

Section: Discussionmentioning

confidence: 99%

Beyond Oncolytics: E1B55K-Deleted Adenovirus as a Vaccine Delivery Vector

et al. 2016

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Type 5 human adenoviruses (Ad5) deleted of genes encoding the early region 1B 55-kDa (E1B55K) protein including Onyx-015 (dl1520) and H101 are best known for their oncolytic potential. As a vaccine vector the E1B55K deletion may allow for the insertion of a transgene nearly 1,000 base pairs larger than now possible. This has the potential of extending the application for which the vectors are clinically known. However, the immune priming ability of E1B55K-deleted vectors is unknown, undermining our ability to gauge their usefulness in vaccine applications. For this reason, we created an E1B55K-deleted Ad5 vector expressing full-length single chain HIVBaLgp120 attached to a flexible linker and the first two domains of rhesus CD4 (rhFLSC) in exchange for the E3 region. In cell-based experiments the E1B55K-deleted vector promoted higher levels of innate immune signals including chemokines, cytokines, and the NKG2D ligands MIC A/B compared to an E1B55K wild-type vector expressing the same immunogen. Based on these results we evaluated the immune priming ability of the E1B55K-deleted vector in mice. The E1B55K-deleted vector promoted similar levels of Ad5-, HIVgp120, and rhFLSC-specific cellular and humoral immune responses as the E1B55K wild-type vector. In pre-clinical HIV-vaccine studies the wild-type vector has been employed as part of a very effective prime-boost strategy. This study demonstrates that E1B55K-deleted adenoviruses may serve as effective vaccine delivery vectors.

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Section: Discussionmentioning

confidence: 99%

Beyond Oncolytics: E1B55K-Deleted Adenovirus as a Vaccine Delivery Vector

et al. 2016

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“…Additional strategies include molecules that induce innate immune responses, with secondary effects on adaptive responses (such as TLR‐9 ligands) that are either encoded within a vaccine construct or used as a vaccine adjuvant. Qiu et al (12) prepared a fusion protein in the vector of pET‐11d that included 3 conserved broadly neutralising B‐cell epitopes and a series of T‐cell epitopes located in the HCV NS3 region. In vivo, administration of this fusion construct resulted in specific CD8+ cytotoxic lymphocytes that recognised specific antigen sites that were detected by fluorescence activated cell sorter.…”

Section: Plans To Achieve the Goalsmentioning

confidence: 99%

Hepatitis B and C

Mohan

Karnsakul

Wirth

et al. 2012

J. pediatr. gastroenterol. nutr.

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A cute gastroenteritis (AGE) is an extremely frequent problem in childhood with 2 distinct consequences in rich and poor countries: hospital admissions and costs in the former, and death in the latter. AGE is the second leading cause of death in childhood, pneumonia being the first (1). The most frequent and dangerous agent is rotavirus, an infection with a typical target age distribution between 6 and 24 months of age and no specific geographical distribution.The severity of AGE in poor countries results from a number of factors, primarily poverty and lack of education, and also limited access to health care requiring sick children and their mothers often to travel great distances to reach a physician.A number of worldwide initiatives have been launched to reduce poverty and improve education and security. The Millenium Development Goals (MDG), a joint venture by the United Nations and the International Monetary Fund, the World Bank, and other organizations/agencies, are a series of 8 aims that have been established to reduce poverty and improve education, shelter, and security. The MDG number 4 (MDG4) is to reduce mortality in children younger than 5 years by two-thirds, between 1990 and 2015. One of the major causes of mortality in this age group is diarrhea. The MDG report in 2011 describes the success that has been obtained particularly in northern Africa and eastern Asia (2).

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“…Potent new direct acting antivirals (DAAs) have dramatically improved the success rates in disruption of the viral replication; however, high costs, side effects, and treatment failures still remain the main issues, underscoring the importance of developing therapeutic/prophylactic vaccines [25]. Several studies have shown that NS3 protein is a promising antigen for the purpose of anti-HCV vaccine development [26,27]. However, subunit vaccines induce a lower immune response compared to whole cell vaccines, requiring improved vaccine carriers/adjuvants [28].…”

Section: Discussionmentioning

confidence: 99%

Synthesis and characterization of physicochemical and immunological properties of recombinant NS3-G2 dendrimer conjugate

Javadi

Rahimi

Modaressi

et al. 2015

vacres

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Introduction: An effective vaccine against HCV infection is not available. The non-structural protein 3 (NS3) of the virus as an important immunogenic candidate has been utilized in various modules. Nanostructured polymers have been recently used for efficient vaccine and drug delivery. The aim of the current study was the synthesis of rNS3-G2 conjugate and preliminary evaluation of its immunogenicity. Methods: The dendrimer was synthesized and conjugated with purified recombinant NS3 (rNS3) protein. The physicochemical properties of the conjugate were evaluated by Zeta potential, FT-IR spectra and confirmed by atomic force microscopy (AFM). Immunogenicity of the conjugate was assessed in BALB/c mice. Results: Synthesis and conjugation of dendrimer G2 with the protein were confirmed and immunological assays showed that the conjugated form of the antigen induced higher titer of IgG compared to rNS3 antigen alone. Conclusion: The results showed that the antigenic structure of rNS3 was maintained when conjugated with the biodegradable and biocompatible G2 dendrimers and the immunogenic properties of the antigen were enhanced. Therefore the new formulation may have potential as a vaccine candidate.

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Hepatitis C virus-specific cellular and humoral immune responses following immunization with a multi-epitope fusion protein

Cited by 4 publications

References 20 publications

Beyond Oncolytics: E1B55K-Deleted Adenovirus as a Vaccine Delivery Vector

Beyond Oncolytics: E1B55K-Deleted Adenovirus as a Vaccine Delivery Vector

Hepatitis B and C

Synthesis and characterization of physicochemical and immunological properties of recombinant NS3-G2 dendrimer conjugate

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