Hepatitis C virus (HCV) genotypes in Spanish patients with HCV infection: relationship between HCV genotype 1b, cirrhosis and hepatocellular carcinoma

López‐Labrador, F. Xavier; Ampurdanès, Sergi; Forns, Xavier; Castells, Antoni; Saiz, Juan-Carlos; Costa, Josep; Bruix, Jordi; Tapias, José M. Sánchez; Anta, M. T. Jiménez de; Rodés, Juan

doi:10.1016/s0168-8278(97)80137-7

Cited by 108 publications

(85 citation statements)

References 32 publications

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“…HCV genotype was determined by restriction fragment length polymorphism after amplification of the 5Ј noncoding region of the HCV genome, as described previously. 20 If HCV RNA was undetectable, HCV genotype was determined with a serotyping assay that detects type-specific antibodies directed to epitopes encoded by the NS4 region of the genome (Murex HCV Serotyping; Abbot Cientifica S.A., Madrid, Spain). The assay identifies the HCV type (1-6) with high accuracy but does not provide information on the HCV subtype.…”

Section: Methodsmentioning

confidence: 99%

Nosocomial transmission of HCV in the liver unit of a tertiary care center

et al. 2004

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Despite its medical and legal implications, there are no prospective studies analyzing the incidence and mechanisms involved in the nosocomial transmission of hepatitis C virus (HCV) in liver units. This study prospectively investigates the nosocomial transmission of HCV in the liver unit of a tertiary care center from August 2000 to October 2002. The median prevalence of HCV infection among hospitalized patients was 50%. Anti-HCVnegative patients admitted to the liver unit during the study period were prospectively followed, and serum markers of HCV infection were repeated 6 months after discharge.

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Section: Methodsmentioning

confidence: 99%

Nosocomial transmission of HCV in the liver unit of a tertiary care center

et al. 2004

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“…HCV genotype was determined by restriction fragment length polymorphism (RFLP) analysis of the 5ЈNCR as previously described. 23,24 HCV-RNA concentration in serum was measured by the COBAS Amplicor HCV Monitor test, v2.0 (Roche Diagnostic Systems, Inc., Branchburg, NJ).…”

Section: Methodsmentioning

confidence: 99%

High amino acid variability within the NS5A of hepatitis C virus (HCV) is associated with hepatocellular carcinoma in patients with HCV-1b–related cirrhosis

et al. 2001

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“…HCV genotyping was performed by restriction fragment length polymorphism (RFLP) analysis of the 5Ј'NCR, basically as described. 25,27 Amplification of the NS3 Region and DNA Sequencing A segment of the NS3 region encompassing nucleotides 4429 to 4719 (positions referred to the HCV-1 prototype), which includes HLA-A2-restricted (KLVALGINAV) and HLA-A3-restricted (LIFCHSKKK) T-cell epitopes, was amplified by nested PCR. Several studies indicate that HCV Class I restricted T-cell epitopes located within the NS3 protein are commonly recognized by liver-infiltrating and circulating cytotoxic T lymphocytes (CTL) of HCV-infected patients.…”

Section: Hcv-rna Viral Load and Genotypingmentioning

confidence: 99%

Genetic variability of hepatitis C virus NS3 protein in human leukocyte antigen-A2 liver transplant recipients with recurrent hepatitis C

et al. 2004

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The association between the severity of chronic hepatitis C and the variability of the hepatitis C virus (HCV) genome remains controversial, but to our knowledge few data are available to date regarding T-cell epitope coding regions in transplant patients. In the current study, we identified 21 human leukocyte antigen (HLA)-A2-positive Spanish patients with chronic hepatitis C, 14 immunosuppressed liver transplant recipients, and 7 immunocompetent controls. Alanine aminotransferase, aspartate aminotransferase, viral load, and rate of fibrosis progression were determined. Genetic distances of HCV isolates and variations in epitopes of the HCV nonstructural 3 protein (NS3-1393 LIFCHSKKK and NS3-1406 KLVALGINAV) were compared between patients with slow or fast progression of fibrosis. Isolates from transplant patients with fast progression were found to be more divergent (P ‫؍‬ .03), had a higher mean value of synonymous (dS) variations (P ‫؍‬ .02), and some were differentiated in a phylogenetic tree, compared with isolates from patients with slow progression. The HLA-A2-restricted NS3-1406 epitope was found to be more variable (20 of 21 isolates differed from the prototype) compared with the A3-restricted NS3-1392 epitope (19% vs. 1.25% variation). A shift in the viral peptide was not detected in a subset of transplant patients, but was evident in two of three nontransplant patients with follow-up. There was no correlation noted between a particular amino acid variation and fibrosis progression (slow or fast) in either transplant or nontransplant patients. The results of the current study suggest that 1) there may be different HCV-1b strains in our geographic area, 2) immunosuppression appears to have little effect in amino acid variation at the HCV NS3-1406 epitope, and 3) variations over time might be more frequent in nonimmunosuppressed patients. (Liver Transpl 2004;10: 217-227.)

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Hepatitis C virus (HCV) genotypes in Spanish patients with HCV infection: relationship between HCV genotype 1b, cirrhosis and hepatocellular carcinoma

Cited by 108 publications

References 32 publications

Nosocomial transmission of HCV in the liver unit of a tertiary care center

Nosocomial transmission of HCV in the liver unit of a tertiary care center

High amino acid variability within the NS5A of hepatitis C virus (HCV) is associated with hepatocellular carcinoma in patients with HCV-1b–related cirrhosis

Genetic variability of hepatitis C virus NS3 protein in human leukocyte antigen-A2 liver transplant recipients with recurrent hepatitis C

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