Hepatic biotransformation enzymes in aquaculture European sea bass (Dicentrarchus labrax): kinetic parameters and induction with benzo(A)pyrene

Lemaire, Philippe; Mathieu, Anne; Carrière, Sylvie; Narbonne, Jean‐François; Lafaurie, M.; Giudicelli, J.

doi:10.1016/0305-0491(92)90203-4

Cited by 14 publications

(8 citation statements)

References 42 publications

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“…Glutathione‐ S ‐transferase activity toward 1‐chloro‐2,4 dinitrobenzene was unaffected by B a P doses up to 10 mg/kg, which is consistent with the literature and previous experiments with dab [21]. Even higher doses up to 50 mg/kg B a P did not affect GST in various other fish species, including English sole ( Pleuronectes vetulus ), flounder ( Platichthys flesus ), and sea bass ( Dicentrarchus labrax ) [26–28]. A power analysis was performed based on the reported variance and the number of animals used.…”

Section: Discussionsupporting

confidence: 89%

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Dose‐ and time‐dependent formation of biliary benzo[a]pyrene metabolites in the marine flatfish DAB (Limanda limanda)

Schanke

Holtz

Meer

et al. 2001

Enviro Toxic and Chemistry

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Polycyclic aromatic hydrocarbons (PAHs) are abundant pollutants, and many PAHs are carcinogenic, but only after metabolic activation. Benzo[a]pyrene (BaP) is among the most carcinogenic PAHs. The dose and time response of two enzymes involved in BaP metabolism and the amounts of BaP metabolites excreted into the bile were evaluated in an experiment with dab (Limanda limanda). Ninety dab were exposed orally to one of five doses of BaP (0, 0.08, 0.4, 2, or 10 mg/kg) and sampled at 3, 6, or 12 d after exposure. None of the doses studied caused significant induction of either microsomal ethoxyresorufin-O-deethylase (EROD). which reflects cytochrome P450 1A (CYP1A) activity, or cytosolic glutathione-S-transferase activity (GST). Concentrations of biliary BaP metabolites significantly increased with dose and significantly decreased with time after exposure. It is concluded that biliary BaP metabolites provide a much more sensitive method than EROD (CYP1A) or GST activity to monitor recent exposure to PAHs in dab.

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Section: Discussionsupporting

confidence: 89%

“…The range of GST activities measured in the present experiment compared well to values reported earlier by other investigators [26][27][28]. Glutathione-S-transferase activity toward 1-chloro-2,4 dinitrobenzene was unaffected by BaP doses up to 10 mg/kg, which is consistent with the literature and previous experiments with dab [21].…”

Section: Enzyme Activitiessupporting

confidence: 93%

Dose‐ and time‐dependent formation of biliary benzo[a]pyrene metabolites in the marine flatfish DAB (Limanda limanda)

Schanke

Holtz

Meer

et al. 2001

Enviro Toxic and Chemistry

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“…Digestive physiology may also play an important role in the assimilation of these dietary PAH chemicals. In contrast, previous studies found that the AE s of the PAH compounds in fish measured using similar methodology were relatively low (<15% for naphthalene and BaP; Corner et al 1976, Fair & Fortner 1987, Lemaire et al 1992). …”

Section: Discussionmentioning

confidence: 54%

Bioaccumulation and transfer of benzo(a)pyrene in a simplified marine food chain

Wang¹,

Wang²

2006

Mar. Ecol. Prog. Ser.

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We examined the toxicokinetics of benzo(a)pyrene (BaP) and the trophic transfer in a marine planktonic food chain comprising phytoplankton, copepods, and fish. Kinetic parameters including the uptake rate from the dissolved phase, the assimilation efficiency (AE) from the ingested food, and the elimination rate were quantified. Influences of food quality and quantity and different routes of exposure (aqueous and food) on the biokinetics were also examined. The uptake rate constants were 390 to 1090 ml mg -1 h -1 in different species of phytoplankton, 1.2 ml mg -1 h -1 in the copepods, and 0.157 ml mg -1 h -1 in the fish, respectively. The assimilation efficiencies were 2 to 24% in the copepods and 32 to 51% in the fish ingesting different prey types. Increasing food concentration significantly reduced the AEs, whereas the AEs varied little among the different food diets tested at the same biomass. The elimination rate constants by the copepods were 0.8 to 1.7 d -1, and comparable following uptake from the aqueous phase and the dietary phase. A kinetic modeling calculation suggests that more BaP accumulated in the copepods originates from the dietary intake than the aqueous intake. For fish, both trophic transfer and aqueous uptake are important in BaP bioaccumulation. Feeding rates contribute to the differences in the relative proportion of accumulated BaP in the fish.

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“…In agreement with Novi et al (1998) "ndings on mature sea bass treated with ip BNF injection, exposure to water-diluted BNF promoted a liver UDP-GT activity increase followed by a decline. The previous results are also supported by earlier studies on other "sh treated with BNF (Anderson et al, 1985;Celander et al, 1997), and on immature sea bass treated with benzo[a]pyrene B(a)P (Lemaire et al, 1992).The sea bass genotoxic response to BNF was assessed using either MN or ENA tests. The idea that BNF is not genotoxic seems to be subjacent to the vast majority of research work carried out.…”

mentioning

confidence: 52%

Liver Phase I and Phase II Enzymatic Induction and Genotoxic Responses of β-Naphthoflavone Water-Exposed Sea Bass

Gravato

Santos

2002

Ecotoxicology and Environmental Safety

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Hepatic biotransformation enzymes in aquaculture European sea bass (Dicentrarchus labrax): kinetic parameters and induction with benzo(A)pyrene

Cited by 14 publications

References 42 publications

Dose‐ and time‐dependent formation of biliary benzo[a]pyrene metabolites in the marine flatfish DAB (Limanda limanda)

Dose‐ and time‐dependent formation of biliary benzo[a]pyrene metabolites in the marine flatfish DAB (Limanda limanda)

Bioaccumulation and transfer of benzo(a)pyrene in a simplified marine food chain

Liver Phase I and Phase II Enzymatic Induction and Genotoxic Responses of β-Naphthoflavone Water-Exposed Sea Bass

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