Heparin/chitosan nanoparticle carriers prepared by polyelectrolyte complexation

Liu, Zonghua; Jiao, Yanpeng; Liu, Fanna; Zhang, Ziyong

doi:10.1002/jbm.a.31407

Cited by 84 publications

(74 citation statements)

References 38 publications

(49 reference statements)

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“…Although the general little or absent effect on physicochemical properties (size and/or zeta potential) upon BSA association is somewhat unexpected, it has been registered in several studies on nanoparticles obtained by polyelectrolyte complexation (de la Fuente et al, 2008b;Grenha et al, 2007;Liu et al, 2007;Vandana and Sahoo, 2009). BSA association with macromolecules has been reported not to be uniform as, to a certain extent, the pattern of association is governed by the 3D conformation that the protein adopts at specific pH and ionic strength.…”

Section: Preparation and Characterisation Of Nanoparticlesmentioning

confidence: 98%

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Dionísio

Cordeiro

Remuñán‐López

et al. 2013

European Journal of Pharmaceutical Sciences

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Polymeric nanoparticles have revealed very effective in transmucosal delivery of proteins. Polysaccharides are among the most used materials for the production of these carriers, owing to their structural flexibility and propensity to evidence biocompatibility and biodegradability. In parallel, there is a preference for the use of mild methods for their production, in order to prevent protein degradation, ensure lower costs and easier procedures that enable scaling up.In this work we propose the production of pullulan-based nanoparticles by a mild method of polyelectrolyte complexation. As pullulan is a neutral polysaccharide, sulfated and aminated derivatives of the polymer were synthesized to provide pullulan with a charge. These derivatives were then complexed with chitosan and carrageenan, respectively, to produce the nanocarriers. Positively charged nanoparticles of 180-270 nm were obtained, evidencing ability to associate bovine serum albumin, which was selected as model protein. In PBS pH 7.4, pullulan-based nanoparticles were found to have a burst release of 30% of the protein, which maintained up to 24h. Nanoparticle size and zeta potential were preserved upon freeze-drying in the presence of appropriate cryoprotectants. A factorial design was approached to assess the cytotoxicity of raw materials and nanoparticles by the metabolic test MTT. Nanoparticles demonstrated to not cause overt toxicity in a respiratory cell model (Calu-3). Pullulan has, thus, demonstrated to hold potential for the production of nanoparticles with an application in protein delivery.

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Section: Preparation and Characterisation Of Nanoparticlesmentioning

confidence: 98%

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Dionísio

Cordeiro

Remuñán‐López

et al. 2013

European Journal of Pharmaceutical Sciences

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“…Materials applied in the production of such chitosan nanoparticles include polymers such as dextran sulfate (Sarmento et al 2006b), sodium alginate (Sarmento et al 2006a, Yang et al 2011, carrageenan (Grenha et al 2010b), arabic gum (Avadi et al 2010), glucomannan (Du et al 2004), carboxymethyl cellulose (Kaihara et al 2011), chondroitin sulfate (Yeh et al 2011), pectin (Grabnar and Kristl 2010), heparin (Liu et al 2007b), hyaluronic acid (de la Fuente et al…”

Section: Ionic Gelation and Polyelectrolyte Complexationmentioning

confidence: 99%

Chitosan nanoparticles: a survey of preparation methods

Grenha¹

2012

Journal of Drug Targeting

238

140

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The application of macromolecules in therapy is frequently hindered by stability and/or permeation issues. These limitations have been addressed by the pharmaceutical industry through the development of suitable non-injectable drug carriers. In this context, nanoparticles have emerged as one of the most exciting tools, due to the increased surface-to-volume ratio, which provides an intimate interaction with epithelial surfaces. Nanoparticles further enable the encapsulated molecules to retain their biological activity, from the production steps to the final release. Chitosan has reached a prominent position as carrier-forming material, as diverse methods can be applied to produce nanoparticles using that excipient. These involve either hydrophilic or lipophilic environments that generally result in mild conditions or aggressive and time-consuming processes, respectively. In this review, a detailed description of methods employed to produce chitosan nanocarriers is provided, accompanied by illustrative schemes of the procedures. The emphasis is on the variables reported to affect the final properties of the vehicles.3

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“…26,27 Heparin is a negatively charged, linear polysaccharide present in many living organisms and a member of the glycosaminoglycan superfamily. 28 The physical adsorption of heparin could prevent early degradation of growth factors, thereby preserving their biological activity. With its capacity for antithromobogenicity, heparin is used for suppressing acute subthrombus.…”

Section: Introductionmentioning

confidence: 99%

“…[29][30][31] Thus, a chitosan and heparin nanoparticle provides a large loading capacity while stimulating regeneration and preventing early degredation. 28 In this study, our main objective is to develop a scaffold with large loading capacity, retention of biological activity, and a sustained local release of VEGF to enhance the vascularization of decellularized bovine jugular vein scaffolds. The large loading capacity was established by the application of nanoparticles to localize VEGF.…”

Section: Introductionmentioning

confidence: 99%

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Controlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds

Tan¹,

Tang²,

Hu³

et al. 2011

IJN

111

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Regeneration deficiency is one of the main obstacles limiting the effectiveness of tissue-engineered scaffolds. To develop scaffolds that are capable of accelerating regeneration, we created a heparin/chitosan nanoparticle-immobilized decellularized bovine jugular vein scaffold to increase the loading capacity and allow for controlled release of vascular endothelial growth factor (VEGF). The vascularization of the scaffold was evaluated in vitro and in vivo. The functional nanoparticles were prepared by physical self-assembly with a diameter of 67–132 nm, positive charge, and a zeta potential of ∼30 mV and then the nanoparticles were successfully immobilized to the nanofibers of scaffolds by ethylcarbodiimide hydrochloride/hydroxysulfosuccinimide modification. The scaffolds immobilized with heparin/chitosan nanoparticles exhibited highly effective localization and sustained release of VEGF for several weeks in vitro. This modified scaffold significantly stimulated endothelial cells’ proliferation in vitro. Importantly, utilization of heparin/chitosan nanoparticles to localize VEGF significantly increased fibroblast infiltration, extracellular matrix production, and accelerated vascularization in mouse subcutaneous implantation model in vivo. This study provided a novel and promising system for accelerated regeneration of tissue-engineering scaffolds.

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Heparin/chitosan nanoparticle carriers prepared by polyelectrolyte complexation

Cited by 84 publications

References 38 publications

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Chitosan nanoparticles: a survey of preparation methods

Controlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds

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