Heparanase overexpression down-regulates syndecan-1 expression in a gallbladder carcinoma cell line

Jin, Hao; Zhou, Shuping; Yang, Song; Cao, Haiming

doi:10.1177/0300060517700323

Cited by 6 publications

(4 citation statements)

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“…The variables included in Module 1 are as follows: HPSE1 (main predictor), Sdc1–4 (intermediate predictors), and HS10E4 (main outcome; main predictor/link to module 2) ( Figure 5 A,B). The SplR pairs were modeled according to the well-documented role of HPSE1 as an endoglycosidase enzyme that cleaves HS GAG chains and facilitates shedding of the extracellular domains of Sdc [ 27 , 28 , 29 ]. Therefore, VKO or the absence of HPSE1 (−100% of baseline expression measured in diseased gingiva) is predicted to increase the expression of HS GAG and Sdcs.…”

Section: Resultsmentioning

confidence: 99%

Heparan Sulfate Glycosaminoglycan Is Predicted to Stabilize Inflammatory Infiltrate Formation and RANKL/OPG Ratio in Severe Periodontitis in Humans

et al. 2022

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Since chronically inflamed periodontal tissue exhibits extracellular matrix (ECM) degradation, the possible alternative to standard periodontitis treatment is to restore ECM by supplementing its components, including heparan sulfate glycosaminoglycan (HS GAG). Supplementation of the degraded ECM with synthetic derivatives of HS GAGs has been shown to be effective for periodontal tissue regeneration in experimental animal models of periodontitis. However, the potential of HS GAG supplementation for the treatment of periodontal disease in humans is still unknown. Here, we used a statistical model to investigate the role of HS GAG on inflammatory infiltrate formation and alveolar bone resorption in humans with severe periodontitis. The model was based on data from immunofluorescence staining (IF) of human gingiva samples, and reconstruction of a subset of HS GAG -related proteins from STRING reactome database. According to predictions, increased expression of native HS GAG might stabilize the accumulation of gingival inflammatory infiltrate (represented by the general inflammatory cell marker CD45) and alveolar bone resorption (represented by Receptor Activator of Nuclear ΚΒ ligand (RANKL) and osteoprotegerin (OPG) ratio) but could not restore them to healthy tissue levels. Therefore, supplementation of native HS GAG may be of limited benefits for the treatment of sever periodontitis in humans.

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Section: Resultsmentioning

confidence: 99%

Heparan Sulfate Glycosaminoglycan Is Predicted to Stabilize Inflammatory Infiltrate Formation and RANKL/OPG Ratio in Severe Periodontitis in Humans

et al. 2022

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“…In addition, syndecan‐1 gene knockdown can promote the proliferation, invasion and metastasis of GBC cells by regulating ERK/Snail signal transduction and inducing EMT and cancer cells invasion. 73 , 74 However, whether miR‐433 plays a similar role in GBC as in other malignant tumors of digestive tract needs to be identified in the future experiments.…”

Section: Discussionmentioning

confidence: 99%

The role of MiRNA‐433 in malignant tumors of digestive tract as tumor suppressor

et al. 2022

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Background MicroRNAs (miRNAs) are a class of short non‐coding RNAs with a length of approximate 22 nuclei acids that can be expressed both as an oncogene and tumor suppressor gene in human cancers. MiRNAs can participate in the post‐ transcriptional regulation of gene expression, and regulate the several cancer‐related processes, including proliferation, apoptosis, metastasis, etc. Recent findings Expression of miRNA‐433 has been reported to vary in different tumors and affected by various factors. We have summarized the different previous studies and found that miRNA‐433 can significantly inhibit the growth of the cancer cells not only in malignant tumors of the digestive tract, but also in lung cancer, breast cancer, cervical cancer, ovarian cancer, bladder cancer, renal carcinoma, glioma, retinoblastoma and osteosarcoma. Conclusion When the expression of miRNA‐433 was up‐regulated, the proliferation, metastasis and invasion abilities of the malignant tumor cells were significantly inhibited. At the same time, the potential mechanisms through which miRNA‐433 can suppress the growth and metastasis of the cancer cells were found to be basically the same, and involved modulation of the specific signaling pathways or target genes in the malignant tumors. Overall, it can be concluded that miRNA‐433 can serve as potential and valuable therapeutic target.

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“…In the PPI network analysis, the epithelial growth factor receptor (EGFR), a vital role in cancer growth and development, lies in the center of the network [28][29][30]. Since the role of HMA in cancer inhibition is complex (Figure 9), further work should be done.…”

Section: Discussionmentioning

confidence: 99%

Recognition of potential therapeutic role of 2‐hydroxy‐3‐methylanthraquinones in the treatment of gallbladder carcinoma: A proteomics analysis

Jin

Cui

2021

Fundamemntal Clinical Pharma

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Gallbladder carcinoma (GBC), with early metastasis and high recurrence rates, is an enormous threat to health. As an anthraquinones monomer of traditional Chinese medicine Hedyotis diffusa, 2hydroxy-3-methylanthraquinone (HMA) has been reported to inhibit the growth of several cancers. But in our preliminary study, HMA could only weakly induce GBC cell apoptosis. To explore other possible mechanism underlying the inhibition effect of HMA on GBC, this proteomics analysis was performed. A proteomics analysis was performed on one GBC cell line bought from the China Life Science Cell Bank. Several computational techniques were merged to develop analysis for those differently expressed proteins. A comparative protein-protein interaction network analysis was carried out among the differently expressed proteins to identify the proteins potentially inhibiting GBC. Thus, a GO and KEGG analysis was performed to identify the signaling pathways underlying a potential therapeutic role for HMA. A total of 285 proteins were affected by HMA, including 187 upregulated and 98 downregulated. The subcellular localization of differently expressed proteins were identified, including 142 in nuclear, 67 in cytoplasm, 67 in extracellular matrix, 46 in plasma membrane, 13 in mitochondrion, 3 in lysosome, and 1 in cytoskeleton. HMA could regulate EGFR, FN1, PLG, PLAUR, LAMA3, HRG, THBS1, PLAT, KNG1, ENAM, SERPINE1, ECM1, interleukin-8, and trypsin in GBC. Most of the regulated proteins involve in cell migration. Pathways including PI3K-Akt, Wnt, HIF-1, focal adhesion, microRNAs were regulated by HMA. HMA was shown to be an inhibition agent for GBC development, and this analysis would contribute to the development of new anti-GBC drugs.K E Y W O R D S 2-hydroxy-3-methylanthraquinones, gallbladder carcinoma, Hedyotis diffusa, proteomics, traditional Chinese medicine | INTRODUCTIONIn selected areas of high incidence including India (21.5/100 000), Chile (18.1/100 000), Pakistan (13.8/100 000), and Ecuador (12.9/100 000), gallbladder carcinoma (GBC) is a significant source of mortality [1]. High incidences have also been found in Korea, Japan, Poland, the Czech Republic, and Slovakia [2].

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Heparanase overexpression down-regulates syndecan-1 expression in a gallbladder carcinoma cell line

Cited by 6 publications

References 50 publications

Heparan Sulfate Glycosaminoglycan Is Predicted to Stabilize Inflammatory Infiltrate Formation and RANKL/OPG Ratio in Severe Periodontitis in Humans

Heparan Sulfate Glycosaminoglycan Is Predicted to Stabilize Inflammatory Infiltrate Formation and RANKL/OPG Ratio in Severe Periodontitis in Humans

The role of MiRNA‐433 in malignant tumors of digestive tract as tumor suppressor

Recognition of potential therapeutic role of 2‐hydroxy‐3‐methylanthraquinones in the treatment of gallbladder carcinoma: A proteomics analysis

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