Hemozoin “knobs” in Opisthorchis felineus infected liver

Pershina, Alexandra G.; Saltykova, Irina V.; Ivanov, V. V.; Perina, Ekaterina A.; Demin, A. M.; Shevelev, Oleg B.; Бузуева, И. И.; Гутаковский, А. К.; Vtorushin, S. V.; Ганебных, И. Н.; Краснов, В. П.; Сазонов, А. Э.; Огородова, Л. М.

doi:10.1186/s13071-015-1061-5

Cited by 15 publications

(14 citation statements)

References 42 publications

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“…The parasites detoxify this waste product by biocrystallization with the enzyme heme oxygenase, which breaks down heme into iron, biliverdin and carbon monoxide . The paramagnetic effect of hemozoin in the liver is strong enough to be detected by MRI .…”

Section: Iron Compounds and Their Susceptibilitymentioning

confidence: 99%

Iron quantification with susceptibility

Ropele

Langkammer

2016

NMR in Biomedicine

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Iron is an essential trace element involved in a variety of biological mechanisms in the human body. Disturbances of iron homeostasis have been observed in several inflammatory and degenerative diseases, which have raised strong interest in non-invasive iron mapping techniques. Numerous MRI techniques have been proposed so far, mostly based on the field changes induced by the magnetic properties of iron. Each of these approaches has a specific sensitivity for iron and its microstructural environment. Quantitative susceptibility mapping is the latest development and provides a direct measure of bulk susceptibility. However, field changes induced by iron are not always directly related to the concentration of iron, but rather reflect the structure of iron compounds and its cellular distribution. This review provides an overview of the most relevant iron compounds in the human body, their magnetic properties and their cellular distribution. In addition, MRI methods based on direct or indirect susceptibility changes are presented and discussed with respect to technical aspects and clinical applicability. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Iron Compounds and Their Susceptibilitymentioning

confidence: 99%

Iron quantification with susceptibility

Ropele

Langkammer

2016

NMR in Biomedicine

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“…33 Hamsters were housed three to a cage (OptiRAT) under conventional conditions and were fed with stock diet and water ad libitum. The hamsters at the age of 30 weeks and with the weight of ~100 g were used for the MRI experiments (n=6).…”

Section: Experimental Animal Modelmentioning

confidence: 99%

3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by <em>Opisthorchis felineus</em>

Demin¹,

Pershina²,

Ivanov³

et al. 2016

IJN

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Purpose Liver fluke causes severe liver damage in an infected human. However, the infection often remains neglected due to the lack of pathognomonic signs. Nanoparticle-enhanced magnetic resonance imaging (MRI) offers a promising technique for detecting liver lesions induced by parasites. Materials and methods Surface modification of iron oxide nanoparticles produced by coprecipitation from a solution of Fe 3+ and Fe 2+ salts using 3-aminopropylsilane (APS) was carried out. The APS-modified nanoparticles were characterized by transmission electron microscopy, fourier transform infrared spectroscopy, and thermogravimetric analysis. Magnetic resonance properties of MNPs were investigated in vitro and in vivo. Results The amount of APS grafted on the surface of nanoparticles (0.60±0.06 mmol g −1 ) was calculated based on elemental analysis and infrared spectroscopy data. According to transmission electron microscopy data, there were no essential changes in the structure of nanoparticles during the modification. The APS-modified nanoparticles exhibit high magnetic properties; the calculated relaxivity r 2 was 271 mmol −1 s −1 . To obtain suspension with optimal hydrodynamic characteristics, amino groups on the surface of nanoparticles were converted into an ionic form with HCl. Cellular uptake of modified nanoparticles by rat hepatoma cells and human monocytes in vitro was 74.1±4.5 and 10.0±3.7 pg [Fe] per cell, respectively. Low cytotoxicity of the nanoparticles was confirmed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Annexin V/7-aminoactinomycin D flow cytometry assays. For the first time, magnetic nanoparticles were applied for contrast-enhanced MRI of liver lesions induced by Opisthorchis felineus . Conclusion The synthesized APS-modified iron oxide nanoparticles showed high efficiency as an MRI contrast agent for the evaluation of opisthorchiasis-related liver damage.

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“…It is little known about the main source and proportion of nutrients for O . felineus [ 37 , 64 ], but liver flukes inhabiting the bile ducts are likely able to uptake nutrients from the bile. Young et al reported [ 65 ] that closely related species O .…”

Section: Discussionmentioning

confidence: 99%

“…166 “About fisheries and conservation of water biological resources” from 20.12.2004. For the experiment, 5-week-old male hamsters (n = 8) ( Mesocricetus auratus ) were infected intragastrically with 50 metacercariae per hamster, according to the previously described protocol [ 37 ]. Age-matched intact male hamsters (n = 8) were used as controls.…”

Section: Methodsmentioning

confidence: 99%

Magnetic resonance imaging and spectroscopy for differential assessment of liver abnormalities induced by Opisthorchis felineus in an animal model

et al. 2017

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BackgroundEuropean liver fluke Opisthorchis felineus, causing opisthorchiasis disease, is widespread in Russia, Ukraine, Kazakhstan and sporadically detected in the EU countries. O. felineus infection leads to hepatobiliary pathological changes, cholangitis, fibrosis and, in severe cases, malignant transformation of bile ducts. Due to absence of specific symptoms, the infection is frequently neglected for a long period. The association of opisthorchiasis with almost incurable bile duct cancer and rising international migration of people that increases the risk of the parasitic etiology of liver fibrosis in non-endemic regions determine high demand for development of approaches to opisthorchiasis detection.Methodology/Principal findingsIn vivo magnetic resonance imaging and spectroscopy (MRI and MRS) were applied for differential assessment of hepatic abnormalities induced by O. felineus in an experimental animal model. Correlations of the MR-findings with the histological data as well as the data of the biochemical analysis of liver tissue were found. MRI provides valuable information about the severity of liver impairments induced by opisthorchiasis. An MR image of O. felineus infected liver has a characteristic pattern that differs from that of closely related liver fluke infections. 1H and 31P MRS in combination with biochemical analysis data showed that O. felineus infection disturbed hepatic metabolism of the host, which was accompanied by cholesterol accumulation in the liver.ConclusionsA non-invasive approach based on the magnetic resonance technique is very advantageous and may be successfully used not only for diagnosing and evaluating liver damage induced by O. felineus, but also for investigating metabolic changes arising in the infected organ. Since damages induced by the liver fluke take place in different liver lobes, MRI has the potential to overcome liver biopsy sampling variability that limits predictive validity of biopsy analysis for staging liver fluke-induced fibrosis.

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Hemozoin “knobs” in Opisthorchis felineus infected liver

Cited by 15 publications

References 42 publications

Iron quantification with susceptibility

Iron quantification with susceptibility

3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by <em>Opisthorchis felineus</em>

Magnetic resonance imaging and spectroscopy for differential assessment of liver abnormalities induced by Opisthorchis felineus in an animal model

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