Hemogram study of an artificially feeding tree shrew (<i>Tupaia belangeri chinensis</i>)

Abstract

Systematic classification and determination of various cells in normal peripheral blood of artificially feeding Tupaia belangeri chinensis of different ages and genders and evaluation of the effectiveness of an automatic blood cell classification counter for measuring tree shrew blood cells. Child, young and adult tree shrews (forty for each group) were randomly selected, half male and half female. After the animals were stable, the peripheral blood of each group was collected through the femoral vein, and the morphology of various blood cells of the tree shrew was observed and classified by the manual microscopic counting method and by an automatic blood cell classification counter. The Reference intervals of the normal peripheral blood cell absolute count, cell diameter and white blood cell percentage in tree shrews of different ages and genders has been calculated. White blood cell count and neutrophil relative count increased with age, while lymphocyte relative count decreased. The white blood cell count, neutrophil relative count, and lymphocyte relative count in the child group, as well as lymphocyte relative count in the young group, significantly differed according to gender (P<0.05), and the differences in other indicators were not significant. The Bland-Altman plot and the Passing-Bablok scattergram showed that the change trend of each indicator was consistent but exhibited large systematic differences between methods. Differences in peripheral blood cells exist among different age groups and different genders. An automatic blood cell classification counter is not suitable for the absolute count of blood cells in the tree shrew.

AA-amyloidosis in captive northern tree shrews (Tupaia belangeri)

“…The qualitative and semiquantitative urinalysis revealed leucocytes and elevated levels of urobilinogen, ketones, bilirubin, protein, and glucose. Compared with available reference values for Tupaia spp., 10,11,15,22,34,38,41,42 the hematological and biochemical profiles had low concentrations of alkaline phosphatase, aspartate aminotransferase, and creatine kinase; elevated creatinine, chloride, and glucose; and reduced albumin and total protein levels. The calcium-to-phosphorus ratio was 2.1:2.7 (Supplemental Table S4).…”

The Tree Shrew as a Model for Cancer Research

“…The values for leukocytes (2.21 × 10 9 /L) and neutrophils (0.85 × 10 9 /L) are below the lower limit of the human reference value. The lymphocytes, eosinophils, basophils and monocytes of tree shrews are generally within the reference range for humans ( 36 – 38 ). Biochemical analysis showed that only the creatinine results for tree shrews are below the lower limit of the human reference value, while the alanine aminotransferase and aspartate transaminase values are above the upper limit of the reference value.…”

The tree shrew as a new animal model for the study of periodontitis

“…Immunological processes of tree shrews correlate strongly with the disease progression (Parkin & Cohen, 2001). The immune system of tree shrews is similar to that of humans, including the similar neutrophil percentage and similar morphology and size of erythrocytes (Feng et al, 2020). Zhang et al found, for the first time in 2020, that the serum of tree shrews immunized with polysaccharides could induce antibody‐dependent bactericides, suggesting that the immune response of humans is more like that of tree shrews than of rodents (Zhang et al, 2020).…”