Hemocompatibility and oxygenation performance of polysulfone membranes grafted with polyethylene glycol and heparin by plasma-induced surface modification

Abstract

Polyethylene glycol (PEG) and heparin (Hep) were grafted onto polysulfone (PSF) membrane by plasma-induced surface modification to prepare PSF-PEG-Hep membranes used for artificial lung. The effects of plasma treatment parameters, including power, gas type, gas flow rate, and treatment time, were investigated, and different PEG chains were bonded covalently onto the surface in the postplasma grafting process. Membrane surfaces were characterized by water contact angle, PEG grafting degree, attenuated total reflectance-Fourier transform infrared spectroscopy, ultraviolet-visible spectrophotometry, X-ray photoelectron spectroscopy, critical water permeability pressure, and scanning electron microscopy. Protein adsorption, platelet adhesion, and coagulation tests showed significant improvement in the hemocompatibility of PSF-PEG-Hep membranes compared to pristine PSF membrane. Gas exchange tests through PSF-PEG6000-Hep membrane showed that when the flow rate of porcine blood reached 5.0 L/min, the permeation fluxes of O and CO reached 192.6 and 166.9 mL/min, respectively, which were close to the gas exchange capacity of a commercial membrane oxygenator. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1737-1746, 2017.

Hemocompatibility of hyaluronan enhanced linear low density polyethylene for blood contacting applications

“…Thus, to understand how a surface may be affecting hemocompatibility it is essential to understand how the many components of the coagulation cascade are being influenced by its surface properties. Exploration of HA enhanced surfaces suggest that HA can subdue the activation of the hemostatic response 12,14,19,22,23 . In this study, we characterize the HA-LLDPE surface and investigate its effects on hemolysis, cytocompatibility, protein adhesion, cellular adhesion, and specific biochemical markers to gain a thorough understanding of the hematological response.…”

“…To mitigate these coagulation agonists, the manipulation of biomaterial surfaces has been widely investigated in hopes of achieving enhanced hemocompatibility of biomaterials. Some examples to enhance hemocompatibility include plasma surface modification, the grafting of oligo and poly(ethylene glycol)s (OEG, PEG), zwitterionic structures, heparin modification, the fabrication of PEGylated terapolymers, and the endothelialization of biomaterial surfaces 1,3,7–19 Among the many techniques proposed to enhance hemocompatibility, much success has been found through the exploration of hyaluronan enhanced surfaces which have been shown to placate the hemostatic response 12,14,20–24 .…”

“…Some examples to enhance hemocompatibility include plasma surface modification, the grafting of oligo and poly(ethylene glycol)s (OEG, PEG), zwitterionic structures, heparin modification, the fabrication of PEGylated terapolymers, and the endothelialization of biomaterial surfaces. 1,3,[7][8][9][10][11][12][13][14][15][16][17][18][19] . Among the many techniques proposed to enhance hemocompatibility, much success has been found through the exploration of hyaluronan (HA) enhanced surfaces which have been shown to placate the hemostatic response.…”

Surface modification of polysulfone hollow fiber membrane for extracorporeal membrane oxygenator using low‐temperature plasma treatment

“…PSF HFMs with average pore size of 0.1 μm, porosity of 60%, outer diameter of 900 μm, and inner diameter of 700 μm were prepared using non‐solvent‐induced phase separation . This method of preparation was also used in our previously published article . AA was purchased from Nanjing Chemical Reagent Company, China.…”

“…In our previous work, we had conducted relevant research on membrane material preparation for membrane oxygenators. Acrylic acid (AA) and PEG were grafted to the surface of PSF plate membranes to prepare PSF‐AA‐PEG membrane . 2‐Methacryloyloxyethyl phosphorylcholine (MPC) and heparin were grafted to polymethylpentene (PMP) membrane surface to prepare PMP‐MPC and PMP‐Hep membrane, respectively .…”