Hemagglutinating Virus of Japan Envelope Vectors as High-Performance Vehicles for Delivery of Small RNAs

Kato, Fuminori; Yagi, Takaharu; Fujieda, Takayuki; Kondo, Yoshitaka; Yamaguchi, Tomona; Miyata, Keizo; Kaneda, Yasufumi

doi:10.4172/2157-7412.1000178

Cited by 3 publications

(3 citation statements)

References 94 publications

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“…This haemagglutinating virus of Japan envelope (HVJ‐E; GenomeONE‐Neo EX HVJ‐E; Cosmo Bio Co., Ltd, Koto‐ku, Tokyo, Japan) is a replication‐incompetent vector developed from the Sendai virus . The viral genome is inactive, and only the cell membrane fusion properties are intact, allowing effective delivery of siRNAs into cells without eliciting a cytotoxic response .…”

Section: Methodsmentioning

confidence: 99%

Masculinisation of the Zebra Finch Song System: Roles of Oestradiol and the Z‐chromosome Gene Tubulin‐Specific Chaperone Protein A

Beach

Wade

2015

J Neuroendocrinology

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Robust sex differences in brain and behaviour exist in zebra finches. Only males sing, and forebrain song control regions are more developed in males. The factors driving these differences are not clear, although numerous experiments have shown that oestradiol (E2) administered to female hatchlings partially masculinises brain and behaviour. Recent studies suggest that an increased expression of Z-chromosome genes in males (ZZ; females: ZW) might also play a role. The Z-gene tubulin-specific chaperone A (TBCA) exhibits increased expression in the lateral magnocellular nucleus of the anterior nidopallium (LMAN) of juvenile males compared to females; TBCA+ cells project to the robust nucleus of the arcopallium (RA). In the present study, we investigated the role of TBCA and tested hypotheses with respect to the interactive or additive effects of E2 and TBCA. We first examined whether E2 in hatchling zebra finches modulates TBCA expression in the LMAN. It affected neither the mRNA, nor protein in either sex. We then unilaterally delivered TBCA small interfering (si)RNA to the LMAN of developing females treated with E2 or vehicle and males treated with the aromatase inhibitor, fadrozole, or its control. In both sexes, decreasing TBCA in LMAN reduced RA cell number, cell size and volume. It also decreased LMAN volume in females. Fadrozole in males increased LMAN volume and RA cell size. TBCA siRNA delivered to the LMAN also decreased the projection from this brain region to the RA, as indicated by anterograde tract tracing. The results suggest that TBCA is involved in masculinising the song system. However, because no interactions between the siRNA and hormone manipulations were detected, TBCA does not appear to modulate effects of E2 in the zebra finch song circuit.

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Section: Methodsmentioning

confidence: 99%

Masculinisation of the Zebra Finch Song System: Roles of Oestradiol and the Z‐chromosome Gene Tubulin‐Specific Chaperone Protein A

Beach

Wade

2015

J Neuroendocrinology

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“…The HVJ‐E vector (hemagglutinin virus of Japan‐envelope) has been shown to rapidly deliver proteins and oligonucleotides into BHK21 cells by membrane fusion . To confer target‐specificity and safety to these particles, HVJ‐E was coupled with maghemite MNPs.…”

Section: Polymer–viral Vector Hybrid Nanoparticlesmentioning

confidence: 99%

Polymer‐coated viral vectors: hybrid nanosystems for gene therapy

Rajagopal

Duraiswamy

Sethuraman

et al. 2018

The Journal of Gene Medicine

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The advantages and critical aspects of nanodimensional polymer-coated viral vector systems potentially applicable for gene delivery are reviewed. Various viral and nonviral vectors have been explored for gene therapy. Viral gene transfer methods, although highly efficient, are limited by their immunogenicity. Nonviral vectors have a lower transfection efficiency as a result of their inability to escape from the endosome. To overcome these drawbacks, novel nanotechnology-mediated interventions that involve the coating or modification of virus using polymers have emerged as a new paradigm in gene therapy. These alterations not only modify the tropism of the virus, but also reduce their undesirable interactions with the biological system. Also, co-encapsulation of other therapeutic agents in the polymeric coating may serve to augment the treatment efficacy. The viral particles can aid endosomal escape, as well as nuclear targeting, thereby enhancing the transfection efficiency. The integration of the desirable properties of both viral and nonviral vectors has been found beneficial for gene therapy by enhancing the transduction efficiency and minimizing the immune response. However, it is essential to ensure that these attempts should not compromise on the inherent ability of viruses to target and internalize into the cells and escape the endosomes.

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“…This method of siRNA transfection and delivery has been successfully used in developing zebra finches (Beach and Wade, 2015). The viral genome of HVJ-E is inactive, with only the cell membrane fusion properties intact, allowing effective delivery of siRNAs into cells without eliciting a cytotoxic response (Kato et al, 2013). …”

Section: Methodsmentioning

confidence: 99%

Inhibition of TrkB limits development of the zebra finch song system

et al. 2016

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Large sexual dimorphisms exist in the zebra finch song system. Masculinization may be mediated by both estradiol and expression of one or more Z-genes (males: ZZ; females: ZW). Roles of the Z-gene tyrosine kinase B (TrkB) in HVC in masculinizing both it and its target the robust nucleus of the arcopallium (RA) were tested using siRNA administration in juvenile males at two ages (post-hatching days 15-17 or 25-27). Birds were euthanized 10 days later. Potential interactions or additive effects with estradiol were evaluated by treating males with the estrogen synthesis inhibitor fadrozole. Females treated with estradiol were also exposed to the siRNA at the later age. Local inhibition of TrkB in males of both ages reduced the volume of HVC, an effect due to a change in cell number and not cell size. In the older males, in which the treatment spanned the period when the projection from HVC to RA grows, TrkB inhibition reduced the volume of RA and the relative number of cells within it. TrkB siRNA in HVC decreased the volume of and soma size in the RA of females, and the projection from HVC to RA in both sexes. Estradiol in females masculinized various aspects of the song system, and its effect in masculinizing the volume of RA was decreased by TrkB inhibition. However, effects of fadrozole in males were limited. The data indicate that TrkB is involved in masculinizing the song system, but for most measures it probably does not work in concert with E2.

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Hemagglutinating Virus of Japan Envelope Vectors as High-Performance Vehicles for Delivery of Small RNAs

Cited by 3 publications

References 94 publications

Masculinisation of the Zebra Finch Song System: Roles of Oestradiol and the Z‐chromosome Gene Tubulin‐Specific Chaperone Protein A

Masculinisation of the Zebra Finch Song System: Roles of Oestradiol and the Z‐chromosome Gene Tubulin‐Specific Chaperone Protein A

Polymer‐coated viral vectors: hybrid nanosystems for gene therapy

Inhibition of TrkB limits development of the zebra finch song system

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