Helminths of roe deer (Capreolus capreolus) in the Middle Black Sea Region of Turkey

Bölükbaş, Cenk Soner; Gürler, Ali Tümay; Beyhan, Yunus Emre; Açıcı, Mustafa; Umur, Şinasi

doi:10.1016/j.parint.2012.06.008

Cited by 30 publications

(21 citation statements)

References 8 publications

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“…The identified parasites were in accordance with data collected by other authors from Switzerland and other countries [11,83–86]. This indicates that endoparasitosis is common in roe deer and not necessarily clinically significant, but it may contribute to the disease process when challenging environmental conditions or other weakening causes occur.…”

Section: Discussionsupporting

confidence: 88%

“…Furthermore, the fact that Chabertia ovina and small gastric worms have disappeared from the dataset are likely not only due to changes in the laboratory methods but also to a loss of knowledge over time. This hypothesis is supported by recent studies which have documented these parasites in roe deer from other countries [83,84,86]. Thus, targeted investigations are required to assess the current occurrence of gastric helminths and their importance in pathological processes in roe deer in Switzerland.…”

Section: Discussionmentioning

confidence: 75%

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Assessing Fifty Years of General Health Surveillance of Roe Deer in Switzerland: A Retrospective Analysis of Necropsy Reports

et al. 2017

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General wildlife health surveillance is a valuable source of information on the causes of mortality, disease susceptibility and pathology of the investigated hosts and it is considered to be an essential component of early warning systems. However, the representativeness of data from such surveillance programs is known to be limited by numerous biases. The roe deer (Capreolus capreolus capreolus) is the most abundant ungulate and a major game species all over Europe. Yet, internationally available literature on roe deer pathology is scarce. The aims of this study were (1) to provide an overview of the causes of mortality or morbidity observed in roe deer in Switzerland and to assess potential changes in the disease pattern over time; and (2) to evaluate the value and limitations of a long term dataset originating from general wildlife health surveillance. We compiled 1571 necropsy reports of free ranging roe deer examined at the Centre for Fish and Wildlife Health in Switzerland from 1958 to 2014. Descriptive data analysis was performed considering animal metadata, submitter, pathologist in charge, laboratory methods, morphological diagnoses and etiologies. Recurrent causes of mortality and disease pictures included pneumonia, diarrhea, meningoencephalitis, actinomycosis, blunt trauma, predation, neoplasms and anomalies. By contrast, other diagnoses such as fatal parasitic gastritis, suspected alimentary intoxication and reproductive disorders appeared only in earlier time periods. Diseases potentially relevant for other animals or humans such as caseous lymphadenitis (or pseudotuberculosis), salmonellosis, paratuberculosis and listeriosis were sporadically observed. The disease pattern in roe deer from Switzerland was largely in accordance with previous reports. The observed fluctuations were consistent with methodical and/or personnel changes and varying disease awareness. Nevertheless, despite such limitations, the compiled data provide a valuable baseline. To facilitate comparison among studies, we recommend systematically archiving all case documents and fixed tissues and to perform data analyses more regularly and in a harmonized way.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 75%

Assessing Fifty Years of General Health Surveillance of Roe Deer in Switzerland: A Retrospective Analysis of Necropsy Reports

et al. 2017

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“…As outlined above there are also several other species listed herein that are primarily parasites of sheep, that are generalist known to infect a wide range of wildlife ungulates in Europe [7]. Not at least roe-deer in Spain [34], Italy [35], Turkey [36], and France [37], is known to be infected with several of the species identi ed herein. Among these, particularly H. contortus is the most pathogenic nematode of sheep in Sweden [3] and is therefore of major interest.…”

Section: Discussionmentioning

confidence: 88%

Sheep Nemabiome Diversity and Its Response to Anthelmintic Treatment in Swedish Sheep Herds

Halvarsson¹,

Höglund

2020

Preprint

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Background: A novel way to study the species composition and diversity of nematode parasites in livestock is to perform deep sequencing on composite samples containing a mixture of different species. Herein we describe for the first-time the nematode community structure (nemabiome) inhabiting Swedish sheep and how these are/were affected by host age and recent anthelmintic treatments. Methods: A total of 158 larval cultures were collected (n=35 in 2007 and n=126 in 2014-2016) from groups of sheep on 61 commercial farms in the south-central part of country were most animals are grazed. Among the samples, 2 x 44 (56%) were paired collected from the same groups pre- and post-treatment with macrocyclic lactones, benzimidazoles or levamisole. The sequences were analyzed for their nemabiome using the PacBio platform followed by bioinformatic sequence analysis with SCATA. Species richness and diversity were calculated and analyzed in R.Results: Nematode ITS2 sequences were found in all samples except two, despite that the fecal egg counts were below the McMaster threshold in 20 samples. Sequencing yielded on average 1,011 sequences per sample. Totally 26 operational taxonomical units (OTU) among which 18 (69%) had ≥99 % identity to sequences in the NCBI database were recognized. The OTUs found represented nematode species among which 10 are commonly associated with sheep. Multiple species were identified in all pre anthelmintic treatment samples. No effects on nematode diversity were found in relation to host age. On the other hand, recent anthelmintic treatment lowered species richness, especially after use of IVM and ABZ. Interestingly, despite zero egg count after use of levamisole, these samples still contained nematode DNA and especially H. contortus. Conclusions: Our findings provide evidence for that nemabiome analysis combined with diversity index analysis provide a sensitive and objective methodology in the study of the efficacy of anthelmintic treatment.

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“…Interestingly, we recognized no species primarily associated with wildlife., However, there are generalist known to infect a wide range of wildlife ungulates in Europe [7]. Not at least roe-deer in Spain [38], Italy [39], Turkey [40], and France [41], is known to be infected with species identi ed herein. Among these, particularly H. contortus is the most pathogenic nematode of sheep in Sweden [3] and is therefore of major interest.…”

Section: Discussionmentioning

confidence: 96%

Sheep nemabiome diversity and its response to anthelmintic treatment in Swedish sheep herds

Halvarsson¹,

Höglund

2020

Preprint

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BackgroundA novel way to study the species composition and diversity of nematode parasites in livestock is to perform deep sequencing on composite samples containing a mixture of different species. Herein we describe for the first-time the nematode community structure (nemabiome) inhabiting Swedish sheep and how these are/were affected by host age and recent anthelmintic treatments.MethodsA total of 158 fecal samples were collected (n=35 in 2007 and n=123 in 2013-2016) and cultured from groups of sheep on 61 commercial farms in the south-central part of country where most animals are grazed. Among the samples, 2 x 44 (56%) were paired collections from the same groups pre- and post-treatment with macrocyclic lactones, benzimidazoles or levamisole. The sequences were analyzed for their nemabiome using the PacBio platform followed by bioinformatic sequence analysis with SCATA. Species richness and diversity were calculated and analyzed in R.ResultsNematode ITS2 sequences were found in all larval culture samples except two, even though the fecal egg counts were below the McMaster threshold in 20 samples. Sequencing yielded, on average, 1,008 sequences per sample. In total 16 operational taxonomical units (OTU), all had ≥98 % identity to sequences in the NCBI database, were recognized. The OTUs found represented nematode species of which 10 are commonly associated with sheep. Multiple species were identified in all pre anthelmintic treatment larval culture samples. No effects on nematode diversity were found in relation to host age. On the other hand, recent anthelmintic treatment lowered species richness, especially after use of ivermectin and albendazol. Interestingly, despite zero egg counts after use of levamisole, these samples still contained nematode DNA and especially H. contortus.ConclusionsOur findings provide evidence that nemabiome analysis combined with diversity index analysis provide a sensitive and objective methodology in the study of the efficacy of anthelmintic treatment, as both high and low abundant species were detected.

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Helminths of roe deer (Capreolus capreolus) in the Middle Black Sea Region of Turkey

Cited by 30 publications

References 8 publications

Assessing Fifty Years of General Health Surveillance of Roe Deer in Switzerland: A Retrospective Analysis of Necropsy Reports

Assessing Fifty Years of General Health Surveillance of Roe Deer in Switzerland: A Retrospective Analysis of Necropsy Reports

Sheep Nemabiome Diversity and Its Response to Anthelmintic Treatment in Swedish Sheep Herds

Sheep nemabiome diversity and its response to anthelmintic treatment in Swedish sheep herds

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